Construct: ORF TRCN0000477975
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003439.1_s317c1
- Derived from:
- ccsbBroadEn_10513
- DNA Barcode:
- CCAATGGATACCGTATTGCATTCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RPL23AP32 (56969)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477975
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6147 | RPL23A | ribosomal protein L23a | NM_000984.6 | 77.1% | 71.1% | (many diffs) |
| 2 | human | 56969 | RPL23AP32 | ribosomal protein L23a pseu... | NR_002229.2 | 62.7% | 1_124del;382C>T;515_620del | |
| 3 | human | 285830 | HLA-F-AS1 | HLA-F antisense RNA 1 | NR_026973.1 | 49.9% | (many diffs) | |
| 4 | human | 649946 | RPL23AP64 | ribosomal protein L23a pseu... | NR_003040.2 | 47.4% | (many diffs) | |
| 5 | human | 118433 | RPL23AP7 | ribosomal protein L23a pseu... | NR_000029.3 | 28.4% | (many diffs) | |
| 6 | human | 118433 | RPL23AP7 | ribosomal protein L23a pseu... | NR_024529.1 | 25.2% | (many diffs) | |
| 7 | human | 284942 | RPL23AP82 | ribosomal protein L23a pseu... | NR_026982.1 | 24.2% | (many diffs) | |
| 8 | human | 118433 | RPL23AP7 | ribosomal protein L23a pseu... | NR_024528.1 | 23.6% | (many diffs) | |
| 9 | human | 284942 | RPL23AP82 | ribosomal protein L23a pseu... | NR_026981.1 | 23% | (many diffs) | |
| 10 | human | 285830 | HLA-F-AS1 | HLA-F antisense RNA 1 | NR_026972.1 | 18.2% | (many diffs) | |
| 11 | human | 388574 | RPL23AP87 | ribosomal protein L23a pseu... | NR_029406.1 | 15.4% | (many diffs) | |
| 12 | human | 644128 | RPL23AP53 | ribosomal protein L23a pseu... | NR_003572.2 | 5.8% | (many diffs) | |
| 13 | mouse | 268449 | Rpl23a | ribosomal protein L23A | NM_207523.2 | 71.7% | 71.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 70
- ORF end:
- 460
- ORF length:
- 390
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgaTC AACAAGTTTG TACAAAAAAA 61 GTTGGCACCA TGGCACCGAA AGTGAAGAAG GAAGCTCCTG GCCCGCCTAA AGCTGAAGCC 121 AAAGCAAAGG CTTTAAAGGC CAAGAAGGTA GTGTTGAAAG GTGTCCACGG CCACAAAAAA 181 AAGAAGATCC GCATGTCACC CACCTTCCAG CGGCCCAAGA CACTGAGACT CTGGAGGCCG 241 CCCAGATATC CTCGGAAGAC CACCCCCAGG AGAAACAAGC TTGACCACTA TGCTATCATC 301 AAGTTTCCTC TGACCACTGA GTTTGCTATG AAGAAGATAA AAGACAACAA CACCCTTGTG 361 TTCACTGTGG ATGTTAAAGC CAACAAGCAC CAGATCAAAC AGGCTGTGAA GAAGCTCTGT 421 GACATTGATG GGGCCAAGGT CAACACCCTG ATGGAGAGAT TGCCAACTTT CTTGTACAAA 481 GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA 541 TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG 601 GACGACCAAT GGATACCGTA TTGCATTCAA CGCGTTAAGT Cgacaatcaa cctctggatt 661 acaaaatttg tgaaagatt