Construct: ORF TRCN0000477547
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005086.1_s317c1
- Derived from:
- ccsbBroadEn_13265
- DNA Barcode:
- GAGAAACTAACTCCCATTCCAATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ANKAR (150709)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477547
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 150709 | ANKAR | ankyrin and armadillo repea... | XM_017003414.2 | 73.4% | 73.4% | (many diffs) |
| 2 | human | 150709 | ANKAR | ankyrin and armadillo repea... | XM_011510689.3 | 70.7% | 70.7% | (many diffs) |
| 3 | human | 150709 | ANKAR | ankyrin and armadillo repea... | XM_011510688.1 | 68.6% | 68.6% | (many diffs) |
| 4 | human | 150709 | ANKAR | ankyrin and armadillo repea... | XM_011510687.2 | 65.5% | 65.5% | (many diffs) |
| 5 | human | 150709 | ANKAR | ankyrin and armadillo repea... | XM_017003417.1 | 64.1% | 62.3% | (many diffs) |
| 6 | human | 150709 | ANKAR | ankyrin and armadillo repea... | XM_011510686.2 | 62.1% | 62% | (many diffs) |
| 7 | human | 150709 | ANKAR | ankyrin and armadillo repea... | XM_011510685.2 | 61.3% | 61.2% | (many diffs) |
| 8 | human | 150709 | ANKAR | ankyrin and armadillo repea... | XM_011510680.1 | 61.2% | 61.2% | (many diffs) |
| 9 | human | 150709 | ANKAR | ankyrin and armadillo repea... | XM_011510681.1 | 61.2% | 61.2% | (many diffs) |
| 10 | human | 150709 | ANKAR | ankyrin and armadillo repea... | XM_011510682.2 | 61.2% | 61.2% | (many diffs) |
| 11 | human | 150709 | ANKAR | ankyrin and armadillo repea... | XM_017003413.1 | 61.2% | 61.2% | (many diffs) |
| 12 | human | 150709 | ANKAR | ankyrin and armadillo repea... | XR_001738642.1 | 59.7% | 1_1282del;3686_4025del | |
| 13 | human | 150709 | ANKAR | ankyrin and armadillo repea... | XM_011510677.2 | 57.8% | 57.7% | (many diffs) |
| 14 | human | 150709 | ANKAR | ankyrin and armadillo repea... | XR_922865.2 | 57.5% | (many diffs) | |
| 15 | human | 150709 | ANKAR | ankyrin and armadillo repea... | XR_001738640.1 | 57.3% | (many diffs) | |
| 16 | human | 150709 | ANKAR | ankyrin and armadillo repea... | XM_011510676.2 | 57% | 56.9% | (many diffs) |
| 17 | human | 150709 | ANKAR | ankyrin and armadillo repea... | XR_922866.1 | 56.7% | 1_1282del;3636_3797del;3848_4234del | |
| 18 | human | 150709 | ANKAR | ankyrin and armadillo repea... | XM_024452719.1 | 56.5% | 56.5% | 1_708del;2468_2469delTGinsA;2472_2472delTins641 |
| 19 | human | 150709 | ANKAR | ankyrin and armadillo repea... | XR_001738641.1 | 55.9% | (many diffs) | |
| 20 | human | 150709 | ANKAR | ankyrin and armadillo repea... | XM_011510673.2 | 54.8% | 54.8% | (many diffs) |
| 21 | human | 150709 | ANKAR | ankyrin and armadillo repea... | XR_001738643.1 | 54.6% | 1_1282del;3207_3208ins33;3653_4303del | |
| 22 | human | 150709 | ANKAR | ankyrin and armadillo repea... | XM_011510679.2 | 54.5% | 50.7% | (many diffs) |
| 23 | human | 150709 | ANKAR | ankyrin and armadillo repea... | NM_144708.3 | 54.1% | 54% | (many diffs) |
| 24 | human | 150709 | ANKAR | ankyrin and armadillo repea... | XM_011510674.1 | 54.1% | 54% | (many diffs) |
| 25 | human | 150709 | ANKAR | ankyrin and armadillo repea... | XM_011510675.2 | 52.4% | 52.3% | (many diffs) |
| 26 | human | 150709 | ANKAR | ankyrin and armadillo repea... | XR_001738644.1 | 52.2% | (many diffs) | |
| 27 | human | 150709 | ANKAR | ankyrin and armadillo repea... | XM_011510691.2 | 50.8% | 49.2% | (many diffs) |
| 28 | human | 150709 | ANKAR | ankyrin and armadillo repea... | XM_011510692.2 | 49.6% | 49.5% | (many diffs) |
| 29 | human | 150709 | ANKAR | ankyrin and armadillo repea... | XM_017003415.1 | 47.1% | 47% | (many diffs) |
| 30 | human | 150709 | ANKAR | ankyrin and armadillo repea... | XM_011510693.2 | 44.4% | 44.3% | (many diffs) |
| 31 | human | 150709 | ANKAR | ankyrin and armadillo repea... | XM_011510694.2 | 43.7% | 43.5% | (many diffs) |
| 32 | human | 150709 | ANKAR | ankyrin and armadillo repea... | XM_017003416.1 | 43.7% | 43.5% | (many diffs) |
| 33 | human | 150709 | ANKAR | ankyrin and armadillo repea... | XM_017003418.1 | 40.8% | 40.6% | (many diffs) |
| 34 | human | 150709 | ANKAR | ankyrin and armadillo repea... | XM_017003419.1 | 39.9% | 39.7% | (many diffs) |
| 35 | human | 150709 | ANKAR | ankyrin and armadillo repea... | XM_024452718.1 | 39.4% | 39.3% | (many diffs) |
| 36 | human | 150709 | ANKAR | ankyrin and armadillo repea... | XM_017003420.1 | 26.7% | 26.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2472
- ORF length:
- 2403
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaaatatgct gaaaatatta tgctaaagtt aacattcagt accacacaaa 121 ttcaacagta tgaaaatgtc tttatatttg aaacaggcta ttggcttact aatgctataa 181 aatataatca ggattatctt gatatctgta cctaccagag actacagcaa agattatatc 241 ttcaaaaaaa gattattcaa aaacactttg agaagaaaaa agatatcaga agagggatag 301 gatacctaaa gttaatatgt tttctgattc catttctact gagtttaaag aagaaaatga 361 aagttccata tttaagtagt ctgcttcagc ctttttcaga tgacaaggtc aagacagagc 421 gagaattgcc tccatttatt tatggaagag attttaaatg ccagaatttt cactacaaag 481 agaatcaata ttttcatgtt catggaggaa ttgaatttga tatcagcacc ccttcaattg 541 agaatgcctt ggaagatttt cagaaaaatt tagaaaaaat acgagattgt gctgctaata 601 catttataga agattcagga tataaagaat attactcaat accagtcatg gaatttcatg 661 gaaaaagcta ctatgtgatc tattttgaac tagaaacttt ctatcagcaa ctatataaga 721 cacagtggtg gggagccata aatgaaatag tgaacaatct gagactgaaa agacttccac 781 tgacagatgc tcaattacat gaacaattta agaaaaagct tggtttcaaa agagctatga 841 aatgcaagag tattccattt ggtatgaagt ccgctgttga aagagggttg tctgcagttt 901 tccacacatt tagccgtaaa acctcaagct caacaatcaa tgtttcagat gaagcaggtt 961 atactatttt tcatcatgct gccctgcaca acagagtttc tattatatgt caactgtgca 1021 atgctaactt caaggtcaac cagaggcgct ttgttacgtt cagccaaggt ccaacacctc 1081 tacaccttgc tgcacaggct tgctcattag aaacaacagt ttgtctactg tgttccaaag 1141 ctgattacac gctttctgaa aaaagaggct ggatgccgat tcactttgcc gctttctatg 1201 acaacgtttg catcattatt gctctctgta ggaaggatcc tagtttgcta gaagctgagg 1261 caacagctga gaatcagtgc actccactgt tacttgctgc cacttcagga gcactggaca 1321 ctattcaata cctgttttct atcggtgcta actggagaaa aacagatatt aaaggaaata 1381 atataatcca tttatcagtg ttaacctttc atacagaggt tctcaaatat ataataaaat 1441 taaatattcc tgaactccca gtgtggaaaa ctttggtaga aatgttacag tgtgaaagct 1501 ataaacgaag gatgatggcc gtcatgtcct tggaagtaat ttgcttagca aatgatcaat 1561 actggagatg tattttggat gcaggcacca ttcctgcctt aatcaatcta ttaaaaagtt 1621 ccaaaataaa actgcagtgc aaaactgttg ggttattgag taatatctca acccacaaaa 1681 gtgcagtgca tgctttggta gaagcgggag gcattccatc tctaatcaac ctactggttt 1741 gtgatgagcc tgaagtacac tctcgctgtg ctgtcattct atatgatatt gctcaatgtg 1801 aaaacaagga tgttattgcc aaatataatg gaatcccaag cctgataaat ctattgaact 1861 taaacataga aaatgtgcta gtaaatgtaa tgaactgtat acgggtattg tgtataggaa 1921 atgaaaacaa tcaaagagct gtgagagaac ataaaggcct cccatatctt atcagatttc 1981 TGAGTTCTGA TTCAGGTTTA TTTAACTTGT TCTTAAATTC ATTTGAAGAT GTGTTGAAGG 2041 CTGTATCTTC TGCTGCAATT GCTGAGGTTG GGCGTGACAA TAAGGAAATT CAGGATGCTA 2101 TAGCTATGGA GGGAGCGATT CCTCCTCTGG TGGCTCTTTT TAAAGGGAAA CAAATTAGTG 2161 TCCAAATGAA AGGTGCAATG GCTGTGGAAT CACTGGCAAG TCACAACGCT CTTATACAGA 2221 AAGCATTTCT GGAAAAATCG TTAACTAAAT ATCTTTTAAA ACTCCTAAAG GCATTTCAAA 2281 TAGATGTTAA GGAACAAGGA GCTGTTGCAC TTTGGGCCTT GGCAGGACAA ACACTAAAAC 2341 AACAAAAATA TATGGCAGAA CAAATTGGAT ACAGCTTTAT AATAAATATG CTTTTGTCAC 2401 CATCAGCTAA AATGCAGTAT GTTGGATATT TGCTTAAGAG CAGGCTATGC ATTAACACTT 2461 TTTGCCTTCA ATTGCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 2521 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 2581 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAGAG AAACTAACTC CCATTCCAAT 2641 TACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t