Construct: ORF TRCN0000477769
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005605.1_s317c1
- Derived from:
- ccsbBroadEn_12093
- DNA Barcode:
- TGAAGACTACGTACATCTATATAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ASPN (54829)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477769
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54829 | ASPN | asporin | NM_017680.5 | 98.6% | 98.6% | 150_151insGATGATGATGATGAG;1113C>T |
| 2 | human | 54829 | ASPN | asporin | NM_001193335.2 | 62.5% | 61.7% | (many diffs) |
| 3 | mouse | 66695 | Aspn | asporin | NM_001172481.1 | 87.1% | 88.8% | (many diffs) |
| 4 | mouse | 66695 | Aspn | asporin | NM_025711.3 | 87.1% | 88.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1221
- ORF length:
- 1152
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaaggagtat gtgctcctat tattcctggc tttgtgctct gccaaaccct 121 tctttagccc ttcacacatc gcactgaaga atatgatgct gaaggatatg gaagacacag 181 atgatgatga tgatgatgat gatgatgatg atgatgatga tgatgatgat gaggaggaca 241 actctctttt tccaacaaga gagccaagaa gccatttttt tccatttgat ctgtttccaa 301 tgtgtccatt tggatgtcag tgctattcac gagttgtaca ttgctcagat ttaggtttga 361 cctcagtccc aaccaacatt ccatttgata ctcgaatgct tgatcttcaa aacaataaaa 421 ttaaggaaat caaagaaaat gattttaaag gactcacttc actttatggt ctgatcctga 481 acaacaacaa gctaacgaag attcacccaa aagcctttct aaccacaaag aagttgcgaa 541 ggctgtatct gtcccacaat caactaagtg aaataccact taatcttccc aaatcattag 601 cagaactcag aattcatgaa aataaagtta agaaaataca aaaggacaca ttcaaaggaa 661 tgaatgcttt acacgttttg gaaatgagtg caaaccctct tgataataat gggatagagc 721 caggggcatt tgaaggggtg acggtgttcc atatcagaat tgcagaagca aaactgacct 781 cagttccTAA AGGCTTACCA CCAACTTTAT TGGAGCTTCA CTTAGATTAT AATAAAATTT 841 CAACAGTGGA ACTTGAGGAT TTTAAACGAT ACAAAGAACT ACAAAGGCTG GGCCTAGGAA 901 ACAACAAAAT CACAGATATC GAAAATGGGA GTCTTGCTAA CATACCACGT GTGAGAGAAA 961 TACATTTGGA AAACAATAAA CTAAAAAAAA TCCCTTCAGG ATTACCAGAG TTGAAATACC 1021 TCCAGATAAT CTTCCTTCAT TCTAATTCAA TTGCAAGAGT GGGAGTAAAT GACTTCTGTC 1081 CAACAGTGCC AAAGATGAAG AAATCTTTAT ACAGTGCAAT AAGTTTATTC AACAACCCGG 1141 TGAAATACTG GGAAATGCAA CCTGCAACAT TTCGTTGTGT TTTGAGCAGA ATGAGTGTTC 1201 AGCTTGGGAA CTTTGGAATG TTGCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1261 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1321 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATGAA GACTACGTAC 1381 ATCTATATAA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt