Construct: ORF TRCN0000477875
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013864.1_s317c1
- Derived from:
- ccsbBroadEn_04954
- DNA Barcode:
- ACCATGGGCGGGACACCCTTGCAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- WFDC6 (140870)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477875
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 140870 | WFDC6 | WAP four-disulfide core dom... | NM_080827.2 | 100% | 100% | |
| 2 | human | 100526773 | EPPIN-WFDC6 | EPPIN-WFDC6 readthrough | NM_001198986.2 | 31% | 32.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 327
- ORF length:
- 258
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gggactctca ggacttctgc caatcctggt accattcatc cTTTTGGGGG 121 ACATCCAGGA ACCTGGGCAC GCTGAAGGCA TCCTTGGCAA GCCGTGTCCC AAAATCAAAG 181 TGGAATGCGA AGTGGAAGAA ATAGACCAGT GTACCAAACC CAGAGATTGC CCAGAAAACA 241 TGAAGTGTTG CCCGTTCAGC CGTGGAAAGA AATGTTTAGA CTTCAGAAAG GTCAGCCTTA 301 CTTTATACCA TAAGGAGGAG CTTGAATTGC CAACTTTCTT GTACAAAGTG GTTGATATCG 361 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 421 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAACCATGGG 481 CGGGACACCC TTGCACACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 541 aagatt