Construct: ORF TRCN0000477480
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003942.1_s317c1
- Derived from:
- ccsbBroadEn_05555
- DNA Barcode:
- TTCCTAAATACAGTTACCTAACCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CATSPER4 (378807)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477480
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 378807 | CATSPER4 | cation channel sperm associ... | NM_198137.1 | 100% | 100% | |
| 2 | human | 378807 | CATSPER4 | cation channel sperm associ... | XM_011541432.3 | 81.7% | 71.6% | 985_986insAGAC;1158_1159ins254 |
| 3 | human | 378807 | CATSPER4 | cation channel sperm associ... | XM_011541433.2 | 67% | 55% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1485
- ORF length:
- 1416
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gagggataat gaaaaggcct ggtggcagca atggacctcc catacaggcc 121 tcgaggggtg gggcgggact caggaggacc gtatggggtt tggaggggca gtagctgcac 181 tgaggggccg cccctctccc ctgcagagta ccattcacga gtcctacggt cggccagagg 241 agcaagtgct catcaaccgc caggaaatca cgaacaaagc ggacgcctgg gacatgcagg 301 agttcatcac tcacatgtac atcaagcagc tgctccgaca ccccgccttc caactgctgc 361 tggccctgct gctggtgatc aatgccatca ccatcgctct ccgtaccaac tcctacctgg 421 accagaaaca ctatgagttg ttctctacca tagatgacat tgtgctgacc atccttcttt 481 gtgaggttct ccttggctgg ctcaatggct tctggatttt ctggaaggac ggctggaaca 541 tcctcaactt cattatcgtc tttatcttgc tcttgcggtt cttcattaat gaaatcaata 601 ttccctccat caactacact ctcagggcgc ttcgtctggt gcatgtgtgc atggcggtgg 661 agcccctcgc ccggatcatc cgcgtcatcc tgcagtcggt gcctgacatg gccaatatca 721 tggtcctcat cctcttcttc atgctggttt tttccgtgtt tggagtaaca ctctttggtg 781 cattcgtgcc caagcatttc cagaacatac aggttgcgct gtacaccctc ttcatctgca 841 tcacccagga cggctgggtg gacatctaca gtgacttcca gacagagaag agggaatatg 901 caatggagat tgggggtgcc atctacttta ccatcttcat caccatcggt gccttcattg 961 gcatcaacct gttcgtcatc gtggtgacca ccaacctGGA GCAAATGATG AAGGCAGGAG 1021 AGCAGGGACA ACAGCAACGA ATAACCTTTA GTGAGACAGG CGCAGAGGAA GAGGAGGAGA 1081 ATGACCAGCT GCCACTGGTG CATTGTGTGG TCGCCCGCTC GGAGAAATCT GGTCTCCTCC 1141 AGGAACCCCT TGCGGGAGGC CCCCTGTCGA ACCTCTCAGA AAACACGTGT GACAACTTTT 1201 GCTTGGTGCT TGAGGCAATA CAGGAGAACC TGAGGCAGTA CAAGGAGATC CGAGATGAAC 1261 TCAACATGAT TGTGGAGGAG GTGCGCGCAA TCCGCTTCAA CCAGGAGCAG GAGTCAGAGG 1321 TGTTGAACAG GCGCTCGTCG ACGAGCGGGT CGTTGGAGAC TACGTCATCC AAGGACATCC 1381 GCCAGATGTC TCAACAGCAA GACTTGCTCA GTGCGCTCGT TAGCATGGAA AAGGTTCATG 1441 ACTCTAGCTC ACAAATACTC CTTAAAAAAC ACAAGAGCAG CCACTTGCCA ACTTTCTTGT 1501 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1561 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1621 GAAAGGACGA TTCCTAAATA CAGTTACCTA ACCAACGCGT TAAGTCgaca atcaacctct 1681 ggattacaaa atttgtgaaa gatt