Construct: ORF TRCN0000477723
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002590.1_s317c1
- Derived from:
- ccsbBroadEn_05689
- DNA Barcode:
- TTCCTAATGGTCCGGTCATACCCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ARHGEF35 (445328)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477723
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 445328 | ARHGEF35 | Rho guanine nucleotide exch... | NM_001003702.2 | 100% | 100% | |
| 2 | human | 445328 | ARHGEF35 | Rho guanine nucleotide exch... | NM_001368318.1 | 100% | 100% | |
| 3 | human | 7984 | ARHGEF5 | Rho guanine nucleotide exch... | XM_017012623.2 | 40.2% | 39.7% | (many diffs) |
| 4 | human | 7984 | ARHGEF5 | Rho guanine nucleotide exch... | NM_005435.4 | 29.9% | 29.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1521
- ORF length:
- 1452
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggaggctgag gaggcccagc atggagcctc tcctcccatc tctgccatag 121 aggaattcag cattatccct gaggctccca tgaggagcag ccaggtctct gccttggggc 181 ttgaagctca agaagatgag gacccatcct ataagtggag agaggaacac agactctcag 241 caactcagca gagtgagtta agggatgtgt gtgactatgc gattgagacg atgccctctt 301 ttcccaagga aggttctgca gatgtggagc ccaatcagga aagccttgtg gctgaggcct 361 gtgacactcc ggaacactgg gaggcagtac cccagagcct agcaggccga caagcaagga 421 ctctagctcc cccagagctc tgggcctgcc ccattcagag tgagcatcta gacatggccc 481 cattttccag tgacctggga agcgaagaag aggaggtgga attttggcca ggacttactt 541 ctttgacatt gggatctgga caggcagaag aagaagagga aacctcttca gataactctg 601 gtcagaccag atattattct ccctgcgaag agcatcctgc agagaccaac cagaatgaag 661 gcgctgaaag tgggactatc aggcaggggg aagagctgcc atctgaggag ctgcaggaaa 721 gtcaagggct cttgcatccc caggaggtcc aagttctgga ggagcaggga cagcaggaag 781 caggatttcg gggggaagga actctgaggg aggatgtttg tgccgatggg ctattagggg 841 aggaacagat gatagagcag gttaatgatg aaaagggaga acagaagcaa aaacaggaac 901 aggtacaaga tgtgatgctt gggagacaag gagaaagaat ggggctcact ggggagccag 961 agggtctgaa tgacggtgag tgggagcagg aggatatgga gaggaaggct cagggtcagg 1021 gaggtccaga acagggagaa gagaggaaga gggagctgca ggtgccagaa gagaacaggg 1081 cggactctca ggacgaaaag agtcaaacct ttttgggaaa atcagaggaa gtaactggaa 1141 agcaagaaga tCATGGTATA AAGGAGAAAG GGGTCCCAGT CAGCGGGCAG GAGGCGAAAG 1201 AGCCAGAGAG TTGGGATGGG GGCAGGCTGG GGGCAGTGGG AAGAGCGAGG AGCAGGGAAG 1261 AGGAGAATGA GCATCATGGG CCTTCAATGC CCGCTCTGAT AGCCCCTGAG GACTCTCCTC 1321 ACTGTGACCT GTTTCCAGGT GCCTCATATC TCGTGACTCA GATTCCCGGG ACTCAGACAG 1381 AGTCCAGGGC TGAGGAACTG TCCCCCGCAG CTCTGTCTCC CTTGCTAGAG CCCATCAGAT 1441 GCTCTCACCA GCCCATTTCT CTACTGGGCT CCTTTTTGAC TGAGGAGTCA CCTGACAAGG 1501 AAAAACTTCT ATCAGTACTT TTGCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1561 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1621 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATTCC TAATGGTCCG 1681 GTCATACCCC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt