Construct: ORF TRCN0000477915
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003186.1_s317c1
- Derived from:
- ccsbBroadEn_05611
- DNA Barcode:
- AATTGTGAGTTCGGCTCTCGCGTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- OR4D10 (390197)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477915
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 390197 | OR4D10 | olfactory receptor family 4... | NM_001004705.1 | 100% | 100% | |
| 2 | mouse | 258155 | Olfr1425 | olfactory receptor 1425 | NM_001011853.1 | 85% | 85.6% | (many diffs) |
| 3 | mouse | 258805 | Olfr1426 | olfactory receptor 1426 | NM_146809.2 | 83.8% | 84.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1002
- ORF length:
- 933
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggagatggaa aactgcacca gggtaaaaga atttattttc cttggcctga 121 cccagaatcg ggaagtgagc ttagtcttat ttcttttcct actcttggtg tatgtgacaa 181 ctttgctggg aaacctcctc atcatggtca ctgttacctg tgaatctcgc cttcacacgc 241 ccatgtattt tttgctccat aatttatcta ttgccgatat ctgcttctct tccatcacag 301 tgcccaaggt tctggtggac cttctgtctg aaagaaagac catctccttc aatcattgct 361 tcactcagat gtttctattc caccttattg gaggggtgga tgtattttct ctttcggtga 421 tggcattgga tcgatatgtg gccatctcca agcccctgca ctatgcgact atcatgagta 481 gagaccattg cattgggctc acagtggctg cctggttggg gggctttgtc cactccatcg 541 tgcagatttc cctgttgctc ccactccctt tctgcggacc caatgttctt gacactttct 601 actgtgatgt ccaccgggtc ctcaaactgg cccatacaga cattttcata cttgaactac 661 TAATGATTTC CAACAATGGA CTGCTCACCA CACTGTGGTT TTTCCTGCTC CTGGTGTCCT 721 ACATAGTCAT ATTATCATTA CCCAAGTCTC AGGCAGGAGA GGGCAGGAGG AAAGCCATCT 781 CCACCTGCAC CTCCCACATC ACTGTGGTGA CCCTGCATTT CGTGCCCTGC ATCTATGTCT 841 ATGCCCGGCC CTTCACTGCC CTCCCCATGG ATAAGGCCAT CTCTGTCACC TTCACTGTCA 901 TCTCCCCTCT GCTCAACCCC TTGATCTACA CTCTGAGGAA CCATGAGATG AAGTCAGCCA 961 TGAGGAGACT GAAGAGAAGA CTTGTGCCTT CTGATAGAAA ATTGCCAACT TTCTTGTACA 1021 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1081 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1141 AGGACGAAAT TGTGAGTTCG GCTCTCGCGT AACGCGTTAA GTCgacaatc aacctctgga 1201 ttacaaaatt tgtgaaagat t