Construct: ORF TRCN0000477987
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005625.1_s317c1
- Derived from:
- ccsbBroadEn_07857
- DNA Barcode:
- ACGAAAGATTAGGCACAAGGACTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RHOBTB2 (23221)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477987
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23221 | RHOBTB2 | Rho related BTB domain cont... | NM_015178.2 | 99.9% | 100% | 864T>C |
| 2 | human | 23221 | RHOBTB2 | Rho related BTB domain cont... | NM_001160037.1 | 99% | 99% | 1_21del;885T>C |
| 3 | human | 23221 | RHOBTB2 | Rho related BTB domain cont... | NM_001160036.2 | 97% | 97% | 1_66del;930T>C |
| 4 | human | 23221 | RHOBTB2 | Rho related BTB domain cont... | XM_017013251.1 | 97% | 97% | 1_66del;930T>C |
| 5 | mouse | 246710 | Rhobtb2 | Rho-related BTB domain cont... | NM_153514.5 | 90.2% | 97.5% | (many diffs) |
| 6 | mouse | 246710 | Rhobtb2 | Rho-related BTB domain cont... | XM_017316022.1 | 87.6% | 94.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2247
- ORF length:
- 2181
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ttctgacatg gattatgaaa ggccaaacgt agagaccatc aagtgcgttg 121 tggtggggga caacgccgtg ggtaagacca ggctcatctg tgcccgcgct tgcaatgcca 181 ccctcaccca gtaccagctg ctggccacgc atgtgcccac agtatgggcc atcgaccaat 241 atcgtgtgtg ccaggaggtg ctggaacgct cccgagacgt ggtagatgat gtcagcgtct 301 ctctgcgcct ctgggacacc tttggagacc accacaaaga ccgtcgcttt gcttatggga 361 gatctgatgt ggtggttctg tgcttctcca ttgccaaccc caattccctc caccatgtca 421 agaccatgtg gtacccagaa atcaagcact tctgcccccg agcacctgtc atcttggtgg 481 gctgccagtt ggacctgcgc tacgctgacc tggaggctgt caacagggct aggcgaccct 541 tggctaggcc catcaaacct aatgaaatcc tgcccccaga gaagggtcgg gaggtggcca 601 aggagctggg catcccctac tatgagacca gcgtggtggc ccagttcggc atcaaggacg 661 tctttgacaa cgccatccga gctgcactca tctcccgccg ccacctgcag ttctggaagt 721 cccacctccg caatgtgcag cggcctctgc tgcaggcacc cttcctaccc cccaagccac 781 cgcccccgat catcgtggtg cccgaccctc cctccagcag cgaggagtgc cccgcccacc 841 tcctggagga cccgctctgc gcggacgtca tcctggtgct gcaggagcgg gtgcgcatct 901 ttgcccacaa gatctacctc tccacctcct cctccaagtt ctatgacctg ttcctcatgg 961 acctgagtga gggggagctg gggggcccct cggagccagg gggcacccac ccagaggacc 1021 accagggcca ctctgatcaa caccaccacc atcaccacca ccaccatggg cgagacttcc 1081 tgctccgagc agccagcttt gacgtgtgcg agagcgtgga tgaggctggg ggctccggtc 1141 ctgctggcct ccgtgcttcc accagcgacg ggatcttacg gggcaacgga acagggtacc 1201 taccgggcag gggtcgtgtg ctgtcttcct ggagccgagc ttttgtgagc atccaggaag 1261 agatggcaga agatcctctc acctacaaat cccggctgat ggtggtggtg aagatggaca 1321 gttccatcca gccggggccc ttccgggctg tcctcaagta cctgtacacg ggggagctag 1381 atgagaacga gcgtgacctc atgcacattg cccacattgc tgagctgctc gaggtctttg 1441 atctgcgcat gatggtggcc aacattctca acaatgaggc cttcatgaac caggagatca 1501 ccaaggcctt ccacgtccgc cggaccaacc gggttaagga gtgcttggca aaaggcacct 1561 tctcagatgt gaccttcatc ctggatgatg gcaccatcag cgcccacaag cccctgttga 1621 tttccagctg tgactggatg gctgccatgt ttggggggcc atttgtggag agctccaccc 1681 gggaggtggt gtttccctac acaagcaaga gctgcatgcg ggccgtgctg gaatacctct 1741 acaccggcat gttcacctcc agccccgacc tggatgacat gaagctcatc attctagcca 1801 accgcctctg cctgccacac ctggttgccc tcacagagca gtacacagtg accgggctga 1861 tggaagcgac ccagatgatg gtggacatcg atggggacgt ccttgtgttc ctggaactgg 1921 ctcagttcca ctgtgcgtac cagctggccg actggtgtct ccaccacatc tgcaccaact 1981 acaacaacgt gtgccgcaag ttcccccgag acatgaaggc catgtcccca gaaaaccagg 2041 agtatttcga gaagcatcgg tggccacctg tgtggtacct gaaggaggaa gatcattacc 2101 agcGGGCACG GAAGGAGCGT GAGAAGGAGG ACTACCTCCA CCTCAAGCGG CAGCCCAAAC 2161 GGCGTTGGCT CTTCTGGAAC AGTCCATCCT CCCCGTCTTC CTCGGCAGCC TCCTCCTCAT 2221 CCCCATCTTC CTCCTCGGCT GTGGTCTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG 2281 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 2341 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAACGAAAGA 2401 TTAGGCACAA GGACTTACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 2461 aagatt