Transcript: Human XM_005252644.2

PREDICTED: Homo sapiens Rho GTPase activating protein 12 (ARHGAP12), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
ARHGAP12 (94134)
Length:
4902
CDS:
297..2681

Additional Resources:

NCBI RefSeq record:
XM_005252644.2
NBCI Gene record:
ARHGAP12 (94134)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_005252644.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000047266 GCACCATTGTATTGGACACTA pLKO.1 1423 CDS 100% 4.950 6.930 N ARHGAP12 n/a
2 TRCN0000291761 GCACCATTGTATTGGACACTA pLKO_005 1423 CDS 100% 4.950 6.930 N ARHGAP12 n/a
3 TRCN0000047267 CCTGACCCATAATAACGGAAA pLKO.1 749 CDS 100% 4.050 5.670 N ARHGAP12 n/a
4 TRCN0000291762 CCTGACCCATAATAACGGAAA pLKO_005 749 CDS 100% 4.050 5.670 N ARHGAP12 n/a
5 TRCN0000375710 AGTCATATACACTGGATAATT pLKO_005 2968 3UTR 100% 15.000 10.500 N Arhgap12 n/a
6 TRCN0000047264 CGCTGCTGTTAAGGACCTAAT pLKO.1 2426 CDS 100% 10.800 7.560 N ARHGAP12 n/a
7 TRCN0000291833 CGCTGCTGTTAAGGACCTAAT pLKO_005 2426 CDS 100% 10.800 7.560 N ARHGAP12 n/a
8 TRCN0000047265 CCCAGGAACAATCTTGTGATT pLKO.1 877 CDS 100% 4.950 3.465 N ARHGAP12 n/a
9 TRCN0000047263 CCAGTCATATACACTGGATAA pLKO.1 2966 3UTR 100% 1.080 0.756 N ARHGAP12 n/a
10 TRCN0000291831 CCAGTCATATACACTGGATAA pLKO_005 2966 3UTR 100% 1.080 0.756 N ARHGAP12 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_005252644.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13005 pDONR223 100% 99.3% 99.3% None 1230_1231insGCCAGCAGTCCAAAG n/a
2 ccsbBroad304_13005 pLX_304 0% 99.3% 99.3% V5 1230_1231insGCCAGCAGTCCAAAG n/a
3 ccsbBroadEn_13006 pDONR223 100% 94.4% 94.4% None 946_947ins141 n/a
4 ccsbBroad304_13006 pLX_304 0% 94.4% 94.4% V5 946_947ins141 n/a
5 TRCN0000477925 ACATATATAATGGTCGTCTGCCTC pLX_317 16.6% 94.3% 94.2% V5 946_947ins141;2377G>C n/a
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