Transcript: Human NM_024885.4

Homo sapiens TATA-box binding protein associated factor 7 like (TAF7L), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-08-22
Taxon:
Homo sapiens (human)
Gene:
TAF7L (54457)
Length:
2363
CDS:
39..1427

Additional Resources:

NCBI RefSeq record:
NM_024885.4
NBCI Gene record:
TAF7L (54457)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_024885.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000015064 GCCTCTACTGATCCTAATATA pLKO.1 630 CDS 100% 15.000 21.000 N TAF7L n/a
2 TRCN0000015066 CCTGACACTCAAGAATCATTT pLKO.1 1310 CDS 100% 13.200 9.240 N TAF7L n/a
3 TRCN0000015063 GCTCCATAAGATTCAGAATAA pLKO.1 1250 CDS 100% 13.200 9.240 N TAF7L n/a
4 TRCN0000015065 GTCAAGATGAAGGATAAACTA pLKO.1 405 CDS 100% 5.625 3.938 N TAF7L n/a
5 TRCN0000015067 GCAGTTGTTGAAGTAGAAGAT pLKO.1 456 CDS 100% 4.950 3.465 N TAF7L n/a
6 TRCN0000425700 ATCTCATCATGAAAGTGGAAA pLKO_005 1288 CDS 100% 4.950 2.475 Y TAF7L n/a
7 TRCN0000428082 GAGGATGAGGATGAGGATGAA pLKO_005 1071 CDS 100% 4.950 2.475 Y TAF7L n/a
8 TRCN0000153022 GATGAGGATGAGGATGAAGAT pLKO.1 1074 CDS 100% 4.950 2.475 Y OS9 n/a
9 TRCN0000420925 GATGATGAGGATGAGGATGAT pLKO_005 1038 CDS 100% 4.950 2.475 Y TAF7L n/a
10 TRCN0000426897 TGAGGATGAAGATGAAGACAA pLKO_005 1082 CDS 100% 4.950 2.475 Y TAF7L n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_024885.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08377 pDONR223 100% 99.9% 99.7% None 101T>C n/a
2 ccsbBroad304_08377 pLX_304 0% 99.9% 99.7% V5 101T>C n/a
3 TRCN0000477699 CCTCCTCACAATAGAAAGGAAAAG pLX_317 28.8% 99.9% 99.7% V5 101T>C n/a
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