Construct: ORF TRCN0000477892
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014047.1_s317c1
- Derived from:
- ccsbBroadEn_09661
- DNA Barcode:
- AAATTTCACGTCACTCGCACAGCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FAM78B (149297)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477892
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 149297 | FAM78B | family with sequence simila... | NM_001017961.5 | 99.8% | 100% | 366G>C |
| 2 | human | 149297 | FAM78B | family with sequence simila... | XM_017000417.2 | 99.8% | 100% | 366G>C |
| 3 | human | 149297 | FAM78B | family with sequence simila... | NM_001320302.1 | 38% | 33.9% | (many diffs) |
| 4 | human | 149297 | FAM78B | family with sequence simila... | NR_163271.1 | 12.1% | 1_507del;873G>C;1291_6445del | |
| 5 | human | 149297 | FAM78B | family with sequence simila... | NR_135199.1 | 12.1% | 1_507del;873G>C;1291_6456del | |
| 6 | mouse | 226610 | Fam78b | family with sequence simila... | NM_001160261.1 | 95.9% | 100% | (many diffs) |
| 7 | mouse | 226610 | Fam78b | family with sequence simila... | NM_001160262.1 | 95.9% | 100% | (many diffs) |
| 8 | mouse | 226610 | Fam78b | family with sequence simila... | NM_175461.4 | 95.9% | 100% | (many diffs) |
| 9 | mouse | 226610 | Fam78b | family with sequence simila... | XM_006496789.1 | 95.9% | 100% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 852
- ORF length:
- 783
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gggctgtatc caaagcatca cctgcaaggc gcggatccgg cgcgagaaca 121 tcgtggtgta cgatgtgtgc gccaccatcg accagtgccc cacgcgcatc gaggagacct 181 cgcccatcgt cctgcgctac aagaccccct acttcaaagc ctccgcccgc gtggtcatgc 241 cccccatccc ccgccacgag acctgggtgg tgggctggat tcaggcgtgc aatcagatgg 301 agttcttcaa cacctacagc gacctgggca tgtcaagctg ggaactgcct gacttgaggg 361 aagggagagt aaaagccatc agtgactcag atggggtgag ctacccttgg tacgggaaca 421 ccacagaaac tgtcaccctg gttggcccca ccaacaagat ctccaggttc TCCGTCAGCA 481 TGAATGACAA CTTCTACCCC AGTGTGACAT GGGCAGTGCC TGTGAGTGAC AGCAATGTGC 541 CACTGCTCAC AAGAATCAAG AGAGACCAAA GTTTCACGAC CTGGCTGGTG GCCATGAACA 601 CCACCACAAA GGAGAAGATC ATTCTGCAGA CCATCAAGTG GAGGATGAGG GTGGACATTG 661 AAGTGGACCC TCTTCAGCTC TTGGGGCAGC GGGCCCGGCT GGTGGGCAGG ACTCAGCAGG 721 AGCAGCCCCG GATCCTGAGC CGGATGGAAC CCATCCCCCC TAATGCACTA GTGAAACCCA 781 ATGCCAATGA TGCCCAGGTC CTCATGTGGA GGCCCAAGCG GGGGCCACCT CTGGTTGTGA 841 TCCCTCCTAA GTTGCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 901 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 961 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAAAA TTTCACGTCA CTCGCACAGC 1021 TACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t