Transcript: Human NM_006987.4

Homo sapiens rabphilin 3A like (without C2 domains) (RPH3AL), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-09-27
Taxon:
Homo sapiens (human)
Gene:
RPH3AL (9501)
Length:
2719
CDS:
309..1256

Additional Resources:

NCBI RefSeq record:
NM_006987.4
NBCI Gene record:
RPH3AL (9501)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_006987.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000298647 GGCTATCTCCGTTGCTATATT pLKO_005 1608 3UTR 100% 15.000 21.000 N RPH3AL n/a
2 TRCN0000059867 AGGGACCGGAAAGGCGACAAA pLKO.1 999 CDS 100% 1.650 2.310 N RPH3AL n/a
3 TRCN0000298245 AGGGACCGGAAAGGCGACAAA pLKO_005 999 CDS 100% 1.650 2.310 N RPH3AL n/a
4 TRCN0000115353 GCTGTGTAAGATCTGCAGTGA pLKO.1 716 CDS 100% 2.640 2.112 N Rph3al n/a
5 TRCN0000059866 GCAGTGAGCAAAGAGAGGTCT pLKO.1 730 CDS 100% 2.640 1.848 N RPH3AL n/a
6 TRCN0000311678 GCAGTGAGCAAAGAGAGGTCT pLKO_005 730 CDS 100% 2.640 1.848 N RPH3AL n/a
7 TRCN0000298645 TAGAGGACAGACTCCCATCCA pLKO_005 970 CDS 100% 2.640 1.848 N RPH3AL n/a
8 TRCN0000059865 CCTGGGCAGCTCGTCGGTGTT pLKO.1 620 CDS 100% 0.000 0.000 N RPH3AL n/a
9 TRCN0000059864 CAGGAAGAAAGTCTGCACCAA pLKO.1 653 CDS 100% 2.640 1.584 N RPH3AL n/a
10 TRCN0000286500 CAGGAAGAAAGTCTGCACCAA pLKO_005 653 CDS 100% 2.640 1.584 N RPH3AL n/a
11 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 2094 3UTR 100% 5.625 2.813 Y KLHL30 n/a
12 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 2094 3UTR 100% 5.625 2.813 Y EID2B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_006987.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02176 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_02176 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000469660 GCCGCCTCGGATATTAGTTAGCCG pLX_317 31.5% 100% 100% V5 n/a
4 ccsbBroadEn_07431 pDONR223 100% 90.6% 90.4% None 254G>A;350_436del n/a
5 ccsbBroad304_07431 pLX_304 0% 90.6% 90.4% V5 254G>A;350_436del n/a
6 TRCN0000477833 CTCAGGATAACCTCTGATATCCAA pLX_317 29.2% 90.6% 90.4% V5 254G>A;350_436del n/a
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