Construct: ORF TRCN0000477967
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011806.1_s317c1
- Derived from:
- ccsbBroadEn_07901
- DNA Barcode:
- GTGCGGCTGTAGGATGTCGGGCCA
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TSPAN15 (23555)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477967
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23555 | TSPAN15 | tetraspanin 15 | NM_012339.5 | 99.6% | 98.6% | 819G>T;838A>G;871delT |
| 2 | human | 23555 | TSPAN15 | tetraspanin 15 | XM_017016010.1 | 73.1% | 46.6% | (many diffs) |
| 3 | human | 23555 | TSPAN15 | tetraspanin 15 | NM_001351263.1 | 70% | 69% | (many diffs) |
| 4 | human | 23555 | TSPAN15 | tetraspanin 15 | XM_005269666.4 | 69.1% | 57.1% | (many diffs) |
| 5 | human | 23555 | TSPAN15 | tetraspanin 15 | XR_001747074.1 | 68% | (many diffs) | |
| 6 | human | 23555 | TSPAN15 | tetraspanin 15 | XM_011539562.2 | 59.9% | 58.1% | (many diffs) |
| 7 | human | 23555 | TSPAN15 | tetraspanin 15 | XM_011539563.2 | 50.6% | 49.6% | (many diffs) |
| 8 | human | 23555 | TSPAN15 | tetraspanin 15 | NR_147091.1 | 49.2% | (many diffs) | |
| 9 | human | 23555 | TSPAN15 | tetraspanin 15 | XR_001747073.1 | 28.9% | (many diffs) | |
| 10 | human | 23555 | TSPAN15 | tetraspanin 15 | XR_001747072.1 | 10.7% | (many diffs) | |
| 11 | mouse | 70423 | Tspan15 | tetraspanin 15 | NM_197996.2 | 87.7% | 91.1% | (many diffs) |
| 12 | mouse | 70423 | Tspan15 | tetraspanin 15 | XM_006514080.1 | 65.5% | 67.3% | (many diffs) |
| 13 | mouse | 70423 | Tspan15 | tetraspanin 15 | XM_011243561.1 | 57.3% | 54.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 948
- ORF length:
- 882
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc gcgcggggac tcggagcagg tgcgctactg cgcgcgcttc tcctacctct 121 ggctcaagtt ttcacttatc atctattcca ccgtgttctg gctgattggg gccctggtcc 181 tgtctgtggg catctatgca gaggttgagc ggcagaaata taaaaccctt gaaagtgcct 241 tcctggctcc agccatcatc ctcatcctcc tgggcgtcgt catgttcatg gtctccttca 301 ttggtgtgct ggcgtccctc cgtgacaacc tgtaccttct ccaagcattc atgtacatcc 361 ttgggatctg cctcatcatg gagctcattg gtggcgtggt ggccttgacc ttccggaacc 421 agaccattga cttcctGAAC GACAACATTC GAAGAGGAAT TGAGAACTAC TATGATGATC 481 TGGACTTCAA AAACATCATG GACTTTGTTC AGAAAAAGTT CAAGTGCTGT GGCGGGGAGG 541 ACTACCGAGA TTGGAGCAAG AATCAGTACC ACGACTGCAG TGCCCCTGGA CCCCTGGCCT 601 GTGGGGTGCC CTACACCTGC TGCATCAGGA ACACGACAGA AGTTGTCAAC ACCATGTGTG 661 GCTACAAAAC TATCGACAAG GAGCGTTTCA GTGTGCAGGA TGTCATCTAC GTGCGGGGCT 721 GCACCAACGC CGTGATCATC TGGTTCATGG ACAACTACAC CATCATGGCG GGCATCCTCC 781 TGGGCATCCT GCTTCCCCAG TTCCTGGGGG TGCTGCTGAC GCTGCTGTAC ATCACCCGGG 841 TGGAGGACAT CATCATGGAG CACTCTGTCA CTGATGGGCT CCTTGGGCCC GGTGCCAAGC 901 CCGGCGTGGA GGCGGCAGGC ACGGGATGCT GCTTGGCTAC CCCAATTACC CAACTTTCTT 961 GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC 1021 GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG 1081 TGGAAAGGAC GAGTGCGGCT GTAGGATGTC GGGCCAACGC GTTAAGTCga caatcaacct 1141 ctggattaca aaatttgtga aagatt