Construct: ORF TRCN0000477699
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016981.1_s317c1
- Derived from:
- ccsbBroadEn_08377
- DNA Barcode:
- CCTCCTCACAATAGAAAGGAAAAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TAF7L (54457)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477699
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54457 | TAF7L | TATA-box binding protein as... | NM_024885.4 | 99.9% | 99.7% | 101T>C |
| 2 | human | 54457 | TAF7L | TATA-box binding protein as... | XM_006724664.1 | 99.7% | 99.5% | 101T>C;948_950delAGT |
| 3 | human | 54457 | TAF7L | TATA-box binding protein as... | NM_001168474.2 | 81.3% | 81.3% | 0_1ins258 |
| 4 | human | 54457 | TAF7L | TATA-box binding protein as... | XM_005262152.1 | 81.2% | 81.2% | 0_1ins258;690_692delAGT |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1452
- ORF length:
- 1386
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gtgccccgag ggacagctcc ccatttcttc agaaaatgat tcaacaccaa 121 cagtttcaac atcggaagta actagccaac aagaacccca aattcctgtc gaccgtgggt 181 ctgaaaccac ctatgaaagc agcgccgaca ttgctggcga cgaaggcacc cagattcctg 241 ccgacgagga cactcaaacg gatgcggaca gcagcgccca ggctgctgcc caggccccag 301 aaaacttcca ggaagggaaa gacatgagtg aaagccagga tgaagttcct gatgaagttg 361 agaaccagtt tatattgcgt ctgcctctgg aacatgcttg tactgtcagg aacctagcac 421 gttctcaaag tgtcaagatg aaggataaac taaaaattga cttattgcct gatgggcgcc 481 atgcagttgt tgaagtagaa gatgtcccac tagctgctaa gctggttgac ttgccttgtg 541 ttattgaaag cctgagaacg cttgataaaa aaacctttta taaaacagca gacatttctc 601 agatgcttgt gtgcactgct gatggtgata tccacctttc tccagaagaa ccagctgcct 661 ctactgatcc taatatagtc aggaaaaaag aaagggggag agaagaaaaa tgtgtctgga 721 agcatggcat tacgccacca cttaagaatg tcagaaagaa aaggttccgg aaaacacaaa 781 aaaaggtccc tgatgtcaaa gaaatggaaa aaagcagctt tactgagtac attgaatctc 841 cagacgtgga aaatgaagta aagagactgc tgcgttcgga tgctgaagcc gtaagtaccc 901 gttgggaagt cattgctgaa gatggaacca aggaaataga aagtcaaggc tccatcccag 961 gatttttgat atcctcggga atgagcagcc acaagcaggg tcatacctcg tcagaatatg 1021 atatgcttcg ggagatgttc agtgattcta gaagtaaCAA TGATGATGAT GAGGATGAGG 1081 ATGATGAAGA TGAGGATGAG GATGAGGATG AGGATGAAGA TGAAGACAAA GAAGAGGAGG 1141 AGGAAGATTG TTCTGAAGAG TATCTGGAAA GGCAGCTGCA GGCCGAGTTT ATTGAATCTG 1201 GCCAGTATAG GGCAAATGAA GGTACCAGTT CAATAGTCAT GGAAATTCAG AAGCAGATTG 1261 AGAAAAAGGA GAAAAAGCTC CATAAGATTC AGAATAAAGC ACAAAGACAG AAGGATCTCA 1321 TCATGAAAGT GGAAAACCTG ACACTCAAGA ATCATTTTCA GTCTGTGCTG GAGCAGCTTG 1381 AGTTACAGGA AAAACAAAAA AATGAGAAGC TCATTTCCCT ACAGGAACAG TTGCAGCGTT 1441 TTCTGAAGAA GTGCCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1501 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1561 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGACCT CCTCACAATA GAAAGGAAAA 1621 GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t