Transcript: Human XM_011534475.3

PREDICTED: Homo sapiens Fas associated factor family member 2 (FAF2), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
FAF2 (23197)
Length:
1970
CDS:
81..1283

Additional Resources:

NCBI RefSeq record:
XM_011534475.3
NBCI Gene record:
FAF2 (23197)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011534475.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000284973 CGCGCAAGGTTAGCAACAAAC pLKO_005 1489 3UTR 100% 10.800 15.120 N FAF2 n/a
2 TRCN0000191263 CGGTTTACCTATTACACGATA pLKO.1 255 CDS 100% 4.950 6.930 N Faf2 n/a
3 TRCN0000004407 GCTTCCATTCCGGTTTACCTA pLKO.1 245 CDS 100% 3.000 2.400 N FAF2 n/a
4 TRCN0000273433 GCTTCCATTCCGGTTTACCTA pLKO_005 245 CDS 100% 3.000 2.400 N FAF2 n/a
5 TRCN0000004408 CGGCAGAATTTACAGGAGGAA pLKO.1 951 CDS 100% 2.640 2.112 N FAF2 n/a
6 TRCN0000273432 CATTAACCAACTGACATTTAT pLKO_005 719 CDS 100% 15.000 10.500 N FAF2 n/a
7 TRCN0000004406 GCTTTACGAGAGAACACCTAT pLKO.1 615 CDS 100% 4.950 3.465 N FAF2 n/a
8 TRCN0000273377 GCTTTACGAGAGAACACCTAT pLKO_005 615 CDS 100% 4.950 3.465 N FAF2 n/a
9 TRCN0000004405 CCACGACTTCTTATTCTCCTT pLKO.1 1106 CDS 100% 2.640 1.848 N FAF2 n/a
10 TRCN0000273378 CCACGACTTCTTATTCTCCTT pLKO_005 1106 CDS 100% 2.640 1.848 N FAF2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011534475.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02735 pDONR223 100% 89.8% 89.8% None 131_132ins135 n/a
2 ccsbBroad304_02735 pLX_304 0% 89.8% 89.8% V5 131_132ins135 n/a
3 TRCN0000477478 AGACCTCGAGCGATCGCGGAACCC pLX_317 32.7% 89.8% 89.8% V5 131_132ins135 n/a
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