Construct: ORF TRCN0000477622
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004343.1_s317c1
- Derived from:
- ccsbBroadEn_15920
- DNA Barcode:
- CTAGAGAGATTTAGCCCGCTCTCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KLHL7 (55975)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477622
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55975 | KLHL7 | kelch like family member 7 | NM_001172428.1 | 83% | 75.4% | (many diffs) |
| 2 | human | 55975 | KLHL7 | kelch like family member 7 | XM_017012441.2 | 45.7% | 42.5% | (many diffs) |
| 3 | human | 55975 | KLHL7 | kelch like family member 7 | XM_017012440.2 | 41% | 34.6% | (many diffs) |
| 4 | human | 55975 | KLHL7 | kelch like family member 7 | XM_006715757.4 | 39.9% | 36.1% | (many diffs) |
| 5 | human | 55975 | KLHL7 | kelch like family member 7 | NR_033329.1 | 37% | 1_490del;776C>T;923_1163del | |
| 6 | human | 55975 | KLHL7 | kelch like family member 7 | XM_017012439.2 | 24.6% | 23% | (many diffs) |
| 7 | human | 55975 | KLHL7 | kelch like family member 7 | XM_006715754.3 | 24.3% | 24% | (many diffs) |
| 8 | human | 55975 | KLHL7 | kelch like family member 7 | XM_006715755.3 | 24.3% | 24% | (many diffs) |
| 9 | human | 55975 | KLHL7 | kelch like family member 7 | NM_001031710.3 | 22.8% | 19.4% | (many diffs) |
| 10 | human | 55975 | KLHL7 | kelch like family member 7 | XM_006715753.3 | 22.6% | 20.5% | (many diffs) |
| 11 | human | 55975 | KLHL7 | kelch like family member 7 | NM_018846.5 | 20% | 18.4% | (many diffs) |
| 12 | human | 55975 | KLHL7 | kelch like family member 7 | XM_006715756.3 | 19.8% | 19.5% | (many diffs) |
| 13 | human | 55975 | KLHL7 | kelch like family member 7 | NR_033328.2 | 7.2% | (many diffs) | |
| 14 | mouse | 52323 | Klhl7 | kelch-like 7 | NM_001161800.1 | 25.6% | 24% | (many diffs) |
| 15 | mouse | 52323 | Klhl7 | kelch-like 7 | NM_026448.3 | 20.8% | 19.4% | (many diffs) |
| 16 | mouse | 52323 | Klhl7 | kelch-like 7 | XM_006535753.3 | 15.2% | 14.4% | (many diffs) |
| 17 | mouse | 52323 | Klhl7 | kelch-like 7 | XR_880834.2 | 6.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 498
- ORF length:
- 432
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct gggagggact gattgcagaa ccTTCTTGAC AAGCCACATA AATCTAAAGA 121 AAACGTTGTG TGACGTGATC CTCATGGTCC AGGAAAGAAA GATACCTGCT CATCGTGTTG 181 TTCTTGCTGC AGCCAGTCAT TTTTTTAACT TAATGTTCAC AACTAACATG CTTGAATCAA 241 AGTCCTTTGA AGTAGAACTC AAAGATGCTG AACCTGATAT TATTGAACAA CTGGTGGAAT 301 TTGCTTATAC TGCTAGAATT TCCGTGAATA GCAACAATGT TCAGTCTTTG TTGGATGCAG 361 CAAACCAATA TCAGATTGAA CCTGTGAAGA AAATGTGTGT TGATTTTTTG AAAGAACAAG 421 TTGATGCTTC AAATTGTCTT GGAGAAGCAG AAAAAGTTGA TCAGAGCCTT CCAGAGTGTG 481 GTATGCTTTT CACTGTGTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 541 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 601 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGACTAGAGA GATTTAGCCC 661 GCTCTCAACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt