Construct: ORF TRCN0000477950
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004634.1_s317c1
- Derived from:
- ccsbBroadEn_00948
- DNA Barcode:
- CTAATTATTTTGGGTCCGCAGTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LOX (4015)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477950
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4015 | LOX | lysyl oxidase | NM_002317.7 | 100% | 100% | |
| 2 | human | 4015 | LOX | lysyl oxidase | NM_001178102.2 | 44.8% | 44.8% | 0_1ins690 |
| 3 | human | 4015 | LOX | lysyl oxidase | NM_001317073.1 | 28.7% | 28.7% | 0_1ins891 |
| 4 | mouse | 16948 | Lox | lysyl oxidase | NM_001286181.1 | 83.6% | 86.3% | (many diffs) |
| 5 | mouse | 16948 | Lox | lysyl oxidase | NM_001286182.1 | 83.6% | 86.3% | (many diffs) |
| 6 | mouse | 16948 | Lox | lysyl oxidase | NM_010728.3 | 83.6% | 86.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1320
- ORF length:
- 1251
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcgcttcgcc tggaccgtgc tcctgctcgg gcctttgcag ctctgcgcgc 121 tagtgcactg cgcccctccc gccgccggcc aacagcagcc cccgcgcgag ccgccggcgg 181 ctccgggcgc ctggcgccag cagatccaat gggagaacaa cgggcaggtg ttcagcttgc 241 tgagcctggg ctcacagtac cagcctcagc gccgccggga cccgggcgcc gccgtccctg 301 gtgcagccaa cgcctccgcc cagcagcccc gcactccgat cctgctgatc cgcgacaacc 361 gcaccgccgc ggcgcgaacg cggacggccg gctcatctgg agtcaccgct ggccgcccca 421 ggcccaccgc ccgtcactgg ttccaagctg gctactcgac atctagagcc cgcgaagctg 481 gcgcctcgcg cgcggagaac cagacagcgc cgggagaagt tcctgcgctc agtaacctgc 541 ggccgcccag ccgcgtggac ggcatggtgg gcgacgaccc ttacaacccc tacaagtact 601 ctgacgacaa cccttattac aactactacg atacttatga aaggcccaga cctgggggca 661 ggtaccggcc cggatacggc actggctact tccagtacgg tctcccagac ctggtggccg 721 acccctacta catccaggcg tccacgtacg tgcagaagat gtccatgtac aacctgagat 781 gcgcggcgga ggaaaactgt ctggccagta cagcatacag ggcagatgtc agagattatg 841 atcacagggt gctgcTCAGA TTTCCCCAAA GAGTGAAAAA CCAAGGGACA TCAGATTTCT 901 TACCCAGCCG ACCAAGATAT TCCTGGGAAT GGCACAGTTG TCATCAACAT TACCACAGTA 961 TGGATGAGTT TAGCCACTAT GACCTGCTTG ATGCCAACAC CCAGAGGAGA GTGGCTGAAG 1021 GCCACAAAGC AAGTTTCTGT CTTGAAGACA CATCCTGTGA CTATGGCTAC CACAGGCGAT 1081 TTGCATGTAC TGCACACACA CAGGGATTGA GTCCTGGCTG TTATGATACC TATGGTGCAG 1141 ACATAGACTG CCAGTGGATT GATATTACAG ATGTAAAACC TGGAAACTAT ATCCTAAAGG 1201 TCAGTGTAAA CCCCAGCTAC CTGGTTCCTG AATCTGACTA TACCAACAAT GTTGTGCGCT 1261 GTGACATTCG CTACACAGGA CATCATGCGT ATGCCTCAGG CTGCACAATT TCACCGTATT 1321 TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1381 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1441 TTTATATATC TTGTGGAAAG GACGACTAAT TATTTTGGGT CCGCAGTTCA CGCGTTAAGT 1501 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt