Construct: ORF TRCN0000477925

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010277.1_s317c1
Derived from:
ccsbBroadEn_13006
DNA Barcode:
ACATATATAATGGTCGTCTGCCTC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ARHGAP12 (94134)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000477925
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 94134 ARHGAP12 Rho GTPase activating prote... NM_001270695.1 99.9% 99.8% 2518G>C
2 human 94134 ARHGAP12 Rho GTPase activating prote... NM_018287.7 99.3% 99.2% 1372_1386del;2533G>C
3 human 94134 ARHGAP12 Rho GTPase activating prote... NM_001270696.2 96.9% 96.9% 1296_1297ins75;2443G>C
4 human 94134 ARHGAP12 Rho GTPase activating prote... NM_001270699.1 94.3% 94.2% 946_947ins141;2377G>C
5 human 94134 ARHGAP12 Rho GTPase activating prote... XM_005252644.2 94.3% 94.2% 946_947ins141;2377G>C
6 human 94134 ARHGAP12 Rho GTPase activating prote... NM_001270697.1 93.8% 93.7% 946_947ins141;1231_1245del;2392G>C
7 human 94134 ARHGAP12 Rho GTPase activating prote... NM_001270698.2 91.3% 91.3% 946_947ins141;1155_1156ins75;2302G>C
8 human 94134 ARHGAP12 Rho GTPase activating prote... XM_011519761.2 90.6% 90.5% 946_947ins222;1150_1164del;2311G>C
9 human 94134 ARHGAP12 Rho GTPase activating prote... XM_017016954.2 88.1% 88.1% 946_947ins222;1074_1075ins75;2221G>C
10 mouse 75415 Arhgap12 Rho GTPase activating prote... NM_001039692.1 88.7% 91% (many diffs)
11 mouse 75415 Arhgap12 Rho GTPase activating prote... XM_006526314.1 88.2% 90.5% (many diffs)
12 mouse 75415 Arhgap12 Rho GTPase activating prote... XM_011247018.1 86.8% 89.1% (many diffs)
13 mouse 75415 Arhgap12 Rho GTPase activating prote... NM_029277.2 86.4% 89.1% (many diffs)
14 mouse 75415 Arhgap12 Rho GTPase activating prote... XM_017318002.1 83.6% 86.2% (many diffs)
15 mouse 75415 Arhgap12 Rho GTPase activating prote... XM_006526315.1 83.1% 85.6% (many diffs)
16 mouse 75415 Arhgap12 Rho GTPase activating prote... XM_006526316.1 81.3% 84.3% (many diffs)
17 mouse 75415 Arhgap12 Rho GTPase activating prote... XM_017318001.1 74.5% 76.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2592
ORF length:
2523
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gaaaatggct gacagaagtg ggaagattat tccaggacaa gtgtatattg
121aggtggaata tgattatgaa tatgaagcaa aggacagaaa gattgtgata aaacaagggg
181agaggtacat cttggtgaaa aagaccaatg atgactggtg gcaagtcaag ccagatgaaa
241actccaaagc gttttatgtg ccagcccagt atgtgaagga ggtcacgcgc aaagctctca
301tgccacctgt taagcaggta gctggtctgc caaataactc cacgaaaata atgcagagtt
361tgcatcttca gagatcaaca gaaaatgtga acaaattgcc tgagctttca agtttcggaa
421agccatcgtc atctgttcaa ggaacaggtc ttattcgtga tgccaatcag aattttggac
481ccagttataa tcaaggtcag actgtcaacc taagcctgga cctgacccat aataacggaa
541agtttaacaa tgactcacat tctcctaaag tttccagcca gaataggaca cgctcatttg
601gtcattttcc cggtccagag ttcttggatg tagagaaaac tagcttctcc caggaacaat
661cttgtgattc cgcaggagaa ggctctgaaa gaatacatca agattctgaa tctggtgatg
721aacttagcag cagctccact gaacagataa gggcaaccac acctccaaat caaggaaggc
781cagattctcc tgtctatgct aaccttcaag aactgaaaat atcccagtct gctcttcccc
841cacttcctgg gagcccggca attcagatta atggagaatg ggaaactcat aaagacagct
901cagggcgttg ctattactat aacagaggga cacaggaaag aacttggaaa cctcctcgtt
961ggactcggga tgcaagcatc agcaaaggag atttccaaaa tccaggggat caagagcttc
1021tttcatcgga agaaaactac tacagcactt cttacagcca gtcagatagt cagtgtggtt
1081ctcctccaag gggttggtca gaagagttgg atgaacgtgg gcatacctta tataccagtg
1141actatactaa tgaaaagtgg ctcaagcatg ttgatgatca aggtagacaa tattactaca
1201gtgcagacgg atctcggtca gaatgggaat tgccaaagta taatgcttca tcccagcagc
1261aaagagaaat aattaaaagt aggagcctgg acaggcggct gcaagaacca atagtattaa
1321caaagtggag acatagcacc attgtattgg acactaatga taaggaatct ccaactgcct
1381caaaaccctg ctttcctgaa aatgagtctt ctccctcctc accaaagcac caagatacag
1441atcaagagaa atatggatta ttaaatgtaa caaaaattgc tgaaaatggg aaaaaggttc
1501gaaagaactg gttgtcttct tgggcggtgt tgcagggttc atctttactt tttaccaaaa
1561ctcaaggaag tagcacaagt tggtttggca gtaatcagtc caaaccagag ttcacagtgg
1621acctcaaggg ggcaacaatt gagatggctt caaaggataa atccagcaaa aagaatgtat
1681ttgagctgaa aactcgtcaa ggaacagaac tgctaattca gtctgacaat gacactgtta
1741ttaatgattg gtttaaagtt cttagtagta caatcaataa tcaggcagta gaaactgatg
1801aaggaattga agaggagata ccggattcac caggaataga aaagcatgat aaagaaaagg
1861aacaaaagga tcccaaaaag cttcgttcct ttaaagtatc tagcatagat tcttcagaac
1921agaaaaaaac caagaaaaac ttaaagaagt ttcttacacg acgccccact ttgcaagctg
1981ttcgtgaaaa aggttatatt aaagatcagg tatttggatc caatctcgct aatctgtgtc
2041agagagagaa tggcacagta ccaaagtttg tgaagttatg tattgaacat gttgaagaac
2101atggtttgga tattgatggg atatacagag taagtggcaa cctcgcagtg atccagaaac
2161taaggtttgc agtcaatcaT GATGAGAAAT TGGACTTGAA TGACAGTAAA TGGGAAGATA
2221TTCATGTCAT TACTGGAGCC CTCAAAATGT TTTTTCGAGA ATTACCAGAA CCTCTTTTTA
2281CATTTAATCA TTTTAATGAT TTTGTTAATG CAATTAAGCA AGAACCAAGA CAGCGAGTCG
2341CTGCTGTTAA GGACCTAATC AGACAGTTGC CAAAGCCAAA CCAAGACACA ATGCAGATTC
2401TTTTCCGACA TCTCAGAAGA GTTATAGAAA ATGGAGAGAA AAATCGAATG ACCTATCAGA
2461GTATAGCAAT TGTTTTTGGT CCCACTCTAT TAAAACCAGA AAAAGAGACT GGTAATATAG
2521CAGTTCATAC TGTGTACCAG AATCAGATTG TAGAATTAAT TCTTCTGGAA CTGAGTTCCA
2581TCTTCCGACG TTTGCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA
2641ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT
2701CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAACA TATATAATGG TCGTCTGCCT
2761CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t

Download FASTA (ORF) (Full)