Construct: ORF TRCN0000477725
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016339.1_s317c1
- Derived from:
- ccsbBroadEn_06114
- DNA Barcode:
- GCCCGCAAACCTTTGAGACGGGCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TRDMT1 (1787)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477725
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1787 | TRDMT1 | tRNA aspartic acid methyltr... | NM_004412.7 | 99.6% | 99.7% | (many diffs) |
| 2 | human | 1787 | TRDMT1 | tRNA aspartic acid methyltr... | NM_001351219.2 | 97.8% | 97.9% | (many diffs) |
| 3 | human | 1787 | TRDMT1 | tRNA aspartic acid methyltr... | NM_001351220.1 | 94.8% | 92.3% | (many diffs) |
| 4 | human | 1787 | TRDMT1 | tRNA aspartic acid methyltr... | XM_011519343.2 | 94% | 94.1% | (many diffs) |
| 5 | human | 1787 | TRDMT1 | tRNA aspartic acid methyltr... | XM_024447856.1 | 93.6% | 93.8% | (many diffs) |
| 6 | human | 1787 | TRDMT1 | tRNA aspartic acid methyltr... | XM_024447855.1 | 93.1% | 90.5% | (many diffs) |
| 7 | human | 1787 | TRDMT1 | tRNA aspartic acid methyltr... | XM_005252374.5 | 91.3% | 91.5% | (many diffs) |
| 8 | human | 1787 | TRDMT1 | tRNA aspartic acid methyltr... | XM_005252375.4 | 87.9% | 88.2% | (many diffs) |
| 9 | human | 1787 | TRDMT1 | tRNA aspartic acid methyltr... | NM_001351221.2 | 86.1% | 86.4% | (many diffs) |
| 10 | human | 1787 | TRDMT1 | tRNA aspartic acid methyltr... | XM_011519344.2 | 84.6% | 79% | (many diffs) |
| 11 | human | 1787 | TRDMT1 | tRNA aspartic acid methyltr... | XM_011519345.3 | 84.6% | 79% | (many diffs) |
| 12 | human | 1787 | TRDMT1 | tRNA aspartic acid methyltr... | XM_017015801.1 | 83.2% | 80.8% | (many diffs) |
| 13 | human | 1787 | TRDMT1 | tRNA aspartic acid methyltr... | NM_001321007.2 | 81.6% | 81.5% | 0_1ins213;88C>T;336G>T |
| 14 | human | 1787 | TRDMT1 | tRNA aspartic acid methyltr... | XM_017015802.2 | 81.6% | 81.5% | 0_1ins213;88C>T;336G>T |
| 15 | human | 1787 | TRDMT1 | tRNA aspartic acid methyltr... | XM_017015803.2 | 81.6% | 81.5% | 0_1ins213;88C>T;336G>T |
| 16 | human | 1787 | TRDMT1 | tRNA aspartic acid methyltr... | XM_017015804.2 | 81.6% | 81.5% | 0_1ins213;88C>T;336G>T |
| 17 | human | 1787 | TRDMT1 | tRNA aspartic acid methyltr... | XM_017015806.1 | 79.8% | 79.7% | (many diffs) |
| 18 | human | 1787 | TRDMT1 | tRNA aspartic acid methyltr... | NM_001321006.2 | 79% | 73.4% | (many diffs) |
| 19 | human | 1787 | TRDMT1 | tRNA aspartic acid methyltr... | XM_005252378.3 | 76.9% | 74.1% | (many diffs) |
| 20 | human | 1787 | TRDMT1 | tRNA aspartic acid methyltr... | XM_024447857.1 | 76% | 75.9% | (many diffs) |
| 21 | human | 1787 | TRDMT1 | tRNA aspartic acid methyltr... | XM_024447858.1 | 75.6% | 75.7% | 0_1ins213;34_35ins72;264G>T |
| 22 | human | 1787 | TRDMT1 | tRNA aspartic acid methyltr... | NM_001351222.1 | 75.1% | 72.3% | (many diffs) |
| 23 | human | 1787 | TRDMT1 | tRNA aspartic acid methyltr... | NM_001351223.1 | 75.1% | 72.3% | (many diffs) |
| 24 | human | 1787 | TRDMT1 | tRNA aspartic acid methyltr... | XM_017015809.1 | 72.9% | 68% | (many diffs) |
| 25 | human | 1787 | TRDMT1 | tRNA aspartic acid methyltr... | XM_017015810.1 | 71.2% | 68.5% | (many diffs) |
| 26 | human | 1787 | TRDMT1 | tRNA aspartic acid methyltr... | XM_017015812.1 | 69.9% | 70% | 0_1ins213;33_34ins138;198G>T |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1242
- ORF length:
- 1173
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggagcccctg cgggtgctgg agctatacag cggcgtgggc ggcatgcacc 121 acgcgctgag agaaagctgt atacctgcac aagtggtggc tgccattgat gtcaacactg 181 tcgctaatga agtatacaag tataattttc ctcacacaca gttacttgcc aagacaattg 241 aaggcattac actcgaagag tttgacagat tatctttcga tatgatttta atgagccctc 301 cctgccagcc attcacaagg attggccggc agggtgatat gactgattca aggacgaata 361 gcttcttata tattctagat attctcccaa gattacaaaa attaccaaag tatattcttt 421 tggaaaatgt taaaggtttt gaagtatctt ctacaagaga cctcttgata caaacaatag 481 aaaattgtgg ctttcagtac caagagtttc tattatctcc aacctctctt ggcattccaa 541 attcaaggct acgatatttt cttattgcaa agcttcagtc agagccatta ccctttcaag 601 cccctggtca ggtacttatg gagttcccca aaattgaatc tgtacatcca caaaaatatg 661 caatggatgt agaaaataaa attcaagaaa agaacgttga accaaatatt agctttgatg 721 gcagcataca gtgttctgga aaagatgcca ttctttttaa gcttgaaact gcagaagaaa 781 ttcacaggaa aaatcaaCAA GATAGTGATC TCTCTGTGAA AATGCTAAAA GATTTTCTTG 841 AAGATGACAC TGACGTGAAC CAGTATCTTT TACCACCAAA GTCATTGCTG CGATATGCTC 901 TTCTGTTAGA CATTGTTCAG CCCACTTGTA GAAGGTCCGT GTGCTTTACC AAAGGATATG 961 GAAGCTACAT AGAAGGGACA GGGTCTGTGT TACAGACTGC AGAGGATGTG CAGGTTGAGA 1021 ATATCTACAA ATCCCTTACC AATTTGTCAC AAGAAGAACA GATAACAAAG CTGTTAATAC 1081 TTAAACTGCG ATATTTCACT CCTAAAGAAA TAGCAAATCT CCTTGGATTT CCTCCAGAGT 1141 TCGGATTTCC TGAGAAGATA ACAGTGAAAC AGCGTTATCG CCTACTTGGA AATAGTCTCA 1201 ACGTGCATGT AGTAGCTAAA CTAATCAAAA TCTTATATGA ATTGCCAACT TTCTTGTACA 1261 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1321 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1381 AGGACGAGCC CGCAAACCTT TGAGACGGGC GACGCGTTAA GTCgacaatc aacctctgga 1441 ttacaaaatt tgtgaaagat t