Construct: ORF TRCN0000477625

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF016836.1_s317c1
Derived from:
ccsbBroadEn_09683
DNA Barcode:
TGGAAACATACTTCTGCTCTCCCA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CCDC13 (152206)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000477625
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 152206 CCDC13 coiled-coil domain containi... NM_144719.4 99.9% 99.8% 1124A>T
2 human 152206 CCDC13 coiled-coil domain containi... XM_005264898.3 94.2% 94.1% 1124A>T;1594_1595ins123
3 human 152206 CCDC13 coiled-coil domain containi... XM_011533418.2 90.3% 90.2% 1124A>T;1509_1510ins207
4 human 152206 CCDC13 coiled-coil domain containi... XM_011533419.2 69.1% 69% 0_1ins660;464A>T
5 human 152206 CCDC13 coiled-coil domain containi... XM_017005781.1 69.1% 69% 0_1ins660;464A>T
6 human 152206 CCDC13 coiled-coil domain containi... XM_011533420.2 66% 64.4% (many diffs)
7 human 152206 CCDC13 coiled-coil domain containi... XM_005264899.4 64.5% 63.9% (many diffs)
8 human 152206 CCDC13 coiled-coil domain containi... XM_011533421.2 64.5% 63.6% (many diffs)
9 human 152206 CCDC13 coiled-coil domain containi... XR_940385.1 31.7% 1_84del;1208A>T;2230_6754del
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
65
ORF end:
2210
ORF length:
2145
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttgcatggca gcagatgaaa gctcacagaa cactttgcgg ctccagttca aggcaatgca
121ggagatgcag cacaaacggt tacagaagca gatggagaaa aagagggaaa aagaactgag
181cctcaaaagc agagctgacg accaagagga gcccttggag gtttcagatg gcctcagcct
241tctccacgca ggggagccaa actcgaaaaa tagctttgag aagagggtgc ttgaagatga
301gattgaacac cttcgaaatg agctcaggga aacggtggac gagaacgggc gattgtataa
361gctgctgaag gaaagggact ttgaaatcaa acacctcaaa aagaaaatag aagaggacag
421atttgccttc acagggacag ccggtgtagc cggagacgtg gtcgccacca aaattgtgga
481gctatcaaaa aagaaccggc tgttgatggc agaatcagag ggtgcaaaaa ccagggtgaa
541gcagctgacc aatcgcatcc aggagctgga gcgggaactg cagacagccc tgaccaggct
601gtcagccaag ggggccaccg acgcaggagc caagccaccg agggcccaga tgggagacag
661agcattgctg gagaccccag aggtgaaggc cctgcaggac aggctggtgg ccaccaactt
721gaagatgagt gacctccgaa accagatcca gtctgtgaag caggagctgc ggatggcaca
781gaaggttttg gccagagagg ttggggaaga catcaacgtt cagcagctcc tatcttcgcc
841agggacctgg aggggtcggg ctcaacaaat tcttgttttg cagagcaagg ttcaagagct
901tgagaagcaa ttgggccagg cccggagcca gtctgcggga acagccagtg atgagttgtc
961tgtctatcca gacccaagga agctgtcggc acaggagaaa aacctgctga ggatccgcag
1021cctggaaagg gaaaaacagg aaggcttgga gaaacttgcc agtgaacggg atgtcctcca
1081gagagagctt gaagagctaa aaaagaagtt cgagggcatg aggtctcgga acaagctgct
1141gtcaagtgag atgaagaccc tcaagagtca gatgggaacc ctggtggtga agggccggca
1201tgatgacgag ctcatcgacg ccctcatgga ccagctgaag cagctacagg agatcctagg
1261cagcctgagt ctgcaggagg agaaaacgcg agtgtcccag caccacctgg accagcaact
1321gaacagcgag gctcagcgga gcaacagcct agtcgcccag ctgcaggcca tggtagctga
1381gcgggaggcc aaagtgcgac agctggagat ggagatcgga caactcaatg tgcactatct
1441tcggaataaa ggagtgggtg aggggtccag tggccgcgag gtcagccctg cctataccca
1501gttcctggag gacccaggcc tgaccaagtc cccggcctca gcaggcgatc atgttggcag
1561gcttggatct tctcgctctg tgaccagcct gggccacaca ctggtggaat ctgctctcac
1621gaggccttcc ctgcccagtc cccacaggac ctcacctagg ttctcggact ccccagaaca
1681aaaaggctgg caggcacaag tgtcagagat caaggccctc tggcaggctg ccgaggtgga
1741gcgtgaccgg ctcaccgagt ttgtcactgt cctgcagaaa cgggtggagg agagcaacag
1801caagctcctG GAGTCAGAGA GGAAGCTGCA GGAGGAGCGA CACCGCACCG TGGTGCTGGA
1861GCAACATCTG GAGAAGATAC GCCTGGAGCC AGGGAAGGCA TCAGCCTCCC AGAGAGCAGC
1921TCCCAGGACC AAAACAGGTC TGCCCACCTC TAACAACAGG CACAACCCAA CCGGGAGCGA
1981GAAGAAGGAC CCATCCTTTG CCCAGCTCTC CGATGTGCCC GTGGAATCCC AAATGGAAGA
2041GCTGACAACC AGGCTGGCCA TCCAGGTGGA GGAGAATGAA ATGCTAAAGG CTGCCCTGGG
2101CAGTGCCCTG CGGGGAAAGG AGGAGGACTT CCGGATGTAC CATGAGATCC TGGGCCAGGT
2161GAAAAGTGTC TTCCTGCAGG CCCTGCGGCA GCAGAAGACA GGCAAGCAAT ACCCAACTTT
2221CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC
2281TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC
2341TTGTGGAAAG GACGATGGAA ACATACTTCT GCTCTCCCAA CGCGTTAAGT Cgacaatcaa
2401cctctggatt acaaaatttg tgaaagatt

Download FASTA (ORF) (Full)