Construct: ORF TRCN0000477828
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010280.1_s317c1
- Derived from:
- ccsbBroadEn_09401
- DNA Barcode:
- GATGGCCCCGTTTTGCACTAACTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMEM200A (114801)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477828
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 114801 | TMEM200A | transmembrane protein 200A | NM_001258276.1 | 99.8% | 100% | 231T>C;390A>G |
| 2 | human | 114801 | TMEM200A | transmembrane protein 200A | NM_001258277.1 | 99.8% | 100% | 231T>C;390A>G |
| 3 | human | 114801 | TMEM200A | transmembrane protein 200A | NM_001258278.1 | 99.8% | 100% | 231T>C;390A>G |
| 4 | human | 114801 | TMEM200A | transmembrane protein 200A | NM_052913.2 | 99.8% | 100% | 231T>C;390A>G |
| 5 | human | 114801 | TMEM200A | transmembrane protein 200A | XM_005266816.3 | 99.8% | 100% | 231T>C;390A>G |
| 6 | human | 114801 | TMEM200A | transmembrane protein 200A | XM_011535419.2 | 99.8% | 100% | 231T>C;390A>G |
| 7 | human | 114801 | TMEM200A | transmembrane protein 200A | XM_017010230.2 | 99.8% | 100% | 231T>C;390A>G |
| 8 | mouse | 77220 | Tmem200a | transmembrane protein 200A | NM_029881.3 | 82.2% | 90.6% | (many diffs) |
| 9 | mouse | 77220 | Tmem200a | transmembrane protein 200A | XM_006512919.3 | 82.2% | 90.6% | (many diffs) |
| 10 | mouse | 77220 | Tmem200a | transmembrane protein 200A | XM_006512920.2 | 82.2% | 90.6% | (many diffs) |
| 11 | mouse | 77220 | Tmem200a | transmembrane protein 200A | XM_006512921.3 | 82.2% | 90.6% | (many diffs) |
| 12 | mouse | 77220 | Tmem200a | transmembrane protein 200A | XM_017314147.1 | 81.1% | 89.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1542
- ORF length:
- 1473
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gatagcaact ggtggagtga taactggcct ggccgccttg aaaaggcaag 121 actctgccag atcacagcag catgtcaacc tcagcccgtc tcctgctacc caagagaaga 181 agcccatcag gcgccggccc cgggcagatg ttgtggttgt tcgtggcaaa atccggcttt 241 attccccatc tggttttttt cttattttag gagtgctcat ctccattata ggaattgcca 301 tggccgttct tggatattgg ccccaaaaag aacattttat tgatgctgaa acaacactgt 361 caacaaatga aactcaggtc attcggaatg aaggcggtgt ggtggttcgc ttctttgagc 421 agcatttgca ttctgataag atgaaaatgc ttggcccgtt caccatgggg attggcattt 481 tcattttcat ttgtgctaat gccattcttc atgaaaaccg tgacaaagag accaaaatca 541 tacacatgag ggatatctat tccacagtca ttgacattca cacgctaaga atcaaggagc 601 aaaggcaaat gaacggcatg tacactggtt tgatgggaga aacagaagta aaacagaatg 661 ggagctcctg tgcctcgaga ttggcagcaa atacgatcgc ctctttctcg ggttttcgga 721 gcagttttcg aatggacagc tccgtggagg aggatgaact tatgttaaat gaaggtaaga 781 gttctgggca tcttatgccc cctttgctct ctgacagctc tgtgtctgtc tttggcctct 841 atccacctcc ttccaagaca actgatgata agaccagcgg ctctaagaaa tgtgaaacca 901 agtcaattgt gtcatcgtcc atcagtgctt ttacattgcc tgtgatcaaa cttaataact 961 gtgttattga tgagcccagt atagataaca tcactgaaga tgctgacaac ctcaaaagta 1021 ggtcaaggaa tttgtcaatg gattcccttg tggttccttt gcccaacacc agtgaatcct 1081 tccagcccgt cagcacagtg ctaccaagga ataattccat tggggagtcg ttgtcgagtc 1141 agtacaagtc atctatggct cTCGGACCTG GGGCTGGACA GCTCTTGTCT CCTGGGGCTG 1201 CCAGAAGACA GTTTGGGTCC AATACATCCT TGCATTTGCT CTCGTCACAC TCAAAGTCCT 1261 TGGACTTAGA CCGGGGTCCC TCCACTCTAA CTGTTCAGGC AGAACAACGG AAACATCCAA 1321 GTTGGCCTAG GTTGGATCGG AACAACAGCA AGGGATATAT GAAACTAGAG AACAAAGAAG 1381 ACCCGATGGA TAGGTTGCTT GTGCCCCAAG TTGCCATCAA AAAGGACTTT ACCAATAAGG 1441 AGAAGCTTCT TATGATTTCA AGATCTCACA ATAATTTGAG TTTTGAACAT GATGAGTTTT 1501 TGAGTAACAA CCTAAAGAGG GGAACTTCTG AAACAAGGTT TTTGCCAACT TTCTTGTACA 1561 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1621 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1681 AGGACGAGAT GGCCCCGTTT TGCACTAACT GACGCGTTAA GTCgacaatc aacctctgga 1741 ttacaaaatt tgtgaaagat t