Construct: ORF TRCN0000477556
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012209.1_s317c1
- Derived from:
- ccsbBroadEn_07969
- DNA Barcode:
- AGTACAGGTGTTCAGATGACTCCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FAM98A (25940)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477556
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 25940 | FAM98A | family with sequence simila... | NM_015475.5 | 99.9% | 99.8% | 1339A>G |
| 2 | human | 25940 | FAM98A | family with sequence simila... | NM_001304538.1 | 62.2% | 62.1% | 0_1ins585;754A>G |
| 3 | mouse | 72722 | Fam98a | family with sequence simila... | NM_133747.2 | 91.2% | 94.9% | (many diffs) |
| 4 | mouse | 72722 | Fam98a | family with sequence simila... | XM_017317659.1 | 55.6% | 58.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1623
- ORF length:
- 1554
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggagtgtgac ctcatggaga ctgacatctt ggagtcgttg gaagatctag 121 gttacaaggg cccattgttg gaagatggag cgctctctca ggcagtctct gctggagcca 181 gttcccccga gtttaccaaa ctctgtgctt ggctggtgtc tgaattaaga gtgctctgta 241 aactagagga aaacgtgcaa gcaactaaca gtccgagtga agctgaagaa ttccagcttg 301 aggtgagtgg gctactaggg gagatgaact gcccgtatct ttcactgaca tctggggatg 361 tgaccaagcg ccttctcatt cagaagaact gcctcctctt gctcacatac ctcatctcag 421 aactagaagc tgccagaatg ctctgtgtga atgctcctcc aaaaaaagct caagaaggag 481 gcggtagtga ggtctttcaa gagttgaaag gcatatgtat tgctctagga atgtccaaac 541 ctccagccaa tataactatg ttccaattct tcagcgggat tgaaaaaaaa ttaaaggaaa 601 cattagcaaa agttccacct aatcatgtgg gaaagccttt actgaagaag ccaatgggac 661 cagcccactg ggaaaagata gaagcaatta accaagccat agccaatgaa tatgaagtcc 721 ggagaaagct gctaataaaa cgtttggatg tcactgtaca atcctttggc tggtctgaca 781 gagctaagag ccagacagaa aaattagcca aggtttacca gccgaaacgt tcagtcttat 841 cccctaaaac tactatttct gttgcccatc ttttggctgc aaggcaggac ttgtcaaaga 901 ttttaaggac aagcagcggc tctataagag aaaagactgc ctgtgccatc aataaggtgt 961 tgatgggcag ggtgcctgac agaggtggta gacccaatga aatcgaacct ccacccccag 1021 agatgccacc gtggcagaag aggcaagatg gcccccagca gcaaacagga ggccgaggag 1081 gagggagagg tggctatgaa cattcctcat acggaggacg aggaggtcat gaacaaggag 1141 gcgggagagg tggacgtggt ggctatgacc atggtggccg agggggagga agaggaaata 1201 agcatcaagg aggctggaca gatggaggga gtggtggagg aggtggctac caagatggtg 1261 gttatcgaga ttcaggtttc cagccaggtg gctatcatgg tggccacagc agtggtggct 1321 atcaaggcgg aggttatggt ggcttccaaa catcttcttc atatacagga agtggatacc 1381 agggtggtgg ctaccagcag gacaatggat accaagatgg cgggcaccat ggtgATCGTG 1441 GTGGTGGTCG TGGTGGGCGA GGTGGTCGTG GAGGCCGAGG TGGTCGTGCA GGCCAGGGAG 1501 GAGGCTGGGG AGGAAGAGGG AGCCAGAATT ATCACCAAGG GGGTCAATTT GAACAGCATT 1561 TCCAGCATGG AGGTTATCAG TATAATCATT CTGGATTTGG ACAGGGAAGA CATTACACTA 1621 GTTTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1681 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1741 GGCTTTATAT ATCTTGTGGA AAGGACGAAG TACAGGTGTT CAGATGACTC CAACGCGTTA 1801 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt