Construct: ORF TRCN0000477500
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018019.1_s317c1
- Derived from:
- ccsbBroadEn_12014
- DNA Barcode:
- GTGAAATTAGTTGTGCGCCCTTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- WWOX (51741)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477500
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51741 | WWOX | WW domain containing oxidor... | NM_130791.4 | 76.1% | 75.6% | (many diffs) |
| 2 | human | 51741 | WWOX | WW domain containing oxidor... | NR_120436.2 | 61.5% | (many diffs) | |
| 3 | human | 51741 | WWOX | WW domain containing oxidor... | NM_016373.4 | 56.5% | 56.5% | 516_1055del |
| 4 | human | 51741 | WWOX | WW domain containing oxidor... | NM_001291997.2 | 29.2% | 29.2% | 0_1ins339;177_716del |
| 5 | mouse | 80707 | Wwox | WW domain-containing oxidor... | XM_006531515.3 | 74.7% | 70.7% | (many diffs) |
| 6 | mouse | 80707 | Wwox | WW domain-containing oxidor... | NM_019573.3 | 49.1% | 53.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 768
- ORF length:
- 702
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc agcgctgcgc tacgcggggc tggacgacac ggacagtgag gacgagctgc 121 ctccgggctg ggaggagaga accaccaagg acggctgggt ttactacgcc aatcacaccg 181 aggagaagac tcagtgggaa catccaaaaa ctggaaaaag aaaacgagtg gcaggagatt 241 tgccatacgg atgggaacaa gaaactgatg agaacggaca agtgtttttt gttgaccata 301 taaataaaag aaccacctac ttggacccaa gactggcgtt tactgtggat gataatccga 361 cCAAGCCAAC CACCCGGCAA AGATACGACG GCAGCACCAC TGCCATGGAA ATTCTCCAGG 421 GCCGGGATTT CACTGGCAAA GTGGTTGTGG TCACTGGAGC TAATTCAGGA ATAGGGTTCG 481 AAACCGCCAA GTCTTTTGCC CTCCATGGTG CACATGTGAT CTTGGCCTGC AGGAACATGG 541 CAAGGGCGAG TGAAGCAGTG TCACGCATTT TAGAAGAATG GCAACAGGGA GCTGCCACCA 601 CCGTGTACTG TGCTGCTGTC CCAGAACTGG AGGGTCTGGG AGGGATGTAC TTCAACAACT 661 GCTGCCGCTG CATGCCCTCA CCAGAAGCTC AGAGCGAAGA GACGGCCCGG ACCCTGTGGG 721 CGCTCAGCGA GAGGCTGATC CAAGAACGGC TTGGCAGCCA GTCCGGCTAC CCAACTTTCT 781 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 841 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 901 GTGGAAAGGA CGAGTGAAAT TAGTTGTGCG CCCTTTTACG CGTTAAGTCg acaatcaacc 961 tctggattac aaaatttgtg aaagatt