Construct: ORF TRCN0000477700
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012063.1_s317c1
- Derived from:
- ccsbBroadEn_00196
- DNA Barcode:
- TTATACCGTTAGTGTGGATGTCCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMEM50B (757)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477700
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 757 | TMEM50B | transmembrane protein 50B | NM_006134.7 | 100% | 100% | |
| 2 | human | 757 | TMEM50B | transmembrane protein 50B | XM_011529746.2 | 100% | 100% | |
| 3 | human | 757 | TMEM50B | transmembrane protein 50B | NR_040016.1 | 15.1% | 1_213del;688_3127del | |
| 4 | mouse | 77975 | Tmem50b | transmembrane protein 50B | NM_030018.3 | 87.7% | 98.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 540
- ORF length:
- 474
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc aggcttccta gataattttc gttggccaga atgtgaatgt attgactgga 121 gtgagagaag aaatgctgtg gcatctgttg tcgcaggtat attgtttttt acaggctggt 181 ggataatgat tgatGCAGCT GTGGTGTATC CTAAGCCAGA ACAGTTGAAC CATGCCTTTC 241 ACACATGTGG TGTATTTTCC ACATTGGCTT TCTTCATGAT AAATGCTGTA TCCAATGCTC 301 AGGTGAGAGG TGATAGCTAT GAAAGCGGCT GTTTAGGAAG AACAGGTGCT CGAGTTTGGC 361 TTTTCATTGG TTTCATGTTG ATGTTTGGGT CACTTATTGC TTCCATGTGG ATTCTTTTTG 421 GTGCATATGT TACCCAAAAT ACTGATGTTT ATCCGGGACT AGCTGTGTTT TTTCAAAATG 481 CACTTATATT TTTTAGCACT CTGATCTACA AATTTGGAAG AACCGAAGAG CTATGGACCT 541 GCCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 601 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 661 TTTATATATC TTGTGGAAAG GACGATTATA CCGTTAGTGT GGATGTCCAA CGCGTTAAGT 721 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt