Construct: ORF TRCN0000477777
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001679.1_s317c1
- Derived from:
- ccsbBroadEn_00256
- DNA Barcode:
- GCGAATACTCCCGTGCCGTCTTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TNFSF8 (944)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477777
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 944 | TNFSF8 | TNF superfamily member 8 | NM_001244.3 | 100% | 100% | |
| 2 | human | 944 | TNFSF8 | TNF superfamily member 8 | XM_024447719.1 | 89.7% | 89.7% | 236_237ins72 |
| 3 | human | 944 | TNFSF8 | TNF superfamily member 8 | NM_001252290.1 | 66.9% | 56.4% | (many diffs) |
| 4 | human | 944 | TNFSF8 | TNF superfamily member 8 | XR_001746422.2 | 51.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 771
- ORF length:
- 702
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggacccaggg ctgcagcaag cactcaacgg aatggcccct cctggagaca 121 cagccatgca tgtgccggcg ggctccgtgg ccagccacct ggggaccacg agccgcagct 181 atttctattt gaccacagcc actctggctc tgtgccttgt cttcacggtg gccactatta 241 tggtgttggt cgttcagagg acggactcca ttcccaactc acctgacaac gtccccctca 301 aaggaggaaa ttgctcagaa gacctcttat gtatcctgaa aagggctcca ttcaagaagt 361 catgggccta cctccaagtg gcaaagcatc taaacaaaac caagttgtct tggaacaaag 421 atggcattct ccatGGAGTC AGATATCAGG ATGGGAATCT GGTGATCCAA TTCCCTGGTT 481 TGTACTTCAT CATTTGCCAA CTGCAGTTTC TTGTACAATG CCCAAATAAT TCTGTCGATC 541 TGAAGTTGGA GCTTCTCATC AACAAGCATA TCAAAAAACA GGCCCTGGTG ACAGTGTGTG 601 AGTCTGGAAT GCAAACGAAA CACGTATACC AGAATCTCTC TCAATTCTTG CTGGATTACC 661 TGCAGGTCAA CACCACCATA TCAGTCAATG TGGATACATT CCAGTACATA GATACAAGCA 721 CCTTTCCTCT TGAGAATGTG TTGTCCATCT TCTTATACAG TAATTCAGAC TTGCCAACTT 781 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 841 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 901 CTTGTGGAAA GGACGAGCGA ATACTCCCGT GCCGTCTTTA ACGCGTTAAG TCgacaatca 961 acctctggat tacaaaattt gtgaaagatt