Transcript: Mouse XM_006526314.1

PREDICTED: Mus musculus Rho GTPase activating protein 12 (Arhgap12), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Arhgap12 (75415)
Length:
5040
CDS:
401..2932

Additional Resources:

NCBI RefSeq record:
XM_006526314.1
NBCI Gene record:
Arhgap12 (75415)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006526314.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000306653 GGCCGTTGTTACTACTATAAC pLKO_005 1229 CDS 100% 13.200 18.480 N Arhgap12 n/a
2 TRCN0000105722 GCCAGAATAGGACACGCTTAT pLKO.1 903 CDS 100% 10.800 15.120 N Arhgap12 n/a
3 TRCN0000105723 GCGTATATTGAGGTGGAGTAT pLKO.1 437 CDS 100% 4.950 6.930 N Arhgap12 n/a
4 TRCN0000375710 AGTCATATACACTGGATAATT pLKO_005 3192 3UTR 100% 15.000 10.500 N Arhgap12 n/a
5 TRCN0000306654 GAAACCTGTGTACTGCATATT pLKO_005 3384 3UTR 100% 13.200 9.240 N Arhgap12 n/a
6 TRCN0000306652 GTTCGTGAGAAAGGTTATATC pLKO_005 2318 CDS 100% 13.200 9.240 N Arhgap12 n/a
7 TRCN0000105720 CCACCCTTCTAAAGCCAGAAA pLKO.1 2820 CDS 100% 4.950 3.465 N Arhgap12 n/a
8 TRCN0000327237 CCACCCTTCTAAAGCCAGAAA pLKO_005 2820 CDS 100% 4.950 3.465 N Arhgap12 n/a
9 TRCN0000105724 CCAGTGACTATACTAAGGAAA pLKO.1 1458 CDS 100% 4.950 3.465 N Arhgap12 n/a
10 TRCN0000105721 CCTGACCCATAATAATGGAAA pLKO.1 847 CDS 100% 4.950 3.465 N Arhgap12 n/a
11 TRCN0000047263 CCAGTCATATACACTGGATAA pLKO.1 3190 3UTR 100% 1.080 0.756 N ARHGAP12 n/a
12 TRCN0000291831 CCAGTCATATACACTGGATAA pLKO_005 3190 3UTR 100% 1.080 0.756 N ARHGAP12 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006526314.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13006 pDONR223 100% 88.2% 90.6% None (many diffs) n/a
2 ccsbBroad304_13006 pLX_304 0% 88.2% 90.6% V5 (many diffs) n/a
3 TRCN0000477925 ACATATATAATGGTCGTCTGCCTC pLX_317 16.6% 88.2% 90.5% V5 (many diffs) n/a
4 ccsbBroadEn_13005 pDONR223 100% 83.6% 86.1% None (many diffs) n/a
5 ccsbBroad304_13005 pLX_304 0% 83.6% 86.1% V5 (many diffs) n/a
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