Construct: ORF TRCN0000477551
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016544.1_s317c1
- Derived from:
- ccsbBroadEn_08850
- DNA Barcode:
- TGGGAGGTTGGTTGCGCTCGGTCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RHBDF1 (64285)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477551
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64285 | RHBDF1 | rhomboid 5 homolog 1 | NM_022450.5 | 99.9% | 99.8% | 710A>G;2322A>G |
| 2 | human | 64285 | RHBDF1 | rhomboid 5 homolog 1 | XM_005255494.1 | 99.9% | 99.8% | 710A>G;2322A>G |
| 3 | human | 64285 | RHBDF1 | rhomboid 5 homolog 1 | XM_006720921.1 | 97.3% | 97.2% | 115_183del;779A>G;2391A>G |
| 4 | human | 64285 | RHBDF1 | rhomboid 5 homolog 1 | XM_017023556.1 | 97.3% | 97.2% | 115_183del;779A>G;2391A>G |
| 5 | human | 64285 | RHBDF1 | rhomboid 5 homolog 1 | XM_017023557.1 | 91.1% | 91.1% | (many diffs) |
| 6 | human | 64285 | RHBDF1 | rhomboid 5 homolog 1 | XM_017023558.1 | 66.8% | 65.3% | (many diffs) |
| 7 | human | 64285 | RHBDF1 | rhomboid 5 homolog 1 | XM_005255498.2 | 63% | 63% | 0_1ins948;1374A>G |
| 8 | mouse | 13650 | Rhbdf1 | rhomboid 5 homolog 1 | NM_010117.2 | 88.1% | 95.3% | (many diffs) |
| 9 | mouse | 13650 | Rhbdf1 | rhomboid 5 homolog 1 | XM_006514499.3 | 85.7% | 72.8% | (many diffs) |
| 10 | mouse | 13650 | Rhbdf1 | rhomboid 5 homolog 1 | XM_011243658.2 | 84.6% | 91.5% | (many diffs) |
| 11 | mouse | 13650 | Rhbdf1 | rhomboid 5 homolog 1 | XM_006514494.3 | 82.4% | 70.2% | (many diffs) |
| 12 | mouse | 13650 | Rhbdf1 | rhomboid 5 homolog 1 | XM_006514495.3 | 82.4% | 70.2% | (many diffs) |
| 13 | mouse | 13650 | Rhbdf1 | rhomboid 5 homolog 1 | XM_006514496.3 | 82.4% | 70.2% | (many diffs) |
| 14 | mouse | 13650 | Rhbdf1 | rhomboid 5 homolog 1 | XM_011243657.2 | 78.8% | 66.7% | (many diffs) |
| 15 | mouse | 13650 | Rhbdf1 | rhomboid 5 homolog 1 | XR_872202.1 | 67.9% | (many diffs) | |
| 16 | mouse | 13650 | Rhbdf1 | rhomboid 5 homolog 1 | NM_001291818.1 | 66% | 71.8% | (many diffs) |
| 17 | mouse | 13650 | Rhbdf1 | rhomboid 5 homolog 1 | XM_006514501.3 | 64.2% | 51.8% | (many diffs) |
| 18 | mouse | 13650 | Rhbdf1 | rhomboid 5 homolog 1 | XM_006514503.2 | 58.9% | 46.5% | (many diffs) |
| 19 | mouse | 13650 | Rhbdf1 | rhomboid 5 homolog 1 | XR_001779873.1 | 46.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2631
- ORF length:
- 2565
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag tgaggcccgc agggacagca cgagcagcct gcagcgcaag aagccaccct 121 ggctaaagct ggacattccc tctgcggtgc ccctgacggc agaagagccc agcttcctgc 181 agcccctgag gcgacaggct ttcctgagga gtgtgagtat gccagccgag acagcccaca 241 tctcttcacc ccaccatgag ctccggcggc cggtgctgca acgccagacg tccatcacac 301 agaccatccg cagggggacc gccgactggt ttggagtgag caaggacagt gacagcaccc 361 agaaatggca gcgcaagagc atccgtcact gcagccagcg ctacgggaag ctgaagcccc 421 aggtcctccg ggagctggac ctgcccagcc aggacaacgt gtcgctgacc agcaccgaga 481 cgccaccccc actctacgtg gggccatgcc agctgggcat gcagaagatc atagaccccc 541 tggcccgtgg ccgtgccttc cgtgtggcag atgacactgc ggaaggcctg agtgccccac 601 acactcccgt cacgccgggt gctgcctccc tctgctcctt ctccagctcc cgctcaggtt 661 tccaccggct cccgcggcgg cgcaagcgag agtcggtggc caagatgagc ttccgggcgg 721 ccgcagcgct gatgaaaggc cgctccgtta gggatggcac ctttcgccgg gcacggcgtc 781 gaagcttcac tccagctagc tttctggagg aggacacaac tgatttcccc gatgagctgg 841 acacatcctt ctttgcccgg gaaggtatcc tccatgaaga gctgtccaca tacccggatg 901 aagttttcga gtccccatcg gaggcagcgc taaaggactg ggagaaggca ccggagcagg 961 cggacctcac cggcggggcc ctggaccgca gcgagcttga gcgcagccac ctgatgctgc 1021 ccttggagcg aggctggcgg aagcagaagg agggcgccgc agccccgcag cccaaggtgc 1081 ggctccgaca ggaggtggtg agcaccgcgg ggccgcgacg gggccagcgt atcgcggtgc 1141 cggtgcgcaa gctcttcgcc cgggagaagc ggccgtatgg gctgggcatg gtgggacggc 1201 tcaccaaccg cacctaccgc aagcgcatcg acagcttcgt caagcgccag atcgaggaca 1261 tggacgacca caggcccttc ttcacctact ggcttacctt cgtgcactcg ctcgtcacca 1321 tcctagccgt gtgcatctat ggcatcgcgc ccgtgggctt ctcgcagcat gagacggtgg 1381 actcggtgct gcggaaccgc ggggtctacg agaacgtcaa gtacgtgcag caggagaact 1441 tctggatcgg gcccagctcg gaggccctca tccacctggg cgccaagttt tcgccctgca 1501 tgcgccagga cccgcaggtg cacagcttca ttcgctcggc gcgcgagcgc gagaagcact 1561 ccgcctgctg cgtgcgcaac gacaggtcgg gctgcgtgca gacctcggag gaggagtgct 1621 cgtccacgct ggcagtgtgg gtgaagtggc ccatccatcc cagcgcccca gagcttgcgg 1681 gccacaagag acagtttggc tctgtctgcc accaggatcc cagggtgtgt gatgagccct 1741 cctccgaaga ccctcatgag tggccagaag acatcaccaa gtggccgatc tgcaccaaaa 1801 acagcgctgg gaaccacacc aaccatcccc acatggactg tgtcatcaca ggacggccct 1861 gctgcattgg caccaagggc aggtgtgaga tcacctcccg ggagtactgt gacttcatga 1921 ggggctactt ccatgaggag gccacgctct gctctcaggt gcactgcatg gatgatgtgt 1981 gtgggctcct gccttttctc aaccccgagg tgcctgacca gttctaccgc ctgtggctat 2041 ccctcttcct gcacgccggg atcttgcact gcctggtgtc catctgcttc cagatgactg 2101 tcctgcggga cctggagaag ctggcaggct ggcaccgcat agccatcatc tacctgctga 2161 gtggtgtcac cggcaacctg gccagtgcca tcttcctgcc ataccgagca gaggtgggtc 2221 ctgctggctc ccagttcggc atcctggcct gcctcttcgt ggagctcttc cagagctggc 2281 agatcctggc gcggccctgg cgtgccttct tcaagctgct ggctgtggtg ctcttcctct 2341 tcacctttGG GCTGCTGCCG TGGATTGACA ACTTTGCCCA CATCTCGGGG TTCATCAGTG 2401 GCCTCTTCCT CTCCTTCGCC TTCTTGCCCT ACATCAGCTT TGGCAAGTTC GACCTGTACC 2461 GGAAACGCTG CCAGATCATC ATCTTTCAGG TGGTCTTCCT GGGCCTCCTG GCTGGCCTGG 2521 TGGTCCTCTT CTACGTCTAT CCTGTCCGCT GTGAGTGGTG TGAGTTCCTC ACCTGCATCC 2581 CCTTCACTGA CAAGTTCTGT GAGAAGTACG AACTGGACGC TCAGCTCCAC TGCCCAACTT 2641 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 2701 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 2761 CTTGTGGAAA GGACGATGGG AGGTTGGTTG CGCTCGGTCT ACGCGTTAAG TCgacaatca 2821 acctctggat tacaaaattt gtgaaagatt