Construct: ORF TRCN0000477831
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010824.1_s317c1
- Derived from:
- ccsbBroadEn_05505
- DNA Barcode:
- ATACCTCGACAGGCTTTCCATGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KLRG2 (346689)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477831
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 346689 | KLRG2 | killer cell lectin like rec... | NM_198508.4 | 100% | 100% | |
| 2 | human | 346689 | KLRG2 | killer cell lectin like rec... | XM_011516141.2 | 83.4% | 81.7% | (many diffs) |
| 3 | human | 346689 | KLRG2 | killer cell lectin like rec... | XM_005250311.3 | 82.3% | 81.9% | 1004_1005insG;1009C>T;1011_1012ins215 |
| 4 | human | 346689 | KLRG2 | killer cell lectin like rec... | XM_011516140.2 | 82% | 83.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1296
- ORF length:
- 1227
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggaggagtct tgggaggctg cgcccggagg ccaagccggg gcagagctcc 121 caatggagcc cgtgggaagc ctggtcccca cgctggagca gccgcaggtg cccgcgaagg 181 tgcgacaacc tgaaggtccc gaaagcagcc caagtccggc cggggccgtg gagaaggcgg 241 cgggcgcagg cctggagccc tcgagcaaga aaaagccgcc ttcgcctcgc cccgggtccc 301 cgcgcgtgcc gccgctcagc ctgggctacg gggtctgccc cgagccgccg tcaccgggcc 361 ctgccttggt caagctgccc cggaatggcg aggcgcccgg ggctgagcct gcgcccagcg 421 cctgggcgcc catggagctg caggtagatg tgcgcgtgaa gcccgtgggc gcggccggtg 481 gcagcagcac gccatcgccc aggccctcca cgcgcttcct caaggtgccg gtgcccgagt 541 cccctgcctt ctcccgccac gcggacccgg cgcaccagct cctgctgcgc gcaccatccc 601 agggcggcac gtggggccgc cgctcgccgc tggctgcagc ccggacggag agcggctgcg 661 acgcagaggg ccgggccagc cccgcggaag gaagcgccgg ctccccgggc tcccccacgt 721 gctgccgctg caaggagctg gggctggaga aggaggatgc ggcgctgttg ccccgcgcgg 781 ggttggacgg cgacgagaag ctgccccggg ccgtaacgct tacggggcta cccatgtacg 841 tgaagtccct gtactgggcc ctggcgttca tggctgtgct cctggcagtc tctggggttg 901 tcattgtggt cctggcctca agagcaggag ccagatgcca gcagtgcccc ccaggctggg 961 tgttgtccga ggagcactgt tactacttct ctgcagaagc gcaggcctgg gaagccagcc 1021 aggctttctg ctcagcctac cacgctaccc tccccctgct aagccacacc caggacttcc 1081 tgggcagata cccagtctcc aggcactcct gggtgggggc ctggcgaggc ccccagggct 1141 ggcactggat cGACGAGGCC CCACTCCCGC CCCAGCTACT CCCTGAGGAC GGCGAGGACA 1201 ATCTGGATAT CAACTGTGGG GCCCTGGAGG AAGGCACGCT GGTGGCTGCA AACTGCAGCA 1261 CTCCAAGACC CTGGGTCTGT GCCAAGGGGA CCCAGTTGCC AACTTTCTTG TACAAAGTGG 1321 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1381 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1441 AATACCTCGA CAGGCTTTCC ATGCAACGCG TTAAGTCgac aatcaacctc tggattacaa 1501 aatttgtgaa agatt