Construct: ORF TRCN0000477549

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF004119.1_s317c1
Derived from:
ccsbBroadEn_07603
DNA Barcode:
ACGCCGGAACTGGTCTCCTGACCT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SPON1 (10418)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000477549
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 10418 SPON1 spondin 1 NM_006108.4 99.9% 100% 864A>G
2 mouse 233744 Spon1 spondin 1, (f-spondin) extr... NM_145584.2 89.7% 96.2% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2490
ORF length:
2421
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gaggctgtcc ccggcgcccc tgaagctgag ccggactccg gcactgctgg
121ccctggcgct gcccctggcc gcggcgctgg ccttctccga cgagaccctg gacaaagtgc
181ccaagtcaga gggctactgc agccgtatcc tgcgcgccca gggcacgcgg cgcgagggct
241acaccgagtt cagcctccgc gtggagggcg accccgactt ctacaagccg ggaaccagct
301accgcgtaac actttcagct gctcctccct cctacttcag aggattcaca ttaattgccc
361tcagagagaa cagagagggt gataaggaag aagaccatgc tgggaccttc cagatcatag
421acgaagaaga aactcagttt atgagcaatt gccctgttgc agtcactgaa agcactccac
481ggaggaggac ccggatccag gtgttttgga tagcaccacc agcgggaaca ggctgcgtga
541ttctgaaggc cagcatcgta caaaaacgca ttatttattt tcaagatgag ggctctctga
601ccaagaaact ttgtgaacaa gattccacat ttgatggggt gactgacaaa cccatcttag
661actgctgtgc ctgcggaact gccaagtaca gactcacatt ttatgggaat tggtccgaga
721agacacaccc aaaggattac cctcgtcggg ccaaccactg gtctgcgatc atcggaggat
781cccactccaa gaattatgta ctgtgggaat atggaggata tgccagcgaa ggcgtcaaac
841aagttgcaga attgggctca cccgtgaaaa tggaggaaga aattcgacaa cagagtgatg
901aggtcctcac cgtcatcaaa gccaaagccc agtggccagc ctggcagcct ctcaacgtga
961gagcagcacc ttcagctgaa ttttccgtgg acagaacgcg ccatttaatg tccttcctga
1021ccatgatggg ccctagtccc gactggaacg taggcttatc tgcagaagat ctgtgcacca
1081aggaatgtgg ctgggtccag aaggtggtgc aagacctgat tccctgggac gctggcaccg
1141acagcggggt gacctatgag tcacccaaca aacccaccat tccccaggag aaaatccggc
1201ccctgaccag cctggaccat cctcagagtc ctttctatga cccagagggt gggtccatca
1261ctcaagtagc cagagttgtc atcgagagaa tcgcacggaa gggtgaacaa tgcaatattg
1321tacctgacaa tgtcgatgat attgtagctg acctggctcc agaagagaaa gatgaagatg
1381acacccctga aacctgcatc tactccaact ggtccccatg gtccgcctgc agctcctcca
1441cctgtgacaa aggcaagagg atgcgacagc gcatgctgaa agcacagctg gacctcagcg
1501tcccctgccc tgacacccag gacttccagc cctgcatggg ccctggctgc agtgacgaag
1561acggctccac ctgcaccatg tccgagtgga tcacctggtc gccctgcagc atctcctgcg
1621gcatgggcat gaggtcccgg gagaggtatg tgaagcagtt cccggaggac ggctccgtgt
1681gcacgctgcc cactgaggaa acggagaagt gcacggtcaa cgaggagtgc tctcccagca
1741gctgcctgat gaccgagtgg ggcgagtggg acgagtgcag cgccacctgc ggcatgggca
1801tgaagaagcg gcaccgcatg atcaagatga accccgcaga tggctccatg tgcaaagccg
1861agacatcaca ggcagagaag tgcatgatgc cagagtgcca caccatccca tgcttgctgt
1921ccccatggtc cgagtggagt gactgcagcg tgacctgcgg gaagggcatg cgaacccgac
1981agcggatgct caagtctctg gcagaacttg gagactgcaa tgaggatctg gagcaggtgg
2041agaagtgcat gctcccTGAA TGCCCCATTG ACTGTGAGCT CACCGAGTGG TCCCAGTGGT
2101CGGAATGTAA CAAGTCATGT GGGAAAGGCC ACGTGATTCG AACCCGGATG ATCCAAATGG
2161AGCCTCAGTT TGGAGGTGCA CCCTGCCCAG AGACTGTGCA GCGAAAAAAG TGCCGCATCC
2221GAAAATGCCT TCGAAATCCA TCCATCCAAA AGCTACGCTG GAGGGAGGCC CGAGAGAGCC
2281GGCGGAGTGA GCAGCTGAAG GAAGAGTCTG AAGGGGAGCA GTTCCCAGGT TGTAGGATGC
2341GCCCATGGAC GGCCTGGTCA GAATGCACCA AACTGTGCGG AGGTGGAATT CAGGAACGTT
2401ACATGACTGT AAAGAAGAGA TTCAAAAGCT CCCAGTTTAC CAGCTGCAAA GACAAGAAGG
2461AGATCAGAGC ATGCAATGTT CATCCTTGTT TGCCAACTTT CTTGTACAAA GTGGTTGATA
2521TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC
2581CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAACGCC
2641GGAACTGGTC TCCTGACCTA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg
2701tgaaagatt

Download FASTA (ORF) (Full)