Construct: ORF TRCN0000477509
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011232.1_s317c1
- Derived from:
- ccsbBroadEn_09367
- DNA Barcode:
- TATCCGCCCATCTGACCATCCAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SYTL4 (94121)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477509
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 94121 | SYTL4 | synaptotagmin like 4 | NM_001129896.3 | 99.9% | 99.8% | 1258A>G |
| 2 | human | 94121 | SYTL4 | synaptotagmin like 4 | NM_001174068.2 | 99.9% | 99.8% | 1258A>G |
| 3 | human | 94121 | SYTL4 | synaptotagmin like 4 | NM_001370160.1 | 99.9% | 99.8% | 1258A>G |
| 4 | human | 94121 | SYTL4 | synaptotagmin like 4 | NM_001370161.1 | 99.9% | 99.8% | 1258A>G |
| 5 | human | 94121 | SYTL4 | synaptotagmin like 4 | NM_001370163.1 | 99.9% | 99.8% | 1258A>G |
| 6 | human | 94121 | SYTL4 | synaptotagmin like 4 | NM_001370164.1 | 99.9% | 99.8% | 1258A>G |
| 7 | human | 94121 | SYTL4 | synaptotagmin like 4 | NM_001370165.1 | 99.9% | 99.8% | 1258A>G |
| 8 | human | 94121 | SYTL4 | synaptotagmin like 4 | NM_001370166.1 | 99.9% | 99.8% | 1258A>G |
| 9 | human | 94121 | SYTL4 | synaptotagmin like 4 | NM_001370167.1 | 99.9% | 99.8% | 1258A>G |
| 10 | human | 94121 | SYTL4 | synaptotagmin like 4 | NM_001370168.1 | 99.9% | 99.8% | 1258A>G |
| 11 | human | 94121 | SYTL4 | synaptotagmin like 4 | NM_001370169.1 | 99.9% | 99.8% | 1258A>G |
| 12 | human | 94121 | SYTL4 | synaptotagmin like 4 | NM_080737.3 | 99.9% | 99.8% | 1258A>G |
| 13 | human | 94121 | SYTL4 | synaptotagmin like 4 | NM_001370162.1 | 94.7% | 90.5% | (many diffs) |
| 14 | human | 94121 | SYTL4 | synaptotagmin like 4 | XM_017029966.1 | 94.7% | 90.5% | (many diffs) |
| 15 | human | 94121 | SYTL4 | synaptotagmin like 4 | XM_017029967.2 | 94.7% | 90.5% | (many diffs) |
| 16 | human | 94121 | SYTL4 | synaptotagmin like 4 | XM_017029968.1 | 94.7% | 90.5% | (many diffs) |
| 17 | human | 94121 | SYTL4 | synaptotagmin like 4 | XM_017029969.1 | 94.7% | 90.5% | (many diffs) |
| 18 | human | 94121 | SYTL4 | synaptotagmin like 4 | XM_017029970.1 | 94.7% | 90.5% | (many diffs) |
| 19 | mouse | 27359 | Sytl4 | synaptotagmin-like 4 | NM_013757.2 | 89.3% | 90.1% | (many diffs) |
| 20 | mouse | 27359 | Sytl4 | synaptotagmin-like 4 | XM_006528551.2 | 89.3% | 90.1% | (many diffs) |
| 21 | mouse | 27359 | Sytl4 | synaptotagmin-like 4 | NM_001290717.1 | 64.4% | 63.7% | (many diffs) |
| 22 | mouse | 27359 | Sytl4 | synaptotagmin-like 4 | NM_001290718.1 | 64.4% | 63.7% | (many diffs) |
| 23 | mouse | 27359 | Sytl4 | synaptotagmin-like 4 | NM_001290719.1 | 63.9% | 63.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2079
- ORF length:
- 2013
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc ggagttactg gacctttctt ttctgtctga ggaggaaaag gatttgattc 121 tcagtgttct acagcgagat gaagaggtcc ggaaagcaga tgagaaaagg attaggcgac 181 taaagaatga gttactggag ataaaaagga aaggggccaa gaggggcagc caacactaca 241 gtgatcggac ctgtgcccgg tgccaggaga gcctgggccg tttgagtccc aaaaccaata 301 cttgtcgggg ttgtaatcac ctggtgtgtc gggactgccg catacaggaa agcaatggta 361 cctggaggtg caaggtgtgc gccaaggaaa tagagttgaa gaaagcaact ggggactggt 421 tttatgacca gaaagtgaat cgctttgctt accgcacagg tagtgagata atcaggatgt 481 ccctgcgcca caaacctgca gtgagtaaaa gagagacagt gggacagtcc ctccttcatc 541 agacacagat gggtgacatc tggccaggaa gaaagatcat tcaggagcgg cagaaggagc 601 ccagtgtgct atttgaagtg ccaaagctga aaagtggaaa gagtgcattg gaagctgaga 661 gtgagagtct ggatagcttc acagctgact cggatagcac ctccaggaga gactctctgg 721 ataaatctgg cctctttcca gaatggaaga agatgtctgc tcccaaatct caagtagaaa 781 aggaaactca gcctggaggt caaaatgtgg tatttgtgga tgagggtgag atgatattta 841 agaagaacac cagaaaaatc ctcaggcctt cagagtacac taaatctgtg atagatcttc 901 gcccagaaga tgtggtacat gaaagtggct ccttgggaga cagaagcaaa tccgtcccag 961 gcctcaatgt ggatatggaa gaggaagaag aagaagaaga cattgaccac ctagtgaagt 1021 tacatcgcca gaagctagcc agaagcagca tgcaaagtgg ctcctccatg agtacgatcg 1081 gcagcatgat gagcatctac agtgaagctg gtgatttcgg gaacatcttt gtgactggca 1141 ggattgcctt ttccctgaag tatgagcagc aaacccagag tctggttgtc catgtgaagg 1201 agtgccatca gctggcctat gctgatgaag ccaagaagcg ctctaaccca tatgtgaaga 1261 cttaccttct gcctgacaag tcccgccaag gaaaaagaaa aaccagcatc aagcgggaca 1321 ctgttaatcc actatatgat gagacgctga ggtatgagat cccagaatct ctcctggccc 1381 agaggaccct gcagttctca gtttggcatc atggtcgttt tggcagaaac actttccttg 1441 gagaggcaga gatccagatg gattcctgga agcttgataa gaaactggat cattgcctcc 1501 ctttacatgg aaagatcagt gctgagtccc cgactggctt gccatcacac aaaggcgagt 1561 tggtggtttc attgaaatac atcccagcct ccaaaacccc tgttggaggt gaccggaaaa 1621 agagtaaagg tggggaaggg ggagagctcc aggtgtggat caaagaagcc aagaaCTTGA 1681 CGGCTGCCAA AGCAGGAGGG ACTTCAGACA GCTTTGTCAA GGGATACCTC CTTCCCATGA 1741 GGAACAAGGC CAGTAAACGT AAAACTCCTG TGATGAAGAA GACCCTGAAT CCTCACTACA 1801 ACCATACATT TGTCTACAAT GGTGTGAGGC TGGAAGATCT ACAGCATATG TGCCTGGAAC 1861 TGACTGTGTG GGACCGGGAG CCCCTGGCCA GCAATGACTT CCTGGGAGGG GTCAGGCTGG 1921 GTGTTGGCAC TGGGATCAGT AATGGGGAAG TGGTGGACTG GATGGACTCG ACTGGGGAAG 1981 AAGTGAGCCT GTGGCAGAAG ATGCGACAGT ACCCAGGGTC TTGGGCAGAA GGGACTCTGC 2041 AGCTCCGTTC CTCAATGGCC AAGCAGAAGC TGGGTTTATG CCCAACTTTC TTGTACAAAG 2101 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 2161 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 2221 ACGATATCCG CCCATCTGAC CATCCAACAC GCGTTAAGTC gacaatcaac ctctggatta 2281 caaaatttgt gaaagatt