Construct: ORF TRCN0000477771
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008467.1_s317c1
- Derived from:
- ccsbBroadEn_04485
- DNA Barcode:
- GGACACGGGCGTGCCCGATTCACG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CCDC65 (85478)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477771
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 85478 | CCDC65 | coiled-coil domain containi... | NM_033124.5 | 100% | 100% | |
| 2 | human | 85478 | CCDC65 | coiled-coil domain containi... | NM_001286957.2 | 70.4% | 70.4% | 0_1ins429 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1518
- ORF length:
- 1452
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc taagaaagaa aaaatggcca agacgcccct gtccgatgag aagcagctgc 121 ttctgtttca gcagaagttg ctggcagagg aggagatggc caaaaagaag gagaggctcc 181 tcagccagtt cttgaaggac aagctggcca aggaggaaca caacagtgct ctgaacctta 241 ataagattaa cacacagtgg agaactgtcc ttcgggaagt caagaccaga gaacttcata 301 aggacattga gatcctcagc caaacatttg aacgagtggt ggactgtaag gacaatgtca 361 tcaagtcttt agctaaagac ctgtccgaag ccgaggagca gtacgcccat gccctgcgca 421 gccacttgca caatgttgac cagctcttgg ccctgcagag gcaccggctc agtctcctgg 481 aggaaagtta caacatggag ctggaagccc taaccaagga gtttgagaca gaaaggaaga 541 caattattga ccaacatgag aaagagattc actatctgca agatatcttc atggccatgg 601 agcagaacta tatagattct gagtatgaaa gcaagctgga gttccagagc atgtggaatg 661 atctcaaaaa catgaattta gaagagaagc actttctaag actgcacctg gagaacagag 721 tagaagatct gtggagaaag ttccaggatg tactcaagaa ttacactgat gccacagagg 781 atcgaaaggc tgcctttgag accctgcagg tgaaggatga gaagagctcc aaagagattg 841 aagtacagat gaaaaaaata cagaaactac aggatgccat aactatttca aaaggcaaga 901 tcatgataca cagccgtgag agtgaagatg agaaccggta tatccgtaat gacaaggaat 961 tggtccttgt acaactgcga aaacttaagg cccaaagaac tcaggcccgg gcagcatccc 1021 agaagaactt agtcagactc accctggaaa gtaatgccac cctcaaggcc ctgagaaaga 1081 ttgttgataa gggtgaaaag atccttaaac ttgctgaaat atgtaggaaa tttgaaaCCG 1141 AAGAAGAAAA AGTGCTGCCT TTTTATTCAT CAGTATTGAC TCCTAAGGAG CAGGAGGGGA 1201 TTCAGAAGAA TAATCTAGAA GAGCTTACTG AGGAGCTCAC CAAGGTGATG GTGGACTACA 1261 TAGGAATGGA GAATTTCTGG AAAAGGTACA ACAAAGTGAA ACTGGAGCAA CTGAGCCTCC 1321 AACATAGACG AGCCCAGCTG CTAGATATCA ATGGGAAGCT GCGGGAGATG CTGAAGCAGT 1381 ACTTGGATGG CATCTCAGTG AGTGACGAAG TGCTGAGCCA GCTCAACCCA CTCTTTATAG 1441 TCAACTATCA AAGCAACTTA CTCCAGCCCT TGTCCATACG TATAGCCCAT CCAGGTGATA 1501 AACAACATCC AACCACTTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1561 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1621 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAGGACACG GGCGTGCCCG 1681 ATTCACGACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt