Construct: ORF TRCN0000477957
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015621.1_s317c1
- Derived from:
- ccsbBroadEn_04586
- DNA Barcode:
- TACTGTATTATCAGGTGATGGGAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMEM169 (92691)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477957
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 92691 | TMEM169 | transmembrane protein 169 | NM_001142310.1 | 100% | 100% | |
| 2 | human | 92691 | TMEM169 | transmembrane protein 169 | NM_001142311.2 | 100% | 100% | |
| 3 | human | 92691 | TMEM169 | transmembrane protein 169 | NM_001142312.2 | 100% | 100% | |
| 4 | human | 92691 | TMEM169 | transmembrane protein 169 | NM_138390.4 | 100% | 100% | |
| 5 | human | 92691 | TMEM169 | transmembrane protein 169 | XM_006712851.1 | 100% | 100% | |
| 6 | human | 92691 | TMEM169 | transmembrane protein 169 | XM_011512173.2 | 100% | 100% | |
| 7 | mouse | 271711 | Tmem169 | transmembrane protein 169 | NM_175564.4 | 85.5% | 87.8% | (many diffs) |
| 8 | mouse | 271711 | Tmem169 | transmembrane protein 169 | XM_006496051.1 | 85.5% | 87.8% | (many diffs) |
| 9 | mouse | 271711 | Tmem169 | transmembrane protein 169 | XM_006496052.2 | 85.5% | 87.8% | (many diffs) |
| 10 | mouse | 271711 | Tmem169 | transmembrane protein 169 | XM_006496053.2 | 85.5% | 87.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 957
- ORF length:
- 891
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga agagccaaca gcagtagaag gccaggtcca gcttccaagc ccccaccagg 121 gctctctcag gaaggctgtg gctgctgccc tggcgctgga tggggaatcc acaatggggc 181 acaggaaaaa gaagaggaaa gagtcacgcc cagaatccat catcatctac cgctcagaca 241 atgagaaaac agatgaggag cccggagaat cagaaggtgg agatcagcct aaagaggagg 301 agggagatga tttcctagac tatcctgtgg atgatgatat gtggaacctg cctctggaca 361 gccgctacgt caccttaact gggaccatca cacgagggaa gaaaaagggt cagatggtgg 421 acatccatgt cacattgaca gagaaagagc tgcaggaact gaccaaacct aaagagtcat 481 caagggaaac gacgcctgaa ggaagaatgg cctgccagat gggagctgac cgtgggcccc 541 atgtggtcct ctggacgctg atctgcctgc ctgtggtttt catcctttct tttgttgtct 601 ctttctacta cggcactatc acctggtaca acatcttcct cgtgtataat gaggaaagga 661 ccttctggca caagatctcg tattgccctt gcctcgttct cttctatcca gtgctcatca 721 tggccatggc ttcttccctc ggcctctacg ctgctgtggt ccagctctcg tggtcctggg 781 aagcatggtg gcaagctgcc cgggacatgg agaaaggctt ctgtggctgg ctcTGCAGCA 841 AGCTGGGTCT GGAGGACTGT TCTCCCTACA GCATTGTGGA GTTGCTTGAA TCCGACAATA 901 TCTCAAGCAC TCTCTCCAAC AAGGACCCCA TCCAAGAAGT AGAAACCTCC ACGGTCTACC 961 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1021 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1081 ATATATCTTG TGGAAAGGAC GATACTGTAT TATCAGGTGA TGGGAGACGC GTTAAGTCga 1141 caatcaacct ctggattaca aaatttgtga aagatt