Construct: ORF TRCN0000477833
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007494.1_s317c1
- Derived from:
- ccsbBroadEn_07431
- DNA Barcode:
- CTCAGGATAACCTCTGATATCCAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RPH3AL (9501)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477833
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9501 | RPH3AL | rabphilin 3A like (without ... | NM_001190412.2 | 99.8% | 99.6% | 254G>A |
| 2 | human | 9501 | RPH3AL | rabphilin 3A like (without ... | NM_001190413.2 | 99.8% | 99.6% | 254G>A |
| 3 | human | 9501 | RPH3AL | rabphilin 3A like (without ... | NM_001190411.1 | 90.6% | 90.4% | 254G>A;350_436del |
| 4 | human | 9501 | RPH3AL | rabphilin 3A like (without ... | NM_006987.4 | 90.6% | 90.4% | 254G>A;350_436del |
| 5 | mouse | 380714 | Rph3al | rabphilin 3A-like (without ... | XM_011249100.1 | 51.3% | 51.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 67
- ORF end:
- 925
- ORF length:
- 858
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggccatgg ccgacaccat cttcggcagc gggaatgatc agtgggtttg ccccaatgac 121 cggcagcttg cccttcgagc caagctgcag acgggctggt ccgtgcacac ctaccagacg 181 gagaagcaga ggaggaagca gcacctcagc ccggcggagg tggaggccat cctgcaggtc 241 atccagaggg cagagcggct cgacgtcctg gagcagcaga gaatcgggcg gctggtggag 301 cggctggaga ccatgaggca gaatgtgatg gggaacggcc tgtcccagtg tctgctctgc 361 ggggaggtgc tgggcttcct gggcagctcg tcggtgttct gcaaagactg caggaaggtc 421 tggaagaggt cgggggcctg gttctacaaa gggctcccca agtatatctt gcccctgaag 481 acccctggcc gagctgatga cccccacttc cgacctttgc ccacggaacc ggcagagcga 541 gagcccagaa gctctgagac cagccgcatc tacacgtggg cccgaggaag agtggtttcc 601 agtgacagtg acagtgactc ggatcttagc tcctccagcc tagaggacag actcccatcc 661 actggggtca gggaccggAA AGGCGACAAA CCCTGGAAGG AGTCAGGTGG CAGCGTGGAG 721 GCCCCCAGGA TGGGGTTCAC CCACCCGCCG GGCCACCTCT CTGGGTGCCA GAGCAGCCTG 781 GCCAGTGGTG AGACGGGGAC AGGCTCTGCT GACCCGCCAG GGGGACCCCG CCCCGGGCTG 841 ACCCGAAGGG CCCCGGTAAA AGACACACCT GGACGAGCCC CCGCTGCTGA CGCAGCTCCA 901 GCAGGCCCCT CCAGCTGCCT GGGCTTGCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT 961 AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA 1021 CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CTCAGGATAA 1081 CCTCTGATAT CCAAACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa 1141 gatt