Construct: ORF TRCN0000477875

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF013864.1_s317c1
Derived from:
ccsbBroadEn_04954
DNA Barcode:
ACCATGGGCGGGACACCCTTGCAC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
WFDC6 (140870)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000477875
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 140870 WFDC6 WAP four-disulfide core dom... NM_080827.2 100% 100%
2 human 100526773 EPPIN-WFDC6 EPPIN-WFDC6 readthrough NM_001198986.2 31% 32.7% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
327
ORF length:
258
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gggactctca ggacttctgc caatcctggt accattcatc cTTTTGGGGG
121ACATCCAGGA ACCTGGGCAC GCTGAAGGCA TCCTTGGCAA GCCGTGTCCC AAAATCAAAG
181TGGAATGCGA AGTGGAAGAA ATAGACCAGT GTACCAAACC CAGAGATTGC CCAGAAAACA
241TGAAGTGTTG CCCGTTCAGC CGTGGAAAGA AATGTTTAGA CTTCAGAAAG GTCAGCCTTA
301CTTTATACCA TAAGGAGGAG CTTGAATTGC CAACTTTCTT GTACAAAGTG GTTGATATCG
361GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT
421AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAACCATGGG
481CGGGACACCC TTGCACACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga
541aagatt

Download FASTA (ORF) (Full)