Construct: ORF TRCN0000477495
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018494.1_s317c1
- Derived from:
- ccsbBroadEn_04068
- DNA Barcode:
- CAATTCCTTCCACCCTTGCTGATA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TTPAL (79183)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477495
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79183 | TTPAL | alpha tocopherol transfer p... | NM_001039199.3 | 100% | 100% | |
| 2 | human | 79183 | TTPAL | alpha tocopherol transfer p... | NM_024331.5 | 100% | 100% | |
| 3 | human | 79183 | TTPAL | alpha tocopherol transfer p... | XM_011529043.3 | 100% | 100% | |
| 4 | human | 79183 | TTPAL | alpha tocopherol transfer p... | XM_011529044.3 | 100% | 100% | |
| 5 | human | 79183 | TTPAL | alpha tocopherol transfer p... | NM_001261839.2 | 90% | 90% | 533_534ins102 |
| 6 | human | 79183 | TTPAL | alpha tocopherol transfer p... | XM_017028063.2 | 90% | 90% | 533_534ins102 |
| 7 | human | 79183 | TTPAL | alpha tocopherol transfer p... | XM_024452000.1 | 90% | 90% | 533_534ins102 |
| 8 | human | 79183 | TTPAL | alpha tocopherol transfer p... | XM_011529045.3 | 89.1% | 89.1% | 639_640ins111 |
| 9 | human | 79183 | TTPAL | alpha tocopherol transfer p... | XM_024452001.1 | 50.8% | 44.5% | (many diffs) |
| 10 | mouse | 76080 | Ttpal | tocopherol (alpha) transfer... | NM_029512.2 | 87.6% | 94.1% | (many diffs) |
| 11 | mouse | 76080 | Ttpal | tocopherol (alpha) transfer... | NM_181734.3 | 87.6% | 94.1% | (many diffs) |
| 12 | mouse | 76080 | Ttpal | tocopherol (alpha) transfer... | XM_006500361.1 | 87.6% | 94.1% | (many diffs) |
| 13 | mouse | 76080 | Ttpal | tocopherol (alpha) transfer... | XM_006500362.1 | 87.6% | 94.1% | (many diffs) |
| 14 | mouse | 76080 | Ttpal | tocopherol (alpha) transfer... | NM_001305803.1 | 86% | 92.4% | (many diffs) |
| 15 | mouse | 76080 | Ttpal | tocopherol (alpha) transfer... | XM_017319317.1 | 86% | 92.4% | (many diffs) |
| 16 | mouse | 76080 | Ttpal | tocopherol (alpha) transfer... | XR_374538.3 | 19.1% | (many diffs) | |
| 17 | mouse | 76080 | Ttpal | tocopherol (alpha) transfer... | NR_131225.1 | 15.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1095
- ORF length:
- 1026
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtccgaagaa agtgactctc tgagaaccag cccttctgtg gcctcactct 121 ctgaaaatga gctgccacca ccacctgagc ctccgggcta tgtgtgctca ctgacagaag 181 acctggtcac caaagcccgg gaagagctgc aggaaaagcc ggaatggaga cttcgagatg 241 tgcaggccct tcgtgacatg gtgcggaagg agtaccccaa cctgagcaca tccctcgacg 301 atgccttcct gctgcgcttc ctccgagccc gcaagtttga ttacgaccgg gccctgcagc 361 tcctcgtcaa ctaccacagc tgtagaagaa gctggcccga agtcttcaat aacttgaagc 421 catcagcctt aaaagatgtc cttgcttccg ggttcctcac cgtgctgccc cacactgacc 481 ccaggggctg ccatgtcgtc tgcatccgcc cagacagatg gataccaagc aactatccaa 541 ttactgaaaa catccgagcc atatacttga ccttagaaaa actcattcag tctgaagaaa 601 cccaggtgaa tggaattgta attcttgcag actacaaagg agtgagttta tcaaaagcat 661 ctcactttgg cccttttata gccaaaaagg tgattggcat ccTCCAGGAT GGTTTCCCCA 721 TTCGGATAAA AGCAGTCCAT GTGGTGAATG AACCTCGAAT ATTTAAAGGC ATTTTTGCCA 781 TCATAAAACC ATTTCTAAAG GAGAAAATAG CAAACAGATT CTTCCTCCAT GGGTCTGACT 841 TGAACTCTCT CCACACAAAC CTTCCAAGAA GCATCCTCCC CAAGGAGTAT GGGGGCACGG 901 CTGGGGAGCT GGACACTGCC ACCTGGAACG CGGTACTGCT GGCTTCAGAA GACGATTTTG 961 TGAAAGAGTT CTGCCAACCT GTTCCTGCCT GTGACAGCAT CCTGGGCCAG ACGCTGCTGC 1021 CCGAGGGCCT GACCTCAGAT GCACAGTGTG ACGACTCCTT GCGAGCTGTG AAGTCACAGC 1081 TGTACTCCTG CTACTTGCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1141 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1201 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CAATTCCTTC CACCCTTGCT 1261 GATAACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt