Construct: ORF TRCN0000477531
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014218.1_s317c1
- Derived from:
- ccsbBroadEn_04416
- DNA Barcode:
- GCGGAGATACGACCCTACCACACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- COX4I2 (84701)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477531
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84701 | COX4I2 | cytochrome c oxidase subuni... | NM_032609.3 | 99.8% | 99.4% | 309G>T |
| 2 | human | 84701 | COX4I2 | cytochrome c oxidase subuni... | XM_005260579.4 | 84.8% | 77.7% | (many diffs) |
| 3 | human | 84701 | COX4I2 | cytochrome c oxidase subuni... | XM_005260581.3 | 74.2% | 74.2% | 247_248ins132 |
| 4 | human | 84701 | COX4I2 | cytochrome c oxidase subuni... | XM_005260580.4 | 61.1% | 55% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 582
- ORF length:
- 513
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gctccccaga gctgcctgga gcttggtgct gaggaaaggt ggaggtggaa 121 gacgagggat gcacagctca gaaggcacca cccgtggtgg ggggaagatg tccccctaca 181 ccaactgcta tgcccagcgc tactacccca tgccagaaga gcccttctgc acagaactca 241 acgctgagga gcaggcccTG AAGGAGAAGG AGAAGGGAAG CTGGACCCAG CTGACCCACG 301 CCGAAAAGGT GGCCTTGTAC CGGCTCCAGT TCAATGAGAC CTTTGCGGAG ATGAACCGTC 361 GCTCCAATGA GTGGAATACA GTGATGGGTT GTGTCTTCTT CTTCATTGGA TTCGCAGCTC 421 TGGTGATTTG GTGGCAGCGG GTCTACGTAT TTCCTCCAAA GCCGATCACC TTGACGGACG 481 AGCGGAAAGC CCAGCAGCTG CAGCGCATGC TGGACATGAA GGTGAATCCT GTGCAGGGCC 541 TGGCCTCCCG CTGGGACTAT GAGAAGAAGC AGTGGAAGAA GTTGCCAACT TTCTTGTACA 601 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 661 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 721 AGGACGAGCG GAGATACGAC CCTACCACAC CACGCGTTAA GTCgacaatc aacctctgga 781 ttacaaaatt tgtgaaagat t