Construct: ORF TRCN0000477828

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010280.1_s317c1
Derived from:
ccsbBroadEn_09401
DNA Barcode:
GATGGCCCCGTTTTGCACTAACTG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TMEM200A (114801)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000477828
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 114801 TMEM200A transmembrane protein 200A NM_001258276.1 99.8% 100% 231T>C;390A>G
2 human 114801 TMEM200A transmembrane protein 200A NM_001258277.1 99.8% 100% 231T>C;390A>G
3 human 114801 TMEM200A transmembrane protein 200A NM_001258278.1 99.8% 100% 231T>C;390A>G
4 human 114801 TMEM200A transmembrane protein 200A NM_052913.2 99.8% 100% 231T>C;390A>G
5 human 114801 TMEM200A transmembrane protein 200A XM_005266816.3 99.8% 100% 231T>C;390A>G
6 human 114801 TMEM200A transmembrane protein 200A XM_011535419.2 99.8% 100% 231T>C;390A>G
7 human 114801 TMEM200A transmembrane protein 200A XM_017010230.2 99.8% 100% 231T>C;390A>G
8 mouse 77220 Tmem200a transmembrane protein 200A NM_029881.3 82.2% 90.6% (many diffs)
9 mouse 77220 Tmem200a transmembrane protein 200A XM_006512919.3 82.2% 90.6% (many diffs)
10 mouse 77220 Tmem200a transmembrane protein 200A XM_006512920.2 82.2% 90.6% (many diffs)
11 mouse 77220 Tmem200a transmembrane protein 200A XM_006512921.3 82.2% 90.6% (many diffs)
12 mouse 77220 Tmem200a transmembrane protein 200A XM_017314147.1 81.1% 89.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1542
ORF length:
1473
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gatagcaact ggtggagtga taactggcct ggccgccttg aaaaggcaag
121actctgccag atcacagcag catgtcaacc tcagcccgtc tcctgctacc caagagaaga
181agcccatcag gcgccggccc cgggcagatg ttgtggttgt tcgtggcaaa atccggcttt
241attccccatc tggttttttt cttattttag gagtgctcat ctccattata ggaattgcca
301tggccgttct tggatattgg ccccaaaaag aacattttat tgatgctgaa acaacactgt
361caacaaatga aactcaggtc attcggaatg aaggcggtgt ggtggttcgc ttctttgagc
421agcatttgca ttctgataag atgaaaatgc ttggcccgtt caccatgggg attggcattt
481tcattttcat ttgtgctaat gccattcttc atgaaaaccg tgacaaagag accaaaatca
541tacacatgag ggatatctat tccacagtca ttgacattca cacgctaaga atcaaggagc
601aaaggcaaat gaacggcatg tacactggtt tgatgggaga aacagaagta aaacagaatg
661ggagctcctg tgcctcgaga ttggcagcaa atacgatcgc ctctttctcg ggttttcgga
721gcagttttcg aatggacagc tccgtggagg aggatgaact tatgttaaat gaaggtaaga
781gttctgggca tcttatgccc cctttgctct ctgacagctc tgtgtctgtc tttggcctct
841atccacctcc ttccaagaca actgatgata agaccagcgg ctctaagaaa tgtgaaacca
901agtcaattgt gtcatcgtcc atcagtgctt ttacattgcc tgtgatcaaa cttaataact
961gtgttattga tgagcccagt atagataaca tcactgaaga tgctgacaac ctcaaaagta
1021ggtcaaggaa tttgtcaatg gattcccttg tggttccttt gcccaacacc agtgaatcct
1081tccagcccgt cagcacagtg ctaccaagga ataattccat tggggagtcg ttgtcgagtc
1141agtacaagtc atctatggct cTCGGACCTG GGGCTGGACA GCTCTTGTCT CCTGGGGCTG
1201CCAGAAGACA GTTTGGGTCC AATACATCCT TGCATTTGCT CTCGTCACAC TCAAAGTCCT
1261TGGACTTAGA CCGGGGTCCC TCCACTCTAA CTGTTCAGGC AGAACAACGG AAACATCCAA
1321GTTGGCCTAG GTTGGATCGG AACAACAGCA AGGGATATAT GAAACTAGAG AACAAAGAAG
1381ACCCGATGGA TAGGTTGCTT GTGCCCCAAG TTGCCATCAA AAAGGACTTT ACCAATAAGG
1441AGAAGCTTCT TATGATTTCA AGATCTCACA ATAATTTGAG TTTTGAACAT GATGAGTTTT
1501TGAGTAACAA CCTAAAGAGG GGAACTTCTG AAACAAGGTT TTTGCCAACT TTCTTGTACA
1561AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT
1621AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA
1681AGGACGAGAT GGCCCCGTTT TGCACTAACT GACGCGTTAA GTCgacaatc aacctctgga
1741ttacaaaatt tgtgaaagat t

Download FASTA (ORF) (Full)

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