Construct: ORF TRCN0000477823
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000577.1_s317c1
- Derived from:
- ccsbBroadEn_10129
- DNA Barcode:
- ACTTCTCGTCAACATCTCTATTAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KLHL31 (401265)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477823
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 401265 | KLHL31 | kelch like family member 31 | NM_001003760.5 | 99.7% | 99.8% | (many diffs) |
| 2 | human | 401265 | KLHL31 | kelch like family member 31 | XM_005249107.4 | 99.7% | 99.8% | (many diffs) |
| 3 | human | 401265 | KLHL31 | kelch like family member 31 | XM_017010858.1 | 99.7% | 99.8% | (many diffs) |
| 4 | mouse | 244923 | Klhl31 | kelch-like 31 | NM_172925.2 | 87% | 91.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1971
- ORF length:
- 1902
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcacccaaa aagaagattg tcaaaaagaa caaaggagat atcaatgaga 121 tgactataat cgtagaagat agccccctaa acaaactgaa tgctttgaat gggctcctag 181 agggaggcaa tggccttagc tgcatttctt ctgaactaac agatgcttct tatggcccca 241 acctcttgga aggtttaagt aaaatgcggc aggagaactt cttatgtgac ttagtcattg 301 gtaccaaaac caaatccttt gatgttcata agtcagtcat ggcttcatgc agtgagtatt 361 tttacaacat cctaaaaaaa gacccgtcaa ttcagagggt ggatctcaat gatatctcac 421 cactaggcct ggccactgtc attgcatatg cctacactgg aaagctcact ctctccttgt 481 atacaatagg aagcattatt tctgctgctg tttatcttca gatccatact cttataaaga 541 tgtgcagtga ttttctgata cgggagatga gtgttgagaa ttgcatgtat gttgttaata 601 ttgctgaaac atactcccta aaaaatgcaa aagcagcagc ccagaaattt attcgggata 661 acttccttga atttgcagaa tcggatcagt ttatgaaact tacatttgaa caaattaatg 721 aacttcttat agatgatgac ttacagttgc cttctgagat agtagcattc cagattgcaa 781 tgaaatggtt agaatttgac caaaagagag taaaatacgc tgcagatctt ttgagcaata 841 ttcgctttgg taccatctct gcacaagacc tggtcaatta tgttcaatcc gtaccaagaa 901 tgatgcaaga tgctgattgt cacagacttc tcgtagatgc tatgaactac cacttgcttc 961 catatcatca aaacacattg caatctaggc gaacaagaat ccgaggtggc tgccgagtcc 1021 tcgtcactgt tgggggacgc ccaggcctta ctgagaagtc ccttagcaga gacatcctgt 1081 atagagaccc tgaaaatgga tggagcaagc ttacggaaat gccagccaaa agttttaatc 1141 agtgtgtggc tgtgatggat ggatttcttt atgtagccgg tggtgaagac cagaatgatg 1201 caagaaatca agccaagcat gcagtcagca atttctgcag atacgatccc cgcttcaaca 1261 cctggataca cctggccagc atgaaccaga agcgcacgca cttcagcctg agcgtgttca 1321 acgggctcgt gtacgccgcg ggcggccgca acgcagaagg aagcctggcc tcgctggagt 1381 gctacgtgcc ctccaccaat cagtggcagc cgaagacgcc cctggaggtg gcgcgctgct 1441 gccacgctag cgcggtcgcc gacggccgcg tgctggtgac cggaggctac atcgccaacg 1501 cctactcgcg ctctgtgtgc gccTACGACC CGGCCAGCGA CTCGTGGCAG GAGCTGCCGA 1561 ACCTCAGCAC ACCCCGGGGC TGGCACTGCG CGGTCACGCT GAGCGACAGA GTGTACGTGA 1621 TGGGCGGCAG CCAGCTGGGG CCGCGCGGGG AGCGCGTGGA CGTGCTCACC GTGGAGTGCT 1681 ACAGCCCCGC GACCGGCCAG TGGAGCTACG CGGCGCCGCT GCAGGTGGGA GTGAGCACTG 1741 CGGGCGTCTC GGCGCTGCAT GGCCGCGCCT ACCTGGTGGG GGGCTGGAAC GAGGGCGAGA 1801 AGAAGTACAA GAAGTGCATC CAGTGCTTCA GCCCCGAGCT CAACGAGTGG ACGGAGGACG 1861 ACGAGCTACC CGAGGCCACT GTCGGCGTGT CCTGCTGCAC CCTCTCGATG CCCAACAACG 1921 TGACTCGGGA ATCCCGGGCC AGTTCGGTAT CTTCTGTGCC AGTCAGTATC TTGCCAACTT 1981 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 2041 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 2101 CTTGTGGAAA GGACGAACTT CTCGTCAACA TCTCTATTAC ACGCGTTAAG TCgacaatca 2161 acctctggat tacaaaattt gtgaaagatt