Construct: ORF TRCN0000477517

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018109.1_s317c1
Derived from:
ccsbBroadEn_03378
DNA Barcode:
AATGCAGTCTCAATCATATTCTAT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ERAP1 (51752)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000477517
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 51752 ERAP1 endoplasmic reticulum amino... NM_001040458.3 100% 100%
2 human 51752 ERAP1 endoplasmic reticulum amino... NM_001198541.2 100% 100%
3 human 51752 ERAP1 endoplasmic reticulum amino... XM_011543486.3 100% 100%
4 human 51752 ERAP1 endoplasmic reticulum amino... NM_001349244.1 99.1% 99% 2818_2819insGT;2822_2844del
5 human 51752 ERAP1 endoplasmic reticulum amino... NM_016442.4 99.1% 99% 2818_2819insGT;2822_2844del
6 human 51752 ERAP1 endoplasmic reticulum amino... XM_005272015.5 98.9% 98.8% 2820_2824delTTCTC;2828_2853delinsG
7 human 51752 ERAP1 endoplasmic reticulum amino... XM_005272016.4 98.9% 98.8% 2820_2824delTTCTC;2828_2853delinsG
8 human 51752 ERAP1 endoplasmic reticulum amino... XM_011543480.2 98.9% 98.8% 2820_2824delTTCTC;2828_2853delinsG
9 human 51752 ERAP1 endoplasmic reticulum amino... XM_011543481.2 98.9% 98.8% 2820_2824delTTCTC;2828_2853delinsG
10 human 51752 ERAP1 endoplasmic reticulum amino... XM_011543484.2 98.9% 98.8% 2820_2824delTTCTC;2828_2853delinsG
11 human 51752 ERAP1 endoplasmic reticulum amino... XM_011543485.2 98.9% 98.8% 2820_2824delTTCTC;2828_2853delinsG
12 human 51752 ERAP1 endoplasmic reticulum amino... XM_017009581.1 98.9% 98.8% 2820_2824delTTCTC;2828_2853delinsG
13 human 51752 ERAP1 endoplasmic reticulum amino... XM_024446113.1 98.9% 98.8% 2820_2824delTTCTC;2828_2853delinsG
14 human 51752 ERAP1 endoplasmic reticulum amino... XM_017009583.2 60.5% 60.4% 0_1ins1095;1725_1729delTTCTC;1733_1758delinsG
15 human 51752 ERAP1 endoplasmic reticulum amino... XR_001742119.2 43.9% (many diffs)
16 mouse 80898 Erap1 endoplasmic reticulum amino... NM_030711.4 84.9% 84.2% (many diffs)
17 mouse 80898 Erap1 endoplasmic reticulum amino... XM_006517476.3 84.9% 84.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2889
ORF length:
2823
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggt gtttctgccc ctcaaatggt cccttgcaac catgtcattt ctactttcct
121cactgttggc tctcttaact gtgtccactc cttcatggtg tcagagcact gaagcatctc
181caaaacgtag tgatgggaca ccatttcctt ggaataaaat acgacttcct gagtacgtca
241tcccagttca ttatgatctc ttgatccatg caaaccttac cacgctgacc ttctggggaa
301ccacgaaagt agaaatcaca gccagtcagc ccaccagcac catcatcctg catagtcacc
361acctgcagat atctagggcc accctcagga agggagctgg agagaggcta tcggaagaac
421ccctgcaggt cctggaacac ccccgtcagg agcaaattgc actgctggct cccgagcccc
481tccttgtcgg gctcccgtac acagttgtca ttcactatgc tggcaatctt tcggagactt
541tccacggatt ttacaaaagc acctacagaa ccaaggaagg ggaactgagg atactagcat
601caacacaatt tgaacccact gcagctagaa tggcctttcc ctgctttgat gaacctgcct
661tcaaagcaag tttctcaatc aaaattagaa gagagccaag gcacctagcc atctccaata
721tgccattggt gaaatctgtg actgttgctg aaggactcat agaagaccat tttgatgtca
781ctgtgaagat gagcacctat ctggtggcct tcatcatttc agattttgag tctgtcagca
841agataaccaa gagtggagtc aaggtttctg tttatgctgt gccagacaag ataaatcaag
901cagattatgc actggatgct gcggtgactc ttctagaatt ttatgaggat tatttcagca
961taccgtatcc cctacccaaa caagatcttg ctgctattcc cgactttcag tctggtgcta
1021tggaaaactg gggactgaca acatatagag aatctgctct gttgtttgat gcagaaaagt
1081cttctgcatc aagtaagctt ggcatcacaa tgactgtggc ccatgaactg gctcaccagt
1141ggtttgggaa cctggtcact atggaatggt ggaatgatct ttggctaaat gaaggatttg
1201ccaaatttat ggagtttgtg tctgtcagtg tgacccatcc tgaactgaaa gttggagatt
1261atttctttgg caaatgtttt gacgcaatgg aggtagatgc tttaaattcc tcacaccctg
1321tgtctacacc tgtggaaaat cctgctcaga tccgggagat gtttgatgat gtttcttatg
1381ataagggagc ttgtattctg aatatgctaa gggagtatct tagtgctgac gcatttaaaa
1441gtggtattgt acagtatctc cagaagcata gctataaaaa tacaaaaaac gaggacctgt
1501gggatagtat ggcaagtatt tgccctacag atggtgtaaa agggatggat ggcttttgct
1561ctagaagtca acattcatct tcatcctcac attggcatca ggaaggggtg gatgtgaaaa
1621ccatgatgaa cacttggaca ctgcagaagg gttttcccct aataaccatc acagtgaggg
1681ggaggaatgt acacatgaag caagagcact acatgaaggg ctctgacggc gccccggaca
1741ctgggtacct gtggcatgtt ccattgacat tcatcaccag caaatccgac atggtccatc
1801gatttttgct aaaaacaaaa acagatgtgc tcatcctccc agaagaggtg gaatggatca
1861aatttaatgt gggcatgaat ggctattaca ttgtgcatta cgaggatgat ggatgggact
1921ctttgactgg ccttttaaaa ggaacacaca cagcagtcag cagtaatgat cgggcgagtc
1981tcattaacaa tgcatttcag ctcgtcagca ttgggaagct gtccattgaa aaggccttgg
2041atttatccct gtacttgaaa catgaaactg aaattatgcc cgtgtttcaa ggtttgaatg
2101agctgattcc tatgtataag ttaatggaga aaagagatat gaatgaagtg gaaactcaat
2161tcaaggcctt cctcatcagg ctgctaaggg acctcattga taagcagaca tggacagacg
2221agggctcagt ctcagagcga atgctgcgga gtcaactact actcctcgcc tgtgtgcaca
2281actatcagcc gtgcgtacag agggcagaag gctatttcag aaagtggaag gaatccaatg
2341gaaacttgag cctgcctgtc gacgtgacct tggcagtgtt tgctgtgggg gcccagagca
2401cagaaggctg ggattttctt tatagtaaat aTCAGTTTTC TTTGTCCAGT ACTGAGAAAA
2461GCCAAATTGA ATTTGCCCTC TGCAGAACCC AAAATAAGGA AAAGCTTCAA TGGCTACTAG
2521ATGAAAGCTT TAAGGGAGAT AAAATAAAAA CTCAGGAGTT TCCACAAATT CTTACACTCA
2581TTGGCAGGAA CCCAGTAGGA TACCCACTGG CCTGGCAATT TCTGAGGAAA AACTGGAACA
2641AACTTGTACA AAAGTTTGAA CTTGGCTCAT CTTCCATAGC CCACATGGTA ATGGGTACAA
2701CAAATCAATT CTCCACAAGA ACACGGCTTG AAGAGGTAAA AGGATTCTTC AGCTCTTTGA
2761AAGAAAATGG TTCTCAGCTC CGTTGTGTCC AACAGACAAT TGAAACCATT GAAGAAAACA
2821TCGGTTGGAT GGATAAGAAT TTTGATAAAA TCAGAGTGTG GCTGCAAAGT GAAAAGCTTG
2881AACGTATGTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC
2941CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA
3001TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAAATGCA GTCTCAATCA TATTCTATAC
3061GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt

Download FASTA (ORF) (Full)