Construct: ORF TRCN0000477491
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002959.1_s317c1
- Derived from:
- ccsbBroadEn_12722
- DNA Barcode:
- TAGCCATCCGACGGCTTTCAGCGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- COL21A1 (81578)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477491
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 81578 | COL21A1 | collagen type XXI alpha 1 c... | XM_024446561.1 | 99.8% | 99.5% | 908T>G;974G>A |
| 2 | human | 81578 | COL21A1 | collagen type XXI alpha 1 c... | NM_001318753.2 | 73.7% | 73.1% | (many diffs) |
| 3 | human | 81578 | COL21A1 | collagen type XXI alpha 1 c... | NM_001318754.2 | 65.2% | 47.2% | (many diffs) |
| 4 | human | 81578 | COL21A1 | collagen type XXI alpha 1 c... | NM_001318752.2 | 50.2% | 50% | (many diffs) |
| 5 | human | 81578 | COL21A1 | collagen type XXI alpha 1 c... | NM_001318751.2 | 50% | 49.8% | (many diffs) |
| 6 | human | 81578 | COL21A1 | collagen type XXI alpha 1 c... | NM_030820.4 | 50% | 49.8% | (many diffs) |
| 7 | human | 81578 | COL21A1 | collagen type XXI alpha 1 c... | XM_011514924.2 | 50% | 49.8% | (many diffs) |
| 8 | human | 81578 | COL21A1 | collagen type XXI alpha 1 c... | XM_011514925.3 | 50% | 49.8% | (many diffs) |
| 9 | human | 81578 | COL21A1 | collagen type XXI alpha 1 c... | XM_011514926.1 | 50% | 49.8% | (many diffs) |
| 10 | human | 81578 | COL21A1 | collagen type XXI alpha 1 c... | XM_011514927.1 | 50% | 49.8% | (many diffs) |
| 11 | human | 81578 | COL21A1 | collagen type XXI alpha 1 c... | XM_006715223.2 | 48.8% | 48.5% | (many diffs) |
| 12 | human | 81578 | COL21A1 | collagen type XXI alpha 1 c... | NR_134849.2 | 34.5% | (many diffs) | |
| 13 | human | 81578 | COL21A1 | collagen type XXI alpha 1 c... | NR_134850.2 | 34.3% | (many diffs) | |
| 14 | human | 81578 | COL21A1 | collagen type XXI alpha 1 c... | XR_001743657.2 | 34.2% | (many diffs) | |
| 15 | human | 81578 | COL21A1 | collagen type XXI alpha 1 c... | NR_134851.2 | 33.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1509
- ORF length:
- 1440
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gggatatcag ggaattgcag ggacaccagg tgttccagga tctccaggaa 121 tacaaggagc tcgaggacta ccaggttaca aaggagaacc agggcgagat ggtgacaagg 181 gtgatcgtgg acttcctggt tttcctgggc ttcatggcat gccaggatca aagggtgaaa 241 tgggtgccaa aggagacaaa ggatcacctg gattttatgg caaaaagggt gcaaaaggtg 301 aaaaggggaa tgctggcttc cctggcctcc ctggacctgc tggagaacca ggaagacatg 361 gaaaggatgg attaatgggt agtcccggtt tcaagggaga agcaggatcc cctggtgctc 421 cggggcagga tggaacacgg ggagagcctg gaatcccagg atttcctgga aaccgaggat 481 taatgggcca aaagggagaa attgggcctc caggacagca aggaaaaaaa ggagccccag 541 ggatgcctgg tttaatggga agcaatggct caccaggcca gcctggaaca ccgggatcta 601 agggaagcaa aggtgaacct ggaattcaag ggatgcctgg ggcttctggg ctcaagggag 661 aaccaggagc aacgggttcc ccaggagaac caggatacat gggtttaccc gggattcaag 721 gaaaaaaggg ggacaaagga aatcaaggtg aaaaaggtat tcagggtcaa aagggagaaa 781 atggaagaca gggaattcca gggcaacagg gaattcaagg ccatcatggt gcaaaaggag 841 agagaggtga aaagggagaa cctggtgtcc gaggtgccat tggatcaaaa ggagaatctg 901 gggtggatgg cttgatgggg cccgcaggtc ctaaggggca acctggggat ccaggtcctc 961 agggaccccc aggttgggat gggaagcccg gaagagagtt ttcagaacaa tttattcgac 1021 aagtttgcac agatgtaata aaagcccagc taccagtctt acttcagagt ggaagaatta 1081 gaaattgtga tcattgcctg tcccaacatg gctccccggg tattcctggg ccacctggtc 1141 cgataggccc agagggtccc agaggattac ctggtttgcc aggaagagat ggtgttcctg 1201 gattagtggg tgtccctgga cgtccaggtg tcagaggatt aaaaggccta ccaggaagaa 1261 atggggaaaa agggagccaa gggtttgggt atcctggaga acaaggtcct cctggtcccc 1321 caggtccaga gggccctcct ggaataagca aagaaggtcc tccaggagac ccaggtctcc 1381 ctggcaaaga tggagaccat ggaaaacctg gaatccaagg gcaaccaggc cccccaggca 1441 tcTGCGACCC ATCACTATGT TTTAGTGTAA TTGCCAGAAG AGATCCGTTC AGAAAAGGAC 1501 CAAACTATTT GCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1561 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1621 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGATAGCCA TCCGACGGCT TTCAGCGTAC 1681 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt