Construct: ORF TRCN0000477735
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015939.1_s317c1
- Derived from:
- ccsbBroadEn_08894
- DNA Barcode:
- TCGTTATACATTGCGCACCCGGGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PHACTR4 (65979)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477735
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 65979 | PHACTR4 | phosphatase and actin regul... | NM_001048183.3 | 99.9% | 100% | 1614C>T |
| 2 | human | 65979 | PHACTR4 | phosphatase and actin regul... | NM_001350160.2 | 99.9% | 100% | 1614C>T |
| 3 | human | 65979 | PHACTR4 | phosphatase and actin regul... | NM_001350158.2 | 98.8% | 98.7% | 17_40del;1638C>T |
| 4 | human | 65979 | PHACTR4 | phosphatase and actin regul... | NM_001350159.2 | 98.8% | 98.7% | 17_40del;1638C>T |
| 5 | human | 65979 | PHACTR4 | phosphatase and actin regul... | NM_023923.4 | 98.4% | 98% | (many diffs) |
| 6 | human | 65979 | PHACTR4 | phosphatase and actin regul... | NM_001350161.2 | 97.6% | 97.7% | 0_1ins48;1566C>T |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2172
- ORF length:
- 2106
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga agatccattt gaggaagcag accagcccac tacagagcca ggcatggtcc 121 tggacagtgt ggaagcagga gacacaacac ctcctaccaa aaggaagagc aagttctcag 181 gctttggcaa gatcttcaag ccctggaaat ggaggaaaaa aaaaagtagt gataaattta 241 aagagacttc agaagtttta gaacggaaaa tatctatgcg aaagccaaga gaagagctgg 301 ttaaaagagg ggttctgttg gaagaccctg agcaaggtgg tgaggatcca ggaaagccaa 361 gcgatgccat gttaaagaat ggccatacca cccccatagg gaatgccaga tcatctagtc 421 cagtccaagt agaggaagag ccagtaagat tagcaagtct taggaaagct attccagaag 481 aggacctaaa gaaacgacta ggctcaactg gaagccagcc taattctgaa gcagagtctg 541 ttcctgagaa tgtacccaaa ccacctttac ttcctcccaa aagacccttg tcctcttctc 601 atgaagcaag tgaagggcaa gcaaaggatg ccacttcctc tggcggcacg gcaaggttca 661 tcatctccac ctccatcacc acagcacccg ctgccaccac tgctgccaca agccttgcaa 721 agactgttaa tctctctgtc accccttccc cagcacccag gactctgcct gctgctcctg 781 ccagcactaa cactactgct accccaagcc tcactcatat ggtccctgcc aagcagcccc 841 ctatccctcc ccctaaacca gctcacagaa atagcaaccc tgtcattgct gaactgtccc 901 aagcaataaa cagtggtaca ttgttatcaa aaccgtcccc acccttacca cctaagagag 961 gcattccatc aacctcagta cccaccttgg agtctgctgc tgccatcacc acaaaaacac 1021 caagtgatga aagagagaag agcacgtgtt ctatgggctc ggaactacta ccaatgatct 1081 cacctcgctc tccgtccccc ccactgccta ctcatatacc tccagagcct ccacgcaccc 1141 ctccattccc tgctaagact tttcaagttg tgccagaaat tgagtttcca ccatccttag 1201 atctacacca ggagattccc cagcaggaag atcagaaaaa ggaagtcccc aagaggatac 1261 tggaccagaa ctttggggag ccccatatac cctctaggct gcctccactc ccactgcata 1321 ttcgaatcca gcaggccctc accagcccac ttcccatgac tcctattctg gagggttctc 1381 acagagctca ttcgttgctt tttgaaaaca gtgacagctt ttctgaggac agcagtacgc 1441 tgggtcggac caggtctctt cccatcacta ttgaaatgct aaaagttcca gacgatgaag 1501 aagaagagga gcaaacctgt ccatccacat tcagtgaaga aatgacacct acctcagtca 1561 ttcctaaatt accacagtgt ctacgggagg aagaagagaa ggagagcgac tctgattcag 1621 aaggtcccat tcagtaccga gatgaagaag atgaagatga aagctatcag agtgctcttg 1681 ccaacaaagt gaagaggaaa gacacactgg caatgaagtt gaaccacaga cccagtgaac 1741 cagagttgaa cctgaattct tggccttgta aaagcaaGGA GGAGTGGAAT GAAATACGGC 1801 ACCAGATTGG AAACACACTG ATCCGGCGAC TGAGTCAAAG ACCAACACCA GAAGAACTAG 1861 AACAACGCAA TATATTGCAA CCTAAAAATG AAGCTGATCG TCAGGCAGAA AAACGAGAAA 1921 TTAAACGTCG GCTCACTAGA AAGCTCAGTC AAAGGCCAAC TGTCGCTGAA CTCCTTGCCA 1981 GGAAGATTCT GAGGTTTAAT GAATATGTAG AGGTAACAGA TGCTCAAGAT TATGACCGGC 2041 GAGCCGACAA ACCTTGGACC AAACTGACCC CTGCTGACAA GGCTGCCATA AGAAAAGAAT 2101 TAAATGAATT TAAAAGCTCC GAGATGGAGG TTCATGAAGA GAGCAAACAT TTTACACGCT 2161 ACCATCGTCC ATGCCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 2221 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 2281 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGATCG TTATACATTG CGCACCCGGG 2341 CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t