Construct: ORF TRCN0000477825
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014605.1_s317c1
- Derived from:
- ccsbBroadEn_03590
- DNA Barcode:
- TCTCACAATACACAGCTGCCCCCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- YOD1 (55432)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477825
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55432 | YOD1 | YOD1 deubiquitinase | NM_018566.3 | 100% | 100% | |
| 2 | human | 55432 | YOD1 | YOD1 deubiquitinase | NM_001276320.1 | 83.2% | 83.6% | (many diffs) |
| 3 | mouse | 226418 | Yod1 | YOD1 deubiquitinase | NM_178691.4 | 86.8% | 87.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1113
- ORF length:
- 1044
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtttggcccc gctaaaggtc gccattttgg agtccacccg gcgcctggtt 121 tccccggcgg cgtctcccaa caggctgccg ggaccaaagc tggccccgcg ggtgcctggc 181 ctgtgggcag ccggaccgac acgatgtggc ggctccgctg caaggccaag gacggcaccc 241 atgttttgca ggggctgtcc agccggaccc gggtgcggga actccagggc caaattgccg 301 ccatcaccgg gatcgccccc ggcggtcagc gaatcctcgt cggataccct cccgagtgcc 361 tggatctcag caatggggat accattctgg aagacttgcc catccaatct ggtgacatgc 421 tgatcattga agaagaccaa accaggccca gaagttcacc tgcatttact aaacgtggtg 481 cttctagtta cgtcagggaa actttgcctg tgcttaccag aaccgtggtc ccagcagaca 541 actcttgcct ctttactagt gtgtactatg tcgtcgaagg aggagtcttg aatccagctt 601 gtgcccctga gatgagacgc ctcatagcac aaattgtagc aagcgatcca gacttctata 661 gtgaggcaat actgggaaaa acaaaTCAAG AGTACTGTGA CTGGATCAAA AGGGATGACA 721 CTTGGGGAGG AGCAATAGAG ATATCGATTT TGTCCAAGTT TTACCAATGT GAAATATGTG 781 TAGTGGATAC ACAGACAGTA AGAATTGATC GTTTTGGGGA AGATGCAGGA TATACCAAAA 841 GGGTTCTGCT TATTTATGAT GGCATCCACT ATGATCCACT TCAGCGTAAC TTCCCTGATC 901 CAGATACACC TCCTCTGACC ATTTTCTCCT CTAATGATGA TATTGTTCTT GTACAAGCAC 961 TGGAATTAGC AGATGAAGCT AGAAGAAGGA GACAGTTTAC TGATGTCAAC CGCTTCACCC 1021 TGAGATGCAT GGTATGTCAG AAAGGATTAA CTGGACAAGC AGAAGCAAGG GAACATGCCA 1081 AGGAGACAGG CCATACCAAC TTTGGAGAAG TGTTGCCAAC TTTCTTGTAC AAAGTGGTTG 1141 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1201 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATC 1261 TCACAATACA CAGCTGCCCC CGACGCGTTA AGTCgacaat caacctctgg attacaaaat 1321 ttgtgaaaga tt