Construct: ORF TRCN0000477526
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011424.1_s317c1
- Derived from:
- ccsbBroadEn_05370
- DNA Barcode:
- GACTTGTAGAAGAGTCAATGTATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MILR1 (284021)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477526
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 284021 | MILR1 | mast cell immunoglobulin li... | NM_001085423.2 | 100% | 100% | |
| 2 | human | 284021 | MILR1 | mast cell immunoglobulin li... | NM_001369493.1 | 100% | 100% | |
| 3 | human | 284021 | MILR1 | mast cell immunoglobulin li... | XM_024450706.1 | 89% | 89% | 1_126del |
| 4 | human | 284021 | MILR1 | mast cell immunoglobulin li... | XM_024450707.1 | 89% | 89% | 1_126del |
| 5 | human | 284021 | MILR1 | mast cell immunoglobulin li... | XM_024450708.1 | 89% | 89% | 1_126del |
| 6 | human | 284021 | MILR1 | mast cell immunoglobulin li... | XM_024450709.1 | 89% | 89% | 1_126del |
| 7 | human | 284021 | MILR1 | mast cell immunoglobulin li... | XM_017024487.1 | 82.9% | 82.7% | 0_1ins174;2T>A |
| 8 | human | 284021 | MILR1 | mast cell immunoglobulin li... | NM_001291317.2 | 73.7% | 73.7% | 96_97ins270 |
| 9 | human | 284021 | MILR1 | mast cell immunoglobulin li... | XR_002957990.1 | 72.5% | 1_147del;1177_1419del | |
| 10 | human | 284021 | MILR1 | mast cell immunoglobulin li... | NM_001291316.2 | 72.3% | 72.3% | 366_367ins285 |
| 11 | human | 284021 | MILR1 | mast cell immunoglobulin li... | XR_002957989.1 | 69.7% | 1_147del;1177_1475del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1098
- ORF length:
- 1029
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtggagccat ttgaacaggc tcctcttctg gagcatattt tcttctgtca 121 cttgtagaaa agctgtattg gattgtgagg caatgaaaac aaatgaattc ccttctccat 181 gtttggactc aaagactaag gtggttatga agggtcaaaa tgtatctatg ttttgttccc 241 ataagaacaa atcactgcag atcacctatt cattgtttcg acgtaagaca cacctgggaa 301 cccaggatgg aaaaggtgaa cctgcgattt ttaacctaag catcacagaa gcccatgaat 361 caggccccta caaatgcaaa gcccaagtta ccagctgttc aaaatacagt cgtgacttca 421 gcttcacgat tgtcgacccg gtgacttccc cagtgctgaa cattatggtc attcaaacag 481 aaacagaccg acatataaca ttacattgcc tctcagtcaa tggctcgctg cccatcaatt 541 acactttctt tgaaaaccat gttgccatat caccagctat ttccaagtat gacagggagc 601 ctgctgaatt taacttaacc aagaagaaTC CTGGAGAAGA GGAAGAGTAT AGGTGTGAAG 661 CTAAAAACAG ATTGCCTAAC TATGCAACAT ACAGTCACCC TGTCACCATG CCCTCAACAG 721 GCGGAGACAG CTGTCCTTTC TGTCTGAAGC TACTACTTCC AGGGTTATTA CTGTTGCTGG 781 TGGTGATAAT CCTAATTCTG GCTTTTTGGG TACTGCCCAA ATACAAAACA AGAAAAGCTA 841 TGAGAAATAA TGTGCCCAGG GACCGTGGAG ACACAGCCAT GGAAGTTGGA ATCTATGCAA 901 ATATCCTTGA AAAACAAGCA AAGGAGGAAT CTGTGCCAGA AGTGGGATCC AGGCCGTGTG 961 TTTCCACAGC CCAAGATGAG GCCAAACACT CCCAGGAGCT ACAGTATGCC ACCCCCGTGT 1021 TCCAGGAGGT GGCACCAAGA GAGCAAGAAG CCTGTGATTC TTATAAATCT GGATATGTCT 1081 ATTCTGAACT CAACTTCTTG CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1141 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1201 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAGACTTGT AGAAGAGTCA 1261 ATGTATTACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt