Construct: ORF TRCN0000477723

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002590.1_s317c1
Derived from:
ccsbBroadEn_05689
DNA Barcode:
TTCCTAATGGTCCGGTCATACCCC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ARHGEF35 (445328)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000477723
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 445328 ARHGEF35 Rho guanine nucleotide exch... NM_001003702.2 100% 100%
2 human 445328 ARHGEF35 Rho guanine nucleotide exch... NM_001368318.1 100% 100%
3 human 7984 ARHGEF5 Rho guanine nucleotide exch... XM_017012623.2 40.2% 39.7% (many diffs)
4 human 7984 ARHGEF5 Rho guanine nucleotide exch... NM_005435.4 29.9% 29.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1521
ORF length:
1452
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggaggctgag gaggcccagc atggagcctc tcctcccatc tctgccatag
121aggaattcag cattatccct gaggctccca tgaggagcag ccaggtctct gccttggggc
181ttgaagctca agaagatgag gacccatcct ataagtggag agaggaacac agactctcag
241caactcagca gagtgagtta agggatgtgt gtgactatgc gattgagacg atgccctctt
301ttcccaagga aggttctgca gatgtggagc ccaatcagga aagccttgtg gctgaggcct
361gtgacactcc ggaacactgg gaggcagtac cccagagcct agcaggccga caagcaagga
421ctctagctcc cccagagctc tgggcctgcc ccattcagag tgagcatcta gacatggccc
481cattttccag tgacctggga agcgaagaag aggaggtgga attttggcca ggacttactt
541ctttgacatt gggatctgga caggcagaag aagaagagga aacctcttca gataactctg
601gtcagaccag atattattct ccctgcgaag agcatcctgc agagaccaac cagaatgaag
661gcgctgaaag tgggactatc aggcaggggg aagagctgcc atctgaggag ctgcaggaaa
721gtcaagggct cttgcatccc caggaggtcc aagttctgga ggagcaggga cagcaggaag
781caggatttcg gggggaagga actctgaggg aggatgtttg tgccgatggg ctattagggg
841aggaacagat gatagagcag gttaatgatg aaaagggaga acagaagcaa aaacaggaac
901aggtacaaga tgtgatgctt gggagacaag gagaaagaat ggggctcact ggggagccag
961agggtctgaa tgacggtgag tgggagcagg aggatatgga gaggaaggct cagggtcagg
1021gaggtccaga acagggagaa gagaggaaga gggagctgca ggtgccagaa gagaacaggg
1081cggactctca ggacgaaaag agtcaaacct ttttgggaaa atcagaggaa gtaactggaa
1141agcaagaaga tCATGGTATA AAGGAGAAAG GGGTCCCAGT CAGCGGGCAG GAGGCGAAAG
1201AGCCAGAGAG TTGGGATGGG GGCAGGCTGG GGGCAGTGGG AAGAGCGAGG AGCAGGGAAG
1261AGGAGAATGA GCATCATGGG CCTTCAATGC CCGCTCTGAT AGCCCCTGAG GACTCTCCTC
1321ACTGTGACCT GTTTCCAGGT GCCTCATATC TCGTGACTCA GATTCCCGGG ACTCAGACAG
1381AGTCCAGGGC TGAGGAACTG TCCCCCGCAG CTCTGTCTCC CTTGCTAGAG CCCATCAGAT
1441GCTCTCACCA GCCCATTTCT CTACTGGGCT CCTTTTTGAC TGAGGAGTCA CCTGACAAGG
1501AAAAACTTCT ATCAGTACTT TTGCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC
1561CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG
1621AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATTCC TAATGGTCCG
1681GTCATACCCC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)