Construct: ORF TRCN0000477625
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016836.1_s317c1
- Derived from:
- ccsbBroadEn_09683
- DNA Barcode:
- TGGAAACATACTTCTGCTCTCCCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CCDC13 (152206)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477625
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 152206 | CCDC13 | coiled-coil domain containi... | NM_144719.4 | 99.9% | 99.8% | 1124A>T |
| 2 | human | 152206 | CCDC13 | coiled-coil domain containi... | XM_005264898.3 | 94.2% | 94.1% | 1124A>T;1594_1595ins123 |
| 3 | human | 152206 | CCDC13 | coiled-coil domain containi... | XM_011533418.2 | 90.3% | 90.2% | 1124A>T;1509_1510ins207 |
| 4 | human | 152206 | CCDC13 | coiled-coil domain containi... | XM_011533419.2 | 69.1% | 69% | 0_1ins660;464A>T |
| 5 | human | 152206 | CCDC13 | coiled-coil domain containi... | XM_017005781.1 | 69.1% | 69% | 0_1ins660;464A>T |
| 6 | human | 152206 | CCDC13 | coiled-coil domain containi... | XM_011533420.2 | 66% | 64.4% | (many diffs) |
| 7 | human | 152206 | CCDC13 | coiled-coil domain containi... | XM_005264899.4 | 64.5% | 63.9% | (many diffs) |
| 8 | human | 152206 | CCDC13 | coiled-coil domain containi... | XM_011533421.2 | 64.5% | 63.6% | (many diffs) |
| 9 | human | 152206 | CCDC13 | coiled-coil domain containi... | XR_940385.1 | 31.7% | 1_84del;1208A>T;2230_6754del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 65
- ORF end:
- 2210
- ORF length:
- 2145
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttgcatggca gcagatgaaa gctcacagaa cactttgcgg ctccagttca aggcaatgca 121 ggagatgcag cacaaacggt tacagaagca gatggagaaa aagagggaaa aagaactgag 181 cctcaaaagc agagctgacg accaagagga gcccttggag gtttcagatg gcctcagcct 241 tctccacgca ggggagccaa actcgaaaaa tagctttgag aagagggtgc ttgaagatga 301 gattgaacac cttcgaaatg agctcaggga aacggtggac gagaacgggc gattgtataa 361 gctgctgaag gaaagggact ttgaaatcaa acacctcaaa aagaaaatag aagaggacag 421 atttgccttc acagggacag ccggtgtagc cggagacgtg gtcgccacca aaattgtgga 481 gctatcaaaa aagaaccggc tgttgatggc agaatcagag ggtgcaaaaa ccagggtgaa 541 gcagctgacc aatcgcatcc aggagctgga gcgggaactg cagacagccc tgaccaggct 601 gtcagccaag ggggccaccg acgcaggagc caagccaccg agggcccaga tgggagacag 661 agcattgctg gagaccccag aggtgaaggc cctgcaggac aggctggtgg ccaccaactt 721 gaagatgagt gacctccgaa accagatcca gtctgtgaag caggagctgc ggatggcaca 781 gaaggttttg gccagagagg ttggggaaga catcaacgtt cagcagctcc tatcttcgcc 841 agggacctgg aggggtcggg ctcaacaaat tcttgttttg cagagcaagg ttcaagagct 901 tgagaagcaa ttgggccagg cccggagcca gtctgcggga acagccagtg atgagttgtc 961 tgtctatcca gacccaagga agctgtcggc acaggagaaa aacctgctga ggatccgcag 1021 cctggaaagg gaaaaacagg aaggcttgga gaaacttgcc agtgaacggg atgtcctcca 1081 gagagagctt gaagagctaa aaaagaagtt cgagggcatg aggtctcgga acaagctgct 1141 gtcaagtgag atgaagaccc tcaagagtca gatgggaacc ctggtggtga agggccggca 1201 tgatgacgag ctcatcgacg ccctcatgga ccagctgaag cagctacagg agatcctagg 1261 cagcctgagt ctgcaggagg agaaaacgcg agtgtcccag caccacctgg accagcaact 1321 gaacagcgag gctcagcgga gcaacagcct agtcgcccag ctgcaggcca tggtagctga 1381 gcgggaggcc aaagtgcgac agctggagat ggagatcgga caactcaatg tgcactatct 1441 tcggaataaa ggagtgggtg aggggtccag tggccgcgag gtcagccctg cctataccca 1501 gttcctggag gacccaggcc tgaccaagtc cccggcctca gcaggcgatc atgttggcag 1561 gcttggatct tctcgctctg tgaccagcct gggccacaca ctggtggaat ctgctctcac 1621 gaggccttcc ctgcccagtc cccacaggac ctcacctagg ttctcggact ccccagaaca 1681 aaaaggctgg caggcacaag tgtcagagat caaggccctc tggcaggctg ccgaggtgga 1741 gcgtgaccgg ctcaccgagt ttgtcactgt cctgcagaaa cgggtggagg agagcaacag 1801 caagctcctG GAGTCAGAGA GGAAGCTGCA GGAGGAGCGA CACCGCACCG TGGTGCTGGA 1861 GCAACATCTG GAGAAGATAC GCCTGGAGCC AGGGAAGGCA TCAGCCTCCC AGAGAGCAGC 1921 TCCCAGGACC AAAACAGGTC TGCCCACCTC TAACAACAGG CACAACCCAA CCGGGAGCGA 1981 GAAGAAGGAC CCATCCTTTG CCCAGCTCTC CGATGTGCCC GTGGAATCCC AAATGGAAGA 2041 GCTGACAACC AGGCTGGCCA TCCAGGTGGA GGAGAATGAA ATGCTAAAGG CTGCCCTGGG 2101 CAGTGCCCTG CGGGGAAAGG AGGAGGACTT CCGGATGTAC CATGAGATCC TGGGCCAGGT 2161 GAAAAGTGTC TTCCTGCAGG CCCTGCGGCA GCAGAAGACA GGCAAGCAAT ACCCAACTTT 2221 CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC 2281 TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC 2341 TTGTGGAAAG GACGATGGAA ACATACTTCT GCTCTCCCAA CGCGTTAAGT Cgacaatcaa 2401 cctctggatt acaaaatttg tgaaagatt