Construct: ORF TRCN0000477704
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017848.1_s317c1
- Derived from:
- ccsbBroadEn_12019
- DNA Barcode:
- AAATCCCATCCGTCAGCCTCCAGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CHIC1 (53344)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477704
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 53344 | CHIC1 | cysteine rich hydrophobic d... | NM_001300884.1 | 100% | 100% | |
| 2 | human | 53344 | CHIC1 | cysteine rich hydrophobic d... | NM_001039840.3 | 91% | 91% | 565_624del |
| 3 | human | 53344 | CHIC1 | cysteine rich hydrophobic d... | XM_017029582.1 | 82.3% | 82.8% | 508_513delGTGTGG;516_516delAins103 |
| 4 | human | 53344 | CHIC1 | cysteine rich hydrophobic d... | XM_017029584.1 | 58% | 57.3% | 352_352delAinsGTGGCCCCAGAAG;357_357delCins244 |
| 5 | human | 53344 | CHIC1 | cysteine rich hydrophobic d... | XR_001755699.1 | 45.3% | 1_240del;590_703del;792_793ins174 | |
| 6 | human | 53344 | CHIC1 | cysteine rich hydrophobic d... | XM_017029583.1 | 42.3% | 40.6% | (many diffs) |
| 7 | mouse | 12212 | Chic1 | cysteine-rich hydrophobic d... | XM_006527760.3 | 82% | 84.2% | (many diffs) |
| 8 | mouse | 12212 | Chic1 | cysteine-rich hydrophobic d... | NM_009767.2 | 80.4% | 84.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 681
- ORF length:
- 612
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gagcatcctg ctgcccaaca tggcggagtt cgacaccatc tcggaactgg 121 aggaggagga ggaagaagaa gcggcaacgt cgtcgtcgtc gccgtcgtcg tcgtcgtcgg 181 tatctgggcc cgacgatgac gaggaggatg aggaggaaga ggaggaagag gaggaggagg 241 aagaagagga ggaggaggaa gaggagGAGG AAGCGCCGCC CCCGCCTCGG GTAGTGAGCG 301 AGGAGCATCT GCGGAGATAT GCTCCCGACC CTGTATTAGT GCGGGGTGCC GGCCACATCA 361 CTGTGTTTGG CTTGAGCAAC AAGTTTGATA CTGAATTTCC CTCCGTTCTA ACAGGGAAGG 421 TGGCCCCAGA AGAATTTAAA ACCAGCATTG GCCGTGTGAA TGCATGTTTG AAAAAGGCTC 481 TCCCGGTCAA TGTGAAATGG CTGCTGTGTG GTTGTCTCTG CTGCTGTTGC ACACTGGGTT 541 GCAGCCTATG GCCTGTTATT TGTCTCAACA AAAGAACCAG AAGATCAATT CAGAAGTTAA 601 TAGAATGGGA AAATAACAGA TTATATCACA AGGTAATACT AATAGAATTC TTACCAAAAT 661 ATCCCATATT CCGACCTGAC TTGCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 721 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 781 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAAAAT CCCATCCGTC 841 AGCCTCCAGA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt