Construct: ORF TRCN0000477647
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002688.1_s317c1
- Derived from:
- ccsbBroadEn_04804
- DNA Barcode:
- TTATAATTGTTTCACCTCCCCCGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MICOS13 (125988)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477647
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 125988 | MICOS13 | mitochondrial contact site ... | NM_205767.2 | 100% | 100% | |
| 2 | human | 125988 | MICOS13 | mitochondrial contact site ... | NM_001308240.1 | 81.9% | 79.2% | (many diffs) |
| 3 | human | 125988 | MICOS13 | mitochondrial contact site ... | NM_001365761.1 | 81.9% | 79.2% | (many diffs) |
| 4 | human | 125988 | MICOS13 | mitochondrial contact site ... | XM_011527675.2 | 60.4% | 57.8% | (many diffs) |
| 5 | mouse | 224904 | 2410015M20Rik | RIKEN cDNA 2410015M20 gene | NM_153152.3 | 84.3% | 86.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 93
- ORF end:
- 447
- ORF length:
- 354
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA CAATGGTGGC CCGGGTGTGG TCGCTGATGA 121 GGTTCCTCAT CAAGGGAAGT GTGGCTGGGG GCGCCGTCTA CCTGGTGTAC GACCAGGAGC 181 TGCTGGGGCC CAGCGACAAG AGCCAGGCAG CCCTACAGAA GGCTGGGGAG GTGGTCCCCC 241 CCGCCATGTA CCAGTTCAGC CAGTACGTGT GTCAGCAGAC AGGCCTGCAG ATACCCCAGC 301 TCCCAGCCCC TCCAAAGATT TACTTTCCCA TCCGTGACTC CTGGAATGCA GGCATCATGA 361 CGGTGATGTC AGCTCTGTCG GTGGCCCCCT CCAAGGCCCG CGAGTACTCC AAGGAGGGCT 421 GGGAGTATGT GAAGGCGCGC ACCAAGTTGC CAACTTTCTT GTACAAAGTG GTTGATATCG 481 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 541 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GATTATAATT 601 GTTTCACCTC CCCCGTACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 661 aagatt