Construct: ORF TRCN0000477900
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001996.1_s317c1
- Derived from:
- ccsbBroadEn_10481
- DNA Barcode:
- CCCAATGTTACCCCCAAGCTGTAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- VXN (254778)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477900
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 254778 | VXN | vexin | NM_152765.4 | 99.5% | 99.5% | 1_3delATG |
| 2 | mouse | 76982 | 3110035E14Rik | RIKEN cDNA 3110035E14 gene | NM_178399.4 | 83.6% | 82.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 684
- ORF length:
- 618
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca tcagatttac agctgcagtg acgagaacat agaagttttc accaccgtga 121 ttccttccaa ggtatccagt ccagccagaa gaagagccaa aagctctcag cacctcttga 181 ccaagaatgt ggtgatcgag tcggacctgt acacgcacca gcccctggag ctgctgcccc 241 accgcggaga ccgcagggac cctggcgacc gccgcaggtt tgggcggctc cagaccgcgc 301 ggccgcccac agcccacccg gccaaagccT CTGCCAGACC CGTGGGGATT TCTGAACCCA 361 AAACATCAAA TCTGTGTGGG AATCGAGCAT ATGGAAAATC TCTGATACCG CCAGTGCCCC 421 GGATCTCAGT GAAAACTTCA GCCTCTGCCT CATTGGAGGC GACAGCCATG GGCACAGAGA 481 AGGGAGCTGT TCTGATGAGA GGATCCAGAC ATCTCAAGAA GATGACTGAA GAGTATCCAG 541 CCCTTCCCCA AGGAGCAGAA GCCTCTCTAC CACTGACAGG CAGTGCTTCC TGCGGCGTCC 601 CCGGCATCCT CCGGAAAATG TGGACAAGGC ACAAGAAGAA GTCTGAATAT GTGGGAGCCA 661 CCAACAGCGC CTTTGAGGCC GACTATCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 721 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 781 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAC CCAATGTTAC 841 CCCCAAGCTG TAGACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 901 att