Construct: ORF TRCN0000477947

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF007043.1_s317c1
Derived from:
ccsbBroadEn_03389
DNA Barcode:
TAACCAACGCGGCAACTAACCGGT
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TPCN1 (53373)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000477947
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 53373 TPCN1 two pore segment channel 1 NM_017901.6 100% 100%
2 human 53373 TPCN1 two pore segment channel 1 XM_011538492.2 100% 100%
3 human 53373 TPCN1 two pore segment channel 1 NM_001143819.3 91.8% 91.8% 1_216del
4 human 53373 TPCN1 two pore segment channel 1 XM_011538490.2 91.8% 91.8% 1_216del
5 human 53373 TPCN1 two pore segment channel 1 NM_001301214.1 91.6% 91.6% 0_1ins204
6 human 53373 TPCN1 two pore segment channel 1 NM_001351346.2 91.6% 91.6% 0_1ins204
7 human 53373 TPCN1 two pore segment channel 1 NM_001351347.1 91.6% 91.6% 0_1ins204
8 human 53373 TPCN1 two pore segment channel 1 XM_011538493.2 91.6% 91.6% 0_1ins204
9 human 53373 TPCN1 two pore segment channel 1 XM_017019480.2 89.8% 89.7% 1_216del;1615_1616ins54
10 human 53373 TPCN1 two pore segment channel 1 XR_001748766.2 64.7% (many diffs)
11 human 53373 TPCN1 two pore segment channel 1 XM_017019482.2 64% 63.9% 1_216del;1923_1923delGins742
12 human 53373 TPCN1 two pore segment channel 1 XR_001748767.2 63% (many diffs)
13 human 53373 TPCN1 two pore segment channel 1 XR_001748765.2 44.4% 1_385del;2089_2249del;2995_5510del
14 mouse 252972 Tpcn1 two pore channel 1 NM_145853.2 86.8% 90.9% (many diffs)
15 mouse 252972 Tpcn1 two pore channel 1 XM_011248201.2 86.8% 90.9% (many diffs)
16 mouse 252972 Tpcn1 two pore channel 1 XR_387565.3 59.1% (many diffs)
17 mouse 252972 Tpcn1 two pore channel 1 XR_387566.3 44.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2517
ORF length:
2448
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggctgtgagt ttggatgacg acgtgccgct catcctgacc ttggatgagg
121gtggcagtgc cccactggct ccctccaacg gcctgggcca agaagagcta cctagcaaaa
181atggcggcag ctatgccatc cacgactccc aggcccccag tctcagctct gggggtgaga
241gttccccctc cagccccgca cacaactggg agatgaatta ccaagaggca gcaatctacc
301tccaggaagg cgagaacaac gacaagttct tcacccaccc caaggatgcc aaggcgctgg
361cggcctacct ctttgcacac aatcacctct tctacctgat ggagctggcc acggccctgc
421tgctgctgct gctctccctg tgcgaggccc ccgccgtccc cgcactccgg cttggcatct
481atgtccacgc caccctggag ctgtttgccc tgatggtggt agtgtttgaa ctctgcatga
541agttacgctg gctgggcctc cacaccttca tccggcacaa gcggaccatg gtcaagacct
601cggtgctggt ggtgcagttt gtcgaggcca tcgtggtgtt ggtacggcag atgtcccatg
661tgcgggtgac ccgagcactg cgctgcattt tcctggtgga ctgtcggtat tgcggtggcg
721tccggcgcaa cctgcggcag atcttccagt ccctgccgcc cttcatggac atcctcctgc
781tgctgctgtt cttcatgatc atctttgcca tcctcggttt ctacttgttc tcccctaacc
841cttcagaccc ctacttcagc accctggaga acagcatcgt cagtctgttt gtccttctga
901ccacagccaa tttcccagat gtgatgatgc cctcctactc ccggaacccc tggtcctgcg
961tcttcttcat cgtgtacctc tccatcgagc tgtatttcat catgaacctg cttctggctg
1021tggtgttcga caccttcaat gacattgaga aacgcaagtt caagtctttg ctactgcaca
1081agcgaaccgc tatccagcat gcctaccgcc tgctcatcag ccagaggagg cctgccggca
1141tctcctacag gcagtttgaa ggcctcatgc gcttctacaa gccccggatg agtgccaggg
1201agcgctatct taccttcaag gccctgaatc agaacaacac acccctgctc agcctaaagg
1261acttttacga tatctacgaa gttgctgctt tgaagtggaa ggccaagaaa aacagagagc
1321actggtttga tgagcttccc aggacggcgc tcctcatctt caaaggtatt aatatccttg
1381tgaagtccaa ggccttccag tatttcatgt acttggtggt ggcagtcaac ggggtctgga
1441tcctcgtgga gacatttatg ctgaaaggtg ggaacttctt ctccaagcac gtgccctgga
1501gttacctcgt ctttctaact atctatgggg tggagctgtt cctgaaggtt gccggcctgg
1561gccctgtgga gtacttgtct tccggatgga acttgtttga cttctccgtg acagtgttcg
1621ccttcctggg actgctggcg ctggccctca acatggagcc cttctatttc atcgtggtcc
1681tgcgccccct ccagctgctg aggttgttta agttgaagga gcgctaccgc aacgtgctgg
1741acaccatgtt cgagctgctg ccccggatgg ccagcctggg cctcaccctg ctcatctttt
1801actactcctt cgccatcgtg ggcatggagt tcttctgcgg gatcgtcttc cccaactgct
1861gcaacacgag tacagtggca gatgcctacc gctggcgcaa ccacaccgtg ggcaacagga
1921ccgtggtgga ggaaggctac tattatctca ataattttga caacatcctc aacagctttg
1981tgaccctgtt tgagctcaca gttgtcaaca actggtacat catcatggaa ggcgtcacct
2041ctcagacctc ccactggagc cgcctctact tcatgacctt ttacattgtg accatggtgg
2101tgatgacgat cattgtcgcc tttatcctcg aggccttcgt cttccgaatg aactacagcc
2161gcaagaacca ggactcggaa gttgatggtg gcatcaccct tgagaaggaa atctccaaag
2221aagagctggt tgccgtcctg gagctctacc gggaggcacg gggggcctcc tcggatgtca
2281ccaggctgct ggagaccctc tcccagatgg agagatacca gcaacattcc atggtgtttc
2341TGGGACGGCG ATCAAGGACC AAGAGCGACC TGAGCCTGAA GATGTACCAG GAGGAGATCC
2401AGGAGTGGTA TGAGGAGCAT GCCAGGGAGC AAGAGCAGCA GCGACAACTC AGCAGCAGTG
2461CAGCCCCCGC CGCCCAGCAG CCCCCAGGCA GCCGCCAGCG CTCCCAGACC GTTACCTTTG
2521CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT
2581CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT
2641TATATATCTT GTGGAAAGGA CGATAACCAA CGCGGCAACT AACCGGTACG CGTTAAGTCg
2701acaatcaacc tctggattac aaaatttgtg aaagatt

Download FASTA (ORF) (Full)