Construct: ORF TRCN0000477969
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006050.1_s317c1
- Derived from:
- ccsbBroadEn_08749
- DNA Barcode:
- CAAAGACCCGGTAGCAACATAGAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- STIM2 (57620)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477969
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57620 | STIM2 | stromal interaction molecule 2 | NM_001169117.2 | 87.2% | 87.3% | 0_1ins261;798G>A |
| 2 | human | 57620 | STIM2 | stromal interaction molecule 2 | NM_020860.4 | 70.6% | 70.8% | (many diffs) |
| 3 | human | 57620 | STIM2 | stromal interaction molecule 2 | NM_001169118.2 | 70% | 70.1% | (many diffs) |
| 4 | mouse | 116873 | Stim2 | stromal interaction molecule 2 | XM_006503682.3 | 66.8% | 70.6% | (many diffs) |
| 5 | mouse | 116873 | Stim2 | stromal interaction molecule 2 | NM_001081103.2 | 61.6% | 66.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2127
- ORF length:
- 2058
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaacgcagcc gggatcagag ctccggaggc cgccggtgcc gatgggacca 121 ggctggcgcc cggcgggagc ccgtgtctga ggcggcgggg gcggccggag gagtcgccgg 181 cggcggtggt ggcgcctcgc ggagccggcg agctgcaggc ggccggggcg ccgctgcgct 241 ttcacccggc ttctcctcgg cgccttcatc ccgcctcgac tcctggccca gcgtggggct 301 ggctgctgcg gcggcggcgc tgggctgcgt tgctggtgct cgggctgctg gtagccggag 361 cggcggacgg atgcgagctt gtgccccggc acctccgcgg gcggcgggcg actggctctg 421 ccgcaactgc cgcctcctct cccgccgcgg cggccggcga tagcccggcg ctcatgacag 481 atccctgcat gtcactgagt ccaccatgct ttacagaaga agacagattt agtctggaag 541 ctcttcaaac aatacataaa caaatggatg atgacaaaga tggtggaatt gaagtagagg 601 aaagtgatga attcatcaga gaagatatga aatataaaga tgctactaat aaacacagcc 661 atctgcacag agaagataaa catataacga ttgaggattt atggaaacga tggaaaacat 721 cagaagttca taattggacc cttgaagaca ctcttcagtg gttgatagag tttgttgaac 781 taccccaata tgagaagaat tttagagaca acaatgtcaa aggaacgaca cttcccagga 841 tagcagtgca cgaaccttca tttatgatct cccagttgaa aatcagtgac cggagtcaca 901 gacaaaaact tcagctcaag gcattggatg tggttttgtt tggacctcta acacgcccac 961 ctcataactg gatgaaagat tttatcctca cagtttctat agtaattggt gttggaggct 1021 gctggtttgc ttatacgcag aataagacat caaaagaaca tgttgcaaaa atgatgaaag 1081 atttagagag cttacaaact gcagagcaaa gtctaatgga cttacaagag aggcttgaaa 1141 aggcacagga agaaaacaga aatgttgctg tagaaaagca aaatttagag cgcaaaatga 1201 tggatgaaat caattatgca aaggaggagg cttgtcggct gagagagcta agggagggag 1261 ctgaatgtga attgagtaga cgtcagtatg cagaacagga attggaacag gttcgcatgg 1321 ctctgaaaaa ggccgaaaaa gaatttgaac tgagaagcag ttggtctgtt ccagatgcac 1381 ttcagaaatg gcttcagtta acacatgaag tagaagtgca atactacaat attaaaagac 1441 aaaacgctga aatgcagcta gctattgcta aagatgaggc agaaaaaatt aaaaagaaga 1501 gaagcacagt ctttgggact ctgcacgttg cacacagctc ctccctagat gaggtagacc 1561 acaaaattct ggaagcaaag aaagctctct ctgagttgac aacttgttta cgagaacgac 1621 tttttcgctg gcaacaaatt gagaagatct gtggctttca gatagcccat aactcaggac 1681 tccccagcct gacctcttcc ctttattctg aTCACAGCTG GGTGGTGATG CCCAGAGTCT 1741 CCATTCCACC CTATCCAATT GCTGGAGGAG TTGATGACTT AGATGAAGAC ACACCCCCAA 1801 TAGTGTCACA ATTTCCCGGG ACCATGGCTA AACCTCCTGG ATCATTAGCC AGAAGCAGCA 1861 GCCTGTGCCG TTCACGCCGC AGCATTGTGC CGTCCTCGCC TCAGCCTCAG CGAGCTCAGC 1921 TTGCTCCACA CGCCCCCCAC CCGTCACACC CTCGGCACCC TCACCACCCG CAACACACAC 1981 CACACTCCTT GCCTTCCCCT GATCCAGATA TCCTCTCAGT GTCAAGTTGC CCTGCGCTTT 2041 ATCGAAATGA AGAGGAGGAA GAGGCCATTT ACTTCTCTGC TGAAAAGCAA TGTATTCACC 2101 TTGGGCTCGG TGCGTGCAAA AGTGAATTGC CAACTTTCTT GTACAAAGTG GTTGATATCG 2161 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 2221 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GACAAAGACC 2281 CGGTAGCAAC ATAGATACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 2341 aagatt