Construct: ORF TRCN0000477772
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014343.1_s317c1
- Derived from:
- ccsbBroadEn_04563
- DNA Barcode:
- TGTGGTCCGCAAAAGACCAATAAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CHRDL1 (91851)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477772
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 91851 | CHRDL1 | chordin like 1 | NM_145234.4 | 100% | 100% | |
| 2 | human | 91851 | CHRDL1 | chordin like 1 | NM_001143982.2 | 99.7% | 99.7% | 983_985delAAG |
| 3 | human | 91851 | CHRDL1 | chordin like 1 | XM_005262222.3 | 99.7% | 99.7% | 983_985delAAG |
| 4 | human | 91851 | CHRDL1 | chordin like 1 | NM_001143981.2 | 99.5% | 99.5% | 300_302delAGA;986_988delAAG |
| 5 | human | 91851 | CHRDL1 | chordin like 1 | NM_001367204.1 | 99.5% | 99.5% | 300_302delAGA;986_988delAAG |
| 6 | human | 91851 | CHRDL1 | chordin like 1 | NM_001367207.1 | 99.1% | 98.9% | 970_971insGTAAAAAAGCAA |
| 7 | human | 91851 | CHRDL1 | chordin like 1 | NM_001367205.1 | 98.9% | 98.6% | 300_302delAGA;973_974insGTAAAAAAGCAA |
| 8 | human | 91851 | CHRDL1 | chordin like 1 | NM_001367206.1 | 98.9% | 98.6% | 300_302delAGA;973_974insGTAAAAAAGCAA |
| 9 | human | 91851 | CHRDL1 | chordin like 1 | NM_001367208.1 | 98.6% | 98.6% | 300_302delAGA;971_972insAGGTAAAAAAGCAAA |
| 10 | human | 91851 | CHRDL1 | chordin like 1 | NM_001367209.1 | 86.6% | 81.7% | (many diffs) |
| 11 | human | 91851 | CHRDL1 | chordin like 1 | NM_001143983.3 | 82.4% | 82.4% | 299_300ins237;746_748delAAG |
| 12 | human | 91851 | CHRDL1 | chordin like 1 | NR_159734.1 | 34% | (many diffs) | |
| 13 | mouse | 83453 | Chrdl1 | chordin-like 1 | NM_001114385.1 | 87.8% | 88.8% | (many diffs) |
| 14 | mouse | 83453 | Chrdl1 | chordin-like 1 | XM_006529003.3 | 87.7% | 88.6% | (many diffs) |
| 15 | mouse | 83453 | Chrdl1 | chordin-like 1 | XM_006529002.3 | 87.5% | 88.4% | (many diffs) |
| 16 | mouse | 83453 | Chrdl1 | chordin-like 1 | XM_006529001.3 | 87.3% | 88.2% | (many diffs) |
| 17 | mouse | 83453 | Chrdl1 | chordin-like 1 | XM_017318643.1 | 87.1% | 88.2% | (many diffs) |
| 18 | mouse | 83453 | Chrdl1 | chordin-like 1 | NM_031258.3 | 65.5% | 65.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1434
- ORF length:
- 1368
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag aaaaaagtgg aaaatgggag gcatgaaata catcttttcg ttgttgttct 121 ttcttttgct agaaggaggc aaaacagagc aagtaaaaca ttcagagaca tattgcatgt 181 ttcaagacaa gaagtacaga gtgggtgaga gatggcatcc ttacctggaa ccttatgggt 241 tggtttactg cgtgaactgc atctgctcag agaatgggaa tgtgctttgc agccgagtca 301 gatgtccaaa tgttcattgc ctttctcctg tgcatattcc tcatctgtgc tgccctcgct 361 gcccagactc cttaccccca gtgaacaata aggtgaccag caagtcttgc gagtacaatg 421 ggacaactta ccaacatgga gagctgttcg tagctgaagg gctctttcag aatcggcaac 481 ccaatcaatg cacccagtgc agctgttcgg agggaaacgt gtattgtggt ctcaagactt 541 gccccaaatt aacctgtgcc ttcccagtct ctgttccaga ttcctgctgc cgggtatgca 601 gaggagatgg agaactgtca tgggaacatt ctgatggtga tatcttccgg caacctgcca 661 acagagaagc aagacattct taccaccgct ctcactatga tcctccacca agccgacagg 721 ctggaggtct gtcccgcttt cctggggcca gaagtcaccg gggagctctt atggattccc 781 agcaagcatc aggaaccatt gtgcaaattg tcatcaataa caaacacaag catggacaag 841 tgtgtgtttc caatggaaag acctattctc atggcgagtc ctggcaccca aacctccggg 901 catttggcat tgtggagtgt gtgctatgta cttgtaatgt caccaagcaa gagtgtaaga 961 aaatccactg ccccaatcga tacccctgca agtatcctca aaaaatagac ggaaaatgct 1021 gcaaggtgtg tccaggtaaa aaagcaaaag aacttccagg ccaaagcttt gacaataaag 1081 gctacttctg cggggaagaa acgatgcctg tgtatgagtc tgtattcatg gaggatgggg 1141 agacaaccag aaaaatagca ctgGAGACTG AGAGACCACC TCAGGTAGAG GTCCACGTTT 1201 GGACTATTCG AAAGGGCATT CTCCAGCACT TCCATATTGA GAAGATCTCC AAGAGGATGT 1261 TTGAGGAGCT TCCTCACTTC AAGCTGGTGA CCAGAACAAC CCTGAGCCAG TGGAAGATCT 1321 TCACCGAAGG AGAAGCTCAG ATCAGCCAGA TGTGTTCAAG TCGTGTATGC AGAACAGAGC 1381 TTGAAGATTT AGTCAAGGTT TTGTACCTGG AGAGATCTGA AAAGGGCCAC TGTTACCCAA 1441 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1501 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1561 TATCTTGTGG AAAGGACGAT GTGGTCCGCA AAAGACCAAT AAGACGCGTT AAGTCgacaa 1621 tcaacctctg gattacaaaa tttgtgaaag att