Construct: ORF TRCN0000477514
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010557.1_s317c1
- Derived from:
- ccsbBroadEn_04873
- DNA Barcode:
- CGACCTGAAACTGACTGTGTTTGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CD200R1 (131450)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477514
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 131450 | CD200R1 | CD200 receptor 1 | NM_138806.4 | 100% | 100% | |
| 2 | human | 131450 | CD200R1 | CD200 receptor 1 | NM_170780.3 | 93.3% | 93.3% | 66_67ins69 |
| 3 | human | 344807 | CD200R1L | CD200 receptor 1 like | NM_001008784.2 | 64.6% | 54.5% | (many diffs) |
| 4 | human | 344807 | CD200R1L | CD200 receptor 1 like | NM_001199215.1 | 62% | 51.6% | (many diffs) |
| 5 | human | 344807 | CD200R1L | CD200 receptor 1 like | NM_001370552.1 | 62% | 51.6% | (many diffs) |
| 6 | human | 344807 | CD200R1L | CD200 receptor 1 like | XM_011512763.3 | 62% | 51.6% | (many diffs) |
| 7 | human | 131450 | CD200R1 | CD200 receptor 1 | NM_138939.3 | 53% | 48.6% | (many diffs) |
| 8 | human | 131450 | CD200R1 | CD200 receptor 1 | NM_138940.3 | 46.4% | 42.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1110
- ORF length:
- 1044
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct ctgcccttgg agaactgcta acctagggct actgttgatt ttgactatct 121 tcttagtggc cgaagcggag ggtgctgctc aaccaaacaa ctcattaatg ctgcaaacta 181 gcaaggagaa tcatgcttta gcttcaagca gtttatgtat ggatgaaaaa cagattacac 241 agaactactc gaaagtactc gcagaagtta acacttcatg gcctgtaaag atggctacaa 301 atgctgtgct ttgttgccct cctatcgcat taagaaattt gatcataata acatgggaaa 361 taatcctgag aggccagcct tcctgcacaa aagcctacaa gaaagaaaca aatgagacca 421 aggaaaccaa ctgtactgat gagagaataa cctgggtctc cagacctgat cagaattcgg 481 accttcagat tcgtaccgtg gccatcactc atgacgggta ttacagatgc ataatggtaa 541 cacctgatgg gaatttccat cgtggatatc acctccaagt gttagttaca cctgaagtga 601 ccctgtttca aaacaggaat agaactgcag tatgcaaggc agttgcaggg aagccagctg 661 cgcataTCTC CTGGATCCCA GAGGGCGATT GTGCCACTAA GCAAGAATAC TGGAGCAATG 721 GCACAGTGAC TGTTAAGAGT ACATGCCACT GGGAGGTCCA CAATGTGTCT ACCGTGACCT 781 GCCACGTCTC CCATTTGACT GGCAACAAGA GTCTGTACAT AGAGCTACTT CCTGTTCCAG 841 GTGCCAAAAA ATCAGCAAAA TTATATATTC CATATATCAT CCTTACTATT ATTATTTTGA 901 CCATCGTGGG ATTCATTTGG TTGTTGAAAG TCAATGGCTG CAGAAAATAT AAATTGAATA 961 AAACAGAATC TACTCCAGTT GTTGAGGAGG ATGAAATGCA GCCCTATGCC AGCTACACAG 1021 AGAAGAACAA TCCTCTCTAT GATACTACAA ACAAGGTGAA GGCATCTGAG GCATTACAAA 1081 GTGAAGTTGA CACAGACCTC CATACTTTAT ACCCAACTTT CTTGTACAAA GTGGTTGATA 1141 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1201 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGACGACC 1261 TGAAACTGAC TGTGTTTGCA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1321 tgaaagatt