Transcript: Mouse NM_133747.2

Mus musculus family with sequence similarity 98, member A (Fam98a), mRNA.

Source:
NCBI, updated 2017-05-20
Taxon:
Mus musculus (mouse)
Gene:
Fam98a (72722)
Length:
2817
CDS:
154..1701

Additional Resources:

NCBI RefSeq record:
NM_133747.2
NBCI Gene record:
Fam98a (72722)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_133747.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000264360 CGCTCACTCTTGTCGCCTAAA pLKO_005 913 CDS 100% 10.800 15.120 N Fam98a n/a
2 TRCN0000264358 GCCTTTAGTTGACCGGTAAAC pLKO_005 1954 3UTR 100% 10.800 15.120 N Fam98a n/a
3 TRCN0000181009 GAAGCTGCTAATCAAACGCTT pLKO.1 810 CDS 100% 2.640 3.696 N Fam98a n/a
4 TRCN0000147539 GAGGTCTTTCAAGAGTTGAAA pLKO.1 574 CDS 100% 0.563 0.788 N FAM98A n/a
5 TRCN0000278967 GAGGTCTTTCAAGAGTTGAAA pLKO_005 574 CDS 100% 0.563 0.788 N FAM98A n/a
6 TRCN0000264359 CCAAACCTCCAGCCAATATAA pLKO_005 620 CDS 100% 15.000 10.500 N Fam98a n/a
7 TRCN0000297645 CCAAACCTCCAGCCAATATAA pLKO_005 620 CDS 100% 15.000 10.500 N FAM98A n/a
8 TRCN0000374721 TTGGACAAGGAAGACATTATA pLKO_005 1673 CDS 100% 15.000 10.500 N Fam98a n/a
9 TRCN0000181179 CCAAGCCATAGCCAATGAATA pLKO.1 777 CDS 100% 13.200 9.240 N Fam98a n/a
10 TRCN0000374720 TTGCTAGAGCTCAAGTAATAG pLKO_005 1721 3UTR 100% 13.200 9.240 N Fam98a n/a
11 TRCN0000374657 AGGCTATCAGTATAGTCATTC pLKO_005 1647 CDS 100% 10.800 7.560 N Fam98a n/a
12 TRCN0000283010 GGACGGTGCTTATCGAGATTC pLKO_005 1335 CDS 100% 10.800 7.560 N Fam98a n/a
13 TRCN0000147316 GCACATTCAGTAGCCTTATTT pLKO.1 2271 3UTR 100% 15.000 21.000 N FAM98A n/a
14 TRCN0000278968 GCACATTCAGTAGCCTTATTT pLKO_005 2271 3UTR 100% 15.000 21.000 N FAM98A n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_133747.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07968 pDONR223 100% 91.3% 95.1% None (many diffs) n/a
2 ccsbBroad304_07968 pLX_304 0% 91.3% 95.1% V5 (many diffs) n/a
3 TRCN0000477620 TCAAGTGGCAATTGTATAACACGT pLX_317 28.7% 91.3% 95.1% V5 (many diffs) n/a
4 ccsbBroadEn_07969 pDONR223 100% 91.2% 94.9% None (many diffs) n/a
5 ccsbBroad304_07969 pLX_304 0% 91.2% 94.9% V5 (many diffs) n/a
6 TRCN0000477556 AGTACAGGTGTTCAGATGACTCCA pLX_317 12.6% 91.2% 94.9% V5 (many diffs) n/a
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