Construct: ORF TRCN0000477549
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004119.1_s317c1
- Derived from:
- ccsbBroadEn_07603
- DNA Barcode:
- ACGCCGGAACTGGTCTCCTGACCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SPON1 (10418)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477549
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10418 | SPON1 | spondin 1 | NM_006108.4 | 99.9% | 100% | 864A>G |
| 2 | mouse | 233744 | Spon1 | spondin 1, (f-spondin) extr... | NM_145584.2 | 89.7% | 96.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2490
- ORF length:
- 2421
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaggctgtcc ccggcgcccc tgaagctgag ccggactccg gcactgctgg 121 ccctggcgct gcccctggcc gcggcgctgg ccttctccga cgagaccctg gacaaagtgc 181 ccaagtcaga gggctactgc agccgtatcc tgcgcgccca gggcacgcgg cgcgagggct 241 acaccgagtt cagcctccgc gtggagggcg accccgactt ctacaagccg ggaaccagct 301 accgcgtaac actttcagct gctcctccct cctacttcag aggattcaca ttaattgccc 361 tcagagagaa cagagagggt gataaggaag aagaccatgc tgggaccttc cagatcatag 421 acgaagaaga aactcagttt atgagcaatt gccctgttgc agtcactgaa agcactccac 481 ggaggaggac ccggatccag gtgttttgga tagcaccacc agcgggaaca ggctgcgtga 541 ttctgaaggc cagcatcgta caaaaacgca ttatttattt tcaagatgag ggctctctga 601 ccaagaaact ttgtgaacaa gattccacat ttgatggggt gactgacaaa cccatcttag 661 actgctgtgc ctgcggaact gccaagtaca gactcacatt ttatgggaat tggtccgaga 721 agacacaccc aaaggattac cctcgtcggg ccaaccactg gtctgcgatc atcggaggat 781 cccactccaa gaattatgta ctgtgggaat atggaggata tgccagcgaa ggcgtcaaac 841 aagttgcaga attgggctca cccgtgaaaa tggaggaaga aattcgacaa cagagtgatg 901 aggtcctcac cgtcatcaaa gccaaagccc agtggccagc ctggcagcct ctcaacgtga 961 gagcagcacc ttcagctgaa ttttccgtgg acagaacgcg ccatttaatg tccttcctga 1021 ccatgatggg ccctagtccc gactggaacg taggcttatc tgcagaagat ctgtgcacca 1081 aggaatgtgg ctgggtccag aaggtggtgc aagacctgat tccctgggac gctggcaccg 1141 acagcggggt gacctatgag tcacccaaca aacccaccat tccccaggag aaaatccggc 1201 ccctgaccag cctggaccat cctcagagtc ctttctatga cccagagggt gggtccatca 1261 ctcaagtagc cagagttgtc atcgagagaa tcgcacggaa gggtgaacaa tgcaatattg 1321 tacctgacaa tgtcgatgat attgtagctg acctggctcc agaagagaaa gatgaagatg 1381 acacccctga aacctgcatc tactccaact ggtccccatg gtccgcctgc agctcctcca 1441 cctgtgacaa aggcaagagg atgcgacagc gcatgctgaa agcacagctg gacctcagcg 1501 tcccctgccc tgacacccag gacttccagc cctgcatggg ccctggctgc agtgacgaag 1561 acggctccac ctgcaccatg tccgagtgga tcacctggtc gccctgcagc atctcctgcg 1621 gcatgggcat gaggtcccgg gagaggtatg tgaagcagtt cccggaggac ggctccgtgt 1681 gcacgctgcc cactgaggaa acggagaagt gcacggtcaa cgaggagtgc tctcccagca 1741 gctgcctgat gaccgagtgg ggcgagtggg acgagtgcag cgccacctgc ggcatgggca 1801 tgaagaagcg gcaccgcatg atcaagatga accccgcaga tggctccatg tgcaaagccg 1861 agacatcaca ggcagagaag tgcatgatgc cagagtgcca caccatccca tgcttgctgt 1921 ccccatggtc cgagtggagt gactgcagcg tgacctgcgg gaagggcatg cgaacccgac 1981 agcggatgct caagtctctg gcagaacttg gagactgcaa tgaggatctg gagcaggtgg 2041 agaagtgcat gctcccTGAA TGCCCCATTG ACTGTGAGCT CACCGAGTGG TCCCAGTGGT 2101 CGGAATGTAA CAAGTCATGT GGGAAAGGCC ACGTGATTCG AACCCGGATG ATCCAAATGG 2161 AGCCTCAGTT TGGAGGTGCA CCCTGCCCAG AGACTGTGCA GCGAAAAAAG TGCCGCATCC 2221 GAAAATGCCT TCGAAATCCA TCCATCCAAA AGCTACGCTG GAGGGAGGCC CGAGAGAGCC 2281 GGCGGAGTGA GCAGCTGAAG GAAGAGTCTG AAGGGGAGCA GTTCCCAGGT TGTAGGATGC 2341 GCCCATGGAC GGCCTGGTCA GAATGCACCA AACTGTGCGG AGGTGGAATT CAGGAACGTT 2401 ACATGACTGT AAAGAAGAGA TTCAAAAGCT CCCAGTTTAC CAGCTGCAAA GACAAGAAGG 2461 AGATCAGAGC ATGCAATGTT CATCCTTGTT TGCCAACTTT CTTGTACAAA GTGGTTGATA 2521 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 2581 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAACGCC 2641 GGAACTGGTC TCCTGACCTA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 2701 tgaaagatt