Construct: ORF TRCN0000477562
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001374.1_s317c1
- Derived from:
- ccsbBroadEn_01127
- DNA Barcode:
- AAATCAGATCTAGTCTCTTGGACG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- OTC (5009)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477562
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5009 | OTC | ornithine carbamoyltransferase | NM_000531.6 | 100% | 100% | |
| 2 | human | 5009 | OTC | ornithine carbamoyltransferase | XM_017029556.1 | 84.2% | 83.6% | (many diffs) |
| 3 | mouse | 18416 | Otc | ornithine transcarbamylase | NM_008769.4 | 89.1% | 92.6% | (many diffs) |
| 4 | mouse | 18416 | Otc | ornithine transcarbamylase | XM_011247449.2 | 85.8% | 88.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1131
- ORF length:
- 1062
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gctgtttaat ctgaggatcc tgttaaacaa tgcagctttt agaaatggtc 121 acaacttcat ggttcgaaat tttcggtgtg gacaaccact acaaaataaa gtgcagctga 181 agggccgtga ccttctcact ctaaaaaact ttaccggaga agaaattaaa tatatgctat 241 ggctatcagc agatctgaaa tttaggataa aacagaaagg agagtatttg cctttattgc 301 aagggaagtc cttaggcatg atttttgaga aaagaagtac tcgaacaaga ttgtctacag 361 aaacaggctt tgcacttctg ggaggacatc cttgttttct taccacacaa gatattcatt 421 tgggtgtgaa tgaaagtctc acggacacgg cccgtgtatt gtctagcatg gcagatgcag 481 tattggctcg agtgtataaa caatcagatt tggacaccct ggctaaagaa gcatccatcc 541 caattatcaa tgggctgtca gatttgtacc atcctatcca gatcctggct gattacctca 601 cgctccagga acactatagc tctctgaaag gtcttaccct cagctggatc ggggatggga 661 acaatatcct gcactccatc atgatgagcg cagcgaaatt cggaatgcac cttcaggcag 721 cTACTCCAAA GGGTTATGAG CCGGATGCTA GTGTAACCAA GTTGGCAGAG CAGTATGCCA 781 AAGAGAATGG TACCAAGCTG TTGCTGACAA ATGATCCATT GGAAGCAGCG CATGGAGGCA 841 ATGTATTAAT TACAGACACT TGGATAAGCA TGGGACAAGA AGAGGAGAAG AAAAAGCGGC 901 TCCAGGCTTT CCAAGGTTAC CAGGTTACAA TGAAGACTGC TAAAGTTGCT GCCTCTGACT 961 GGACATTTTT ACACTGCTTG CCCAGAAAGC CAGAAGAAGT GGATGATGAA GTCTTTTATT 1021 CTCCTCGATC ACTAGTGTTC CCAGAGGCAG AAAACAGAAA GTGGACAATC ATGGCTGTCA 1081 TGGTGTCCCT GCTGACAGAT TACTCACCTC AGCTCCAGAA GCCTAAATTT TTGCCAACTT 1141 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1201 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1261 CTTGTGGAAA GGACGAAAAT CAGATCTAGT CTCTTGGACG ACGCGTTAAG TCgacaatca 1321 acctctggat tacaaaattt gtgaaagatt